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Trusted by Leading Research & Pharma Institutions

DNA and RNA Synthesis Services

Accelerate your research with custom DNA and RNA oligos consistently manufactured to your exact specifications. From routine PCR primers to complex therapeutic oligonucleotides, we deliver NGS-verified synthetic nucleic acids with industry-leading turnaround times.

NGS Verified
99.9% Accuracy
Fast Turnaround
Learn More

Trusted by leading research and pharmaceutical institutions

Harvard
Pfizer
MIT
Roche
Stanford
Novartis

Why Choose Us

ESI-MS verification on every oligo
1,000+ modification options
90% orders ship within 24 hours
Expert consultation included

Custom Synthesis

Oligos, genes, and modified nucleic acids

Quality Guaranteed

Mass spec and CE verification

Flexible Options

Multiple scales and purifications

Market CAGR
15.8%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive DNA & RNA Synthesis Solutions

Our platform combines established phosphoramidite chemistry with cutting-edge enzymatic synthesis technologies.

Chemical Synthesis

Proven phosphoramidite chemistry with optimized coupling efficiency. Our proprietary synthesis platforms deliver more full-length product than other suppliers, with 99.9% coupling efficiency and rigorous QC at every step.

  • DNA oligos: 5-200 nt
  • RNA oligos: up to 300 nt
  • Gene fragments: up to 15 kb

Enzymatic Synthesis

Next-generation TdT-based synthesis using engineered terminal deoxynucleotidyl transferase with reversible terminators. Template-independent de novo synthesis without toxic reagents.

  • De novo DNA construction
  • Environmentally friendly process
  • Expanded genetic alphabet capability

Complete Coverage

All 20 amino acids at target positions with optimized degenerate codons.

NGS Quality Control

Every library undergoes next-generation sequencing verification with detailed reports.

Flexible Library Types

From single-site saturation to complex combinatorial libraries.

Ready to Accelerate Your Research?

Get a customized quote for your DNA/RNA synthesis project.

Technology Platform

Advanced Synthesis Technologies

Industry-leading synthesis platforms ensuring high-quality output for every project.

Proprietary Platform

Optimized phosphoramidite chemistry with better coupling efficiency than other suppliers, delivering more full-length product.

99.9% Efficiency Automated

Advanced QC Systems

Every oligo undergoes ESI mass spectrometry and capillary electrophoresis to ensure sequence accuracy and purity.

ESI-MS CE Analysis

Enzymatic Synthesis

Next-generation TdT-based synthesis using engineered terminal deoxynucleotidyl transferase with reversible terminators.

De Novo Eco-Friendly

Quality Control

MS Mass spectrometry verification
CE Capillary electrophoresis purity
NGS Next-gen sequencing for genes >1kb
COA Certificate of Analysis included

Purification Options

STD Standard desalting for PCR
CART Cartridge for cloning
HPLC High purity ≥85% for therapeutics
PAGE Ultra-high purity ≥95%
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter DNA Oligonucleotides RNA Oligonucleotides Gene Synthesis
Length Range 5 - 200 nt Up to 300 nt 100 bp - 15 kb
Coupling Efficiency ≥99.5% ≥99.0% NGS verified
Turnaround Time 4-7 business days 7-12 business days 7-17 business days
Scale Range 0.025 - 10 μmol 0.25 - 5 μmol Custom
Purification Desalt, Cartridge, HPLC, PAGE HPLC, PAGE, Dual Standard or custom
Modifications 100+ options 2'-OMe, 2'-F, LNA Codon optimization
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Consultation

Discuss your project requirements and design strategy

2

Design

Sequence optimization and codon design

3

Synthesis

Automated synthesis with real-time monitoring

4

QC

Mass spec, CE, and NGS verification

5

Delivery

Secure packaging with comprehensive report

Applications

Diverse Applications Across Biotechnology

Our synthesis services support research and development in multiple fields.

CRISPR Genome Editing

Custom gRNAs, donor templates, and HDR repair oligos for precise genome engineering applications. Quality matters when you're editing genomes.

  • Single guide RNA (sgRNA) synthesis
  • Donor template design and synthesis
  • HDR repair oligos
  • Chemical modifications for stability
99%
Editing efficiency potential

Therapeutic Oligonucleotides

ASOs, siRNAs, and aptamers with chemical modifications for enhanced stability and cellular delivery. The field has come a long way since the early therapeutic oligonucleotides.

  • Antisense oligonucleotides (ASO)
  • Small interfering RNA (siRNA)
  • Aptamer development
  • Phosphorothioate modifications
100+
Modification options

NGS & Diagnostics

Adapter sequences, capture probes, and qPCR primers for next-generation sequencing and molecular diagnostics. As sequencing costs keep dropping, demand for high-quality oligos keeps growing.

  • NGS adapter and index sequences
  • Capture probes for target enrichment
  • qPCR primers and probes
  • Molecular diagnostic reagents
24h
Fast turnaround
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The NGS-verified synthesis exceeded our expectations. Sequence accuracy was perfect and the QC report was comprehensive. Highly recommended for any genomics project!"

R
Principal Scientist
Biotechnology Company

"Fast turnaround and excellent communication throughout the project. The team provided valuable design recommendations for our CRISPR library. Will definitely use again."

P
Research Director
Academic Research Institution

"We've used this service for multiple therapeutic oligonucleotide projects. Consistent quality and professional service every time. Our go-to provider for oligo synthesis."

L
Lead Researcher
Pharmaceutical Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

316 Citations

Recent progress in non-native nucleic acid modifications

McKenzie LK, El-Khoury R, Thorpe JD, et al. Chemical Society Reviews. 2021.

Comprehensive review of modified nucleic acids for therapeutic oligonucleotides including ASOs and siRNAs.

View DOI
106 Citations

Enzymatic DNA synthesis by engineering terminal deoxynucleotidyl transferase

Lu X, Li J, Li C, Lou Q, Peng K, Cai B, Liu Y, Yao Y, et al. ACS Catalysis. 2022.

Engineering TdT for enzymatic DNA synthesis with reversible terminators enabling high-throughput automated production.

View DOI
96 Citations

Catalytic asymmetric and stereodivergent oligonucleotide synthesis

Featherston AL, Kwon Y, Pompeo MM, Engl OD, et al. Science. 2021.

Breakthrough in catalytic asymmetric synthesis of all possible oligonucleotide stereoisomers.

View DOI
57 Citations

Automated high-throughput DNA synthesis and assembly

Ma Y, Zhang Z, Jia B, Yuan Y. Heliyon (Cell Press). 2024.

Comprehensive review of automated synthesis platforms and biofoundries for accurate high-throughput DNA production.

View DOI
109 Citations

Chemical synthesis and biological application of modified oligonucleotides

Glazier DA, Liao J, Roberts BL, Li X, Yang K, et al. Bioconjugate Chemistry (ACS). 2020.

Review of chemical synthesis methods for RNA and modified oligonucleotides with simplified workflows.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

Chemical synthesis uses phosphoramidite chemistry on solid supports, offering high efficiency and mature protocols. Enzymatic synthesis uses terminal deoxynucleotidyl transferase (TdT) with reversible terminators, enabling template-independent de novo synthesis without toxic reagents. Recent advances in TdT engineering have improved enzymatic synthesis efficiency for commercial applications.
All syntheses undergo rigorous quality control including mass spectrometry (MALDI-TOF or ESI-MS) and capillary electrophoresis for purity analysis. Gene constructs longer than 1 kb receive NGS verification. Our automated platforms achieve ≥99.5% coupling efficiency, and every order includes a detailed QC report.
We offer over 100 chemical modifications including: backbone modifications (phosphorothioate), sugar modifications (2'-O-methyl, 2'-F, LNA), fluorescent dyes (FAM, HEX, Cy3, Cy5), quenchers (BHQ1, BHQ2), and various spacer groups. Modified nucleic acids have shown enhanced stability and therapeutic potential.
Standard DNA oligonucleotides: 4-7 business days. RNA oligonucleotides: 7-12 business days. Gene synthesis: 7-17 business days depending on complexity and length. Rush services are available for urgent projects. >90% of small-scale DNA orders ship within 48 hours.
Yes, we provide free codon optimization for any host organism. Our algorithms consider codon usage bias, GC content, mRNA secondary structure, and repetitive sequences to maximize protein expression. Custom optimization parameters can be specified for special requirements.
For standard PCR primers, desalting is sufficient. For cloning and sequencing, cartridge purification is recommended. For therapeutic or diagnostic applications requiring high purity, we recommend HPLC (≥85% purity) or PAGE (≥95% purity). Our team can advise on the best option for your specific application.

Ready to Start Your Project?

Get a customized quote for your DNA and RNA Synthesis Services project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Custom DNA Synthesis
· Custom RNA Synthesis
· DNA Variant Library Construction
· Genome Synthesis and Assembly

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
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