CD Biosynsis is your comprehensive provider for high-quality DNA and RNA Synthesis Services , supporting projects from foundational research to clinical applications. Our state-of-the-art platform ensures sequence accuracy and rapid turnaround times for every product. We offer a full spectrum of services, including: high-fidelity Custom Gene Synthesis and Vector Construction , specialized Modified Oligonucleotide and RNA products (siRNA, sgRNA), and the construction of complex Mutagenesis and CRISPR Libraries . By integrating advanced synthesis techniques with rigorous quality control, we provide the essential nucleic acid tools required for synthetic biology, functional genomics, and therapeutic development.
Get a QuotePrecision in nucleic acid synthesis is paramount. Our high-throughput synthesis pipelines are coupled with proprietary assembly methods to handle complex and long sequences (up to 20kb) with guaranteed 100% sequence accuracy. Furthermore, we offer services like Codon Optimization Service to maximize the expression of your synthesized genes, and specialized purification for RNA and modified oligos to ensure their functional integrity in sensitive applications like in vivo delivery and diagnostics.
Synthesis of any gene from concept to vector, including Custom DNA Synthesis and De Novo Gene Synthesis for novel constructs.
Specialized synthesis for antibody fragments (scFv, Fab) and full-length antibodies, ensuring optimized expression and function.
Seamless assembly of DNA fragments (Gene Fragment Synthesis Service) and insertion into custom vectors using Gateway Cloning and Golden Gate Assembly methods.
Construction of custom plasmids for expression, viral delivery, and genome integration, covering DNA Synthesis and Assembly services.
Synthesis of sequences exceeding 10 kb, including Long DNA Synthesis and ssDNA Synthesis Service , often utilizing Chip-Based DNA Synthesis technology.
Synthesis of standard and specialized primers, including Primer Design and Synthesis , Diagnostic Probes Oligos Synthesis , and NGS Oligos Synthesis .
Oligos with custom modifications (Modified DNA Synthesis Service) including Custom Antisense Oligonucleotides (ASOs) and Custom CpG ODNs Synthesis for therapeutic applications.
Synthesis of functional RNA species, including Custom RNA Oligos Synthesis and Long RNA Synthesis Service .
Specialized RNA interference molecules: Custom miRNA Synthesis , miRNA Agomir/Antagomir Synthesis , and Custom RNA Modifications for enhanced stability.
Comprehensive variant library construction, including Site-Directed Mutagenesis Libraries , Scanning Point Mutation Libraries , Randomized Mutant Library , Degenerated Mutagenesis Libraries , and Precision Mutant Libraries .
Genome-wide and targeted CRISPR libraries: Human Genome Knockout Libraries , Livestock/Poultry CRISPR Libraries , supported by sgRNA Design Service and CRISPR-Cas9 sgRNA Synthesis .
Large-scale assembly projects, including Small Genome Synthesis and Adenovirus Genome Synthesis , along with cDNA Libraries Construction and Synthetic Antibody DNA Libraries .
High-quality, low-bias libraries for functional screening, including oligo pools (Oligo Pool Synthesis Service) and custom DNA libraries.
A systematic workflow to ensure sequence accuracy and reliable product delivery.
Design & Optimization
Synthesis & Assembly
Cloning & QC Verification
Delivery & Documentation
Design Check: Review of sequences for secondary structures and repeats.
Codon Optimization: Optional service to enhance expression in the chosen host organism.
sgRNA Design: Specialized design and validation for high-efficiency CRISPR targets.
Chemical synthesis of oligos, followed by enzymatic and ligation-based assembly for long DNA and genes.
Utilizing proprietary assembly protocols for seamless fusion of fragments without sequence gaps or errors.
Delivery of lyophilized or solution-based DNA (genes, oligos) or RNA (siRNA, sgRNA).
Final report includes sequence maps, alignment files, quality assurance documentation, and purification records.
100% Sequence Guarantee
All genes and assembled constructs are Sanger verified to ensure 100% sequence accuracy , eliminating the need for further QC by the client.
Ultra-High Diversity Libraries
Expertise in building complex Mutagenesis Libraries and sgRNA Libraries with guaranteed coverage and uniformity for high-throughput screening.
Specialized RNA Synthesis
High-purity synthesis and Modification of functional RNA species, including siRNA , miRNA, agomirs, and CRISPR-sgRNA.
Genome-Scale Assembly
Capability to handle projects at the genomic scale, including the De Novo Synthesis and assembly of Small Bacterial and Viral Genomes .
"The Long DNA Synthesis service was crucial for our project. We needed a 15 kb gene assembled perfectly, and CD Biosynsis delivered the vector 100% correct and faster than any other vendor we've tried."
Dr. Emily Tran, PI, Synthetic Genomics Research Lab
"Their expertise in constructing Degenerated Mutagenesis Libraries for our directed evolution screen was exceptional. The diversity was high, and the QC report confirmed minimal sequence bias."
Mr. Alex White, Lead Engineer, Industrial Enzyme Development
"We rely on their high-purity siRNA Synthesis for our in vivo studies. The modified ASOs were delivered with HPLC purity validation and have shown superior stability and efficacy."
Ms. Nina Rodriguez, VP of Preclinical Drug Discovery
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the maximum length of DNA that can be synthesized and assembled?
We routinely deliver highly accurate DNA constructs up to 20 kb using our advanced assembly techniques. For larger projects, our Genome Synthesis and Assembly service handles bacterial or viral genomes up to 200 kb or more.
How do you guarantee the quality of Mutant DNA Libraries ?
We use Next-Generation Sequencing (NGS) on a representative sample of the library to confirm diversity, verify the intended mutation rate, and check for uniform representation of variants, ensuring minimal bias for screening applications.
What kind of RNA modifications can you incorporate?
We offer a wide range of RNA modifications, including 2'-O-Methyl (2'-OMe), Phosphorothioate Bonds (PS), Cholesterol, and various fluorescent labels, crucial for improving the stability, half-life, and delivery efficacy of siRNA and miRNA therapeutics.
Why should I use your Codon Optimization service?
Codon optimization tailors the gene sequence to the transfer RNA pool of the host organism, dramatically increasing the rate and efficiency of protein translation, resulting in up to10-100 times higher expression levels for recombinant proteins.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.