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Trusted by Leading Research & Pharma Institutions

RNA Interference (RNAi) Services

Accelerate your research with custom siRNA consistently manufactured to your exact specifications. From functional genomics screening to therapeutic development, we deliver high-quality synthetic small interfering RNAs with industry-leading knockdown efficiency and minimal off-target effects.

≥75% Knockdown
Minimal Off-Target
100+ Modifications
Learn More

Trusted by leading research and pharmaceutical institutions

Stanford
Novartis
MIT
Roche
Johns Hopkins
Pfizer

Why Choose Us

Guaranteed ≥75% knockdown at mRNA level
100+ chemical modification options
SMARTpool technology for enhanced potency
Expert consultation included

Custom siRNA

21-23 bp duplex, any sequence

Quality Guaranteed

Mass spec and HPLC verification

Flexible Options

Multiple scales and modifications

Market CAGR
12.5%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive RNA Interference Solutions

Our platform combines advanced siRNA design algorithms with cutting-edge chemical synthesis technologies to deliver highly specific gene silencing reagents.

Custom siRNA Synthesis

Tailored synthesis of siRNA sequences targeting specific genes of interest. Our proprietary design algorithms ensure optimal knockdown efficiency with minimal off-target effects across human, mouse, rat, and custom species.

  • 21-23 bp siRNA duplex
  • 100+ modification options
  • SMARTpool technology available

Quality Guaranteed

Every siRNA undergoes rigorous quality control including mass spectrometry verification and HPLC purity analysis. Guaranteed ≥75% knockdown at mRNA level ensures reliable experimental results.

  • ESI-MS verification on every duplex
  • HPLC purity ≥85%
  • COA included with delivery

Smart Design

Advanced algorithms minimize off-target effects through seed region analysis and thermodynamic optimization.

Complete QC

Mass spectrometry, HPLC, and CE analysis ensure sequence accuracy and purity.

Flexible Options

From research-scale to GMP manufacturing, multiple scales and purification levels available.

Ready to Accelerate Your Research?

Get a customized quote for your RNAi project.

Technology Platform

Advanced RNAi Technologies

Industry-leading design algorithms and synthesis platforms ensuring high-quality output for every project.

Smart Design Algorithm

Proprietary algorithms incorporating seed region filters and thermodynamic analysis for optimal siRNA selection with minimal off-target effects.

Seed Analysis Thermodynamic

Quality Verification

Every siRNA undergoes ESI mass spectrometry and HPLC analysis to ensure sequence accuracy and purity levels meet specifications.

ESI-MS HPLC

Chemical Modifications

Comprehensive modification options including 2'-O-Me, 2'-F, phosphorothioate, LNA, and delivery conjugates for enhanced stability and efficacy.

2'-OMe PS LNA

Quality Control

MS Mass spectrometry verification
HPLC High purity ≥85%
CE Capillary electrophoresis
COA Certificate of Analysis included

Purification Options

DSL Desalted for standard applications
HPLC High purity ≥85%
PAGE Ultra-high purity ≥95%
Duplex Annealed and purified duplex
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter Standard siRNA Modified siRNA SMARTpool
Length 21-23 bp duplex 21-23 bp duplex 4 siRNAs per pool
Purity ≥85% (HPLC) ≥85% (HPLC) ≥85% (each)
Scale Range 0.05-10 μmol 0.1-5 μmol 0.05-2 μmol
Modifications Standard 100+ options Customizable
Purification DSL, HPLC, PAGE HPLC, PAGE DSL, HPLC
Knockdown Guarantee ≥75% (mRNA) ≥75% (mRNA) ≥75% (pool)
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Consultation

Discuss project requirements and design strategy

2

Design

Algorithm optimization and modification selection

3

Synthesis

Automated synthesis with real-time monitoring

4

QC

Mass spec, HPLC, and purity verification

5

Delivery

Lyophilized product with comprehensive report

Applications

Diverse Applications Across Biotechnology

Our synthesis services support research and development in multiple fields.

Functional Genomics Screening

High-throughput siRNA screening for systematic gene function analysis. Our SMARTpool technology and validated design algorithms ensure reliable, reproducible results across genome-wide or targeted screens.

  • Genome-wide screening libraries
  • Targeted pathway analysis
  • Drug target identification
  • LncRNA functional studies
≥75%
Guaranteed knockdown

RNAi Therapeutic Development

From early research to clinical development, our comprehensive siRNA synthesis supports the growing field of RNA therapeutics. Advanced chemical modifications enhance stability, delivery, and therapeutic efficacy.

  • GalNAc conjugate synthesis
  • Phosphorothioate modifications
  • 2'-O-Me and 2'-F stabilization
  • GMP manufacturing available
100+
Modification options

Drug Discovery & Validation

Rapid siRNA synthesis for target validation and lead optimization in drug discovery pipelines. Our guaranteed knockdown and minimal off-target effects ensure reliable data for informed decision-making.

  • Target validation studies
  • Mechanism of action studies
  • Resistance mechanism identification
  • Biomarker discovery
5-7
Business days turnaround
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The SMARTpool technology significantly improved our screening results. Consistent knockdown efficiency and minimal off-target effects made our genome-wide screen a success."

J
Senior Scientist
Biotechnology Company

"Excellent service and product quality. The modification options available helped optimize our siRNA for in vivo delivery applications. Customer support was very responsive."

M
Research Director
Pharmaceutical Company

"We've been using their siRNA services for years for our cancer research. The knockdown guarantee gives us confidence in our experimental results."

L
Principal Investigator
Academic Research Institution
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

156 Citations

Programmably tiling rigidified DNA brick on gold nanoparticle as multi-functional shell for cancer-targeted delivery of siRNAs

Xue C, Hu S, Gao ZH, et al. Nature Communications. 2021.

Development of Y-shaped DNA bricks with gold nanoparticles for targeted siRNA delivery, enabling enhanced gene silencing in cancer therapy applications.

View DOI
89 Citations

Conformation-sensitive targeting of lipid nanoparticles for RNA therapeutics

Dammes N, Goldsmith M, Ramishetti S, et al. Nature Nanotechnology. 2021.

Development of conformation-sensitive targeting strategies for lipid nanoparticles enabling selective RNA therapeutics delivery to specific cell types.

View DOI
67 Citations

DNA-inspired nanomaterials for enhanced endosomal escape

Lee J, Sands I, Zhang W, Zhou L, Chen Y. Proceedings of the National Academy of Sciences. 2021.

Development of Janus base nanotubes enabling efficient siRNA delivery with enhanced endosomal escape and reduced cytotoxicity.

View DOI
45 Citations

siRNA delivery using intelligent chitosan-capped mesoporous silica nanoparticles for overcoming multidrug resistance

Heidari R, Khosravian P, Mirzaei SA, Elahian F. Scientific Reports. 2021.

Development of chitosan-capped mesoporous silica nanoparticles for effective siRNA delivery to overcome cancer multidrug resistance.

View DOI
38 Citations

Engineering siRNA therapeutics: challenges and strategies

Zaidi SSA, Fatima F, Zaidi SAA, Zhou D, Deng W, Liu S. Journal of Nanobiotechnology. 2023.

Comprehensive review of siRNA engineering strategies including chemical modifications, delivery systems, and therapeutic applications.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our RNAi services.

What chemical modifications improve siRNA stability?
Common modifications include 2'-O-methyl (2'-OMe), 2'-fluoro (2'-F), phosphorothioate linkages, and locked nucleic acids (LNA). These modifications enhance nuclease resistance, improve serum stability, and can reduce off-target effects. We offer 100+ modification options to optimize your siRNA for specific applications.
How does SMARTpool technology improve knockdown efficiency?
SMARTpool combines four distinct siRNAs targeting the same gene into a single reagent. This approach improves the likelihood of effective gene silencing, minimizes sequence-specific off-target effects by lowering the relative concentration of each individual siRNA, and better mimics the natural RNAi pathway. Research shows SMARTpool formats achieve more consistent and robust knockdown results.
What delivery options are available for difficult-to-transfect cells?
For challenging cell types such as neurons, primary cells, and suspension cells, we offer self-delivering siRNA modifications that enable uptake without transfection reagents. Additionally, we provide various conjugation options including GalNAc for liver targeting, cell-penetrating peptides, and lipid modifications to enhance cellular delivery.
What quality control do you perform on siRNA products?
Every siRNA undergoes comprehensive quality control including ESI mass spectrometry for sequence verification, HPLC analysis for purity assessment (≥85%), and capillary electrophoresis for strand integrity. Certificate of Analysis with detailed QC data is included with each delivery.
Do you offer GMP manufacturing for therapeutic development?
Yes, we offer GMP manufacturing services for clinical and commercial applications. Our facilities are ISO 9001 and ISO 13485 certified, with FDA-inspected cGMP manufacturing capabilities. We support projects from early research phase through late-stage clinical development with comprehensive regulatory documentation.

Ready to Start Your Project?

Get a customized quote for your RNA Interference (RNAi) Services project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

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· Genome Editing Service
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· TALEN Gene Editing Services

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
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