Introduction
Chinese hamster ovary (CHO) cells are one of the most commonly used mammalian cell lines in the biopharmaceutical industry for the production of therapeutic proteins. CHO cells are preferred over other cell lines due to their ability to correctly fold, assemble, and post-translationally modify proteins, which is essential for their biological activity. Genetic engineering of CHO cells has become an increasingly important area of research, as it has the potential to improve the productivity and quality of therapeutic proteins. Genetic engineering of CHO cells modification involves the manipulation of the cell's genome to achieve a specific outcome, such as increased productivity, improved product quality, or altered post-translational modifications. This is achieved by introducing a foreign DNA sequence into the genome of the CHO cell using various techniques, including transfection, electroporation, or viral vectors. The foreign DNA sequence can encode for a protein of interest, a selection marker, or a regulatory element that can modulate gene expression.
CD Biosynsis is a leading provider of synthetic biology services. We will highlight our services for genetic engineering of CHO cells modification, and how we can help our clients achieve their goals in the development of biologics.
Figure 1: Functional genomics for CHO cell engineering. (Fischer S., et al. 2015)
Our Services for Genetic Engineering of CHO Cells Modification
Gene Editing using CRISPR/Cas9 Technology
We provide gene editing services using the latest CRISPR/Cas9 technology. Our team of experts can design and construct CRISPR/Cas9 vectors that target specific genes in the CHO cell genome. Using this technology, we can achieve precise modifications, such as gene knockout or gene insertion, to achieve the desired outcome. Our gene editing services are highly customizable, and we can work with clients to design and optimize the editing strategy to meet their specific needs.
Gene Knockdown using siRNA
We offer gene knockdown services using siRNA, a powerful tool for reducing the expression of a specific gene in the CHO cell genome. We can design and synthesize siRNAs that target specific genes in the CHO cell genome. By reducing the expression of a specific gene, we can achieve specific outcomes, such as increased productivity or altered post-translational modifications.
Gene Overexpression using Viral Vectors or Plasmids
We offer gene overexpression services using viral vectors or plasmids, a common strategy used in genetic engineering to achieve increased productivity of a specific protein. We design and construct viral vectors or plasmids that carry the gene of interest and can be introduced into the CHO cell genome. We can optimize the expression of the gene to achieve the desired outcome, such as increased productivity or altered post-translational modifications.
Cell Line Development
We offer cell line development services to optimize the expression of a specific protein of interest. Our team at CD Biosynsis can develop stable CHO cell lines that express high levels of the target protein and have the desired post-translational modifications. We can optimize the cell culture conditions to achieve the desired outcome, such as increased productivity or altered post-translational modifications.
Cell Engineering for Post-Translational Modifications
We can modify the CHO cell genome to achieve specific post-translational modifications, such as glycosylation, phosphorylation, and acetylation. We can optimize the cell culture conditions to achieve the desired outcome, such as increased productivity or altered post-translational modifications.
Our Advantages
- Expertise in Synthetic Biology
- Customized Solutions
- Comprehensive Services
- High-Quality Results
Genetic engineering of CHO cells modification is an important area of research in the biopharmaceutical industry. At CD Biosynsis, we offer a range of services for the genetic modification of CHO cells, using the latest synthetic biology approaches. Our customized solutions and expertise in biologics development make us a leading provider of synthetic biology services. If you are interested in learning more about our services, please Contact us today.
References
- Xu X., et al. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nature Biotechnology, 2011, 29(8): 735-741.
- Yang Z., et al. Engineered CHO cells for production of diverse, homogeneous glycoproteins. Nature Biotechnology, 2015, 33(8): 842-844.
- Fischer S., et al. The art of CHO cell engineering: A comprehensive retrospect and future perspectives. Biotechnology Advances, 2015, 33(8): 1878-1896.
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