Protein expression in E. coli service
Protein expression in E. coli is a widely used technique in molecular biology and biotechnology. It involves the production of a specific protein of interest using the bacterium Escherichia coli (E. coli) as the host organism. E. coli offers several advantages for protein expression, including ease of genetic manipulation, rapid growth, and cost-effectiveness.
Protein folding and secretion in E. coli(HP Sørensen, et al.,2005)
Protein expression in E. coli plays a crucial role in various research fields and industries. It enables scientists and researchers to produce large quantities of proteins for a wide range of applications. By harnessing the power of E. coli, researchers can study the structure and function of proteins, develop new therapies and drugs, and contribute to advancements in fields such as pharmaceutical research, enzymology, and structural biology.
Services Offered
Service Process
FAQs
Services Offered
There are different types of protein expression systems in E. coli, each with its own advantages and applications. Inducible expression systems, for example, allow researchers to control the timing and level of protein expression, providing more flexibility in experimental design. Fusion protein expression systems, on the other hand, enable the production of proteins fused with specific tags or domains, facilitating protein purification and characterization.
| Applications |
Description |
| - General protein production |
- Description: Constitutive expression systems in E. coli are commonly used for the routine production of proteins. This includes the generation of proteins for research purposes, structural studies, or industrial applications. |
| - High-throughput screening |
- Description: Constitutive expression and inducible systems in E. coli are employed for high-throughput screening of proteins. This allows researchers to quickly generate and analyze large numbers of protein variants or conduct functional studies on a broad scale. |
| - Controlled protein expression |
- Description: Inducible expression systems in E. coli are valuable when precise control of protein expression is required. Researchers can optimize growth conditions and induce protein expression at specific time points, allowing for controlled and regulated production. |
| - Optimizing growth conditions |
- Description: Inducible systems are particularly useful for optimizing growth conditions during protein expression. Researchers can fine-tune induction parameters, such as inducer concentration and induction timing, to achieve optimal protein yields and characteristics. |
| - Protein solubility improvement |
- Description: Fusion protein expression systems in E. coli, involving the addition of solubility-enhancing tags or partners, are applied to improve the solubility of target proteins. This is especially important for proteins prone to forming inclusion bodies or aggregates. |
| - Fusion protein expression systems |
- Description: Fusion protein expression systems also facilitate affinity purification. By incorporating tags like GST or His-tag, researchers can easily purify the expressed proteins using affinity chromatography, streamlining the purification process and enhancing protein yield. |
| - Simplified protein expression |
- Description: Autoinduction systems simplify protein expression in E. coli by using media containing lactose. This allows for time-controlled induction without constant monitoring, making the process of expressing proteins more straightforward and convenient. |
| - Time-controlled induction |
- Description: Autoinduction systems allow researchers to control protein expression over time without the need for constant monitoring. This is beneficial for high-throughput applications or situations where precise control of protein expression timing is critical. |
| - Improved solubility |
- Description: The Sumo Fusion System in E. coli is utilized to improve the solubility of target proteins. The Small Ubiquitin-like Modifier (SUMO) tag aids in proper folding, preventing aggregation, and enhancing solubility, particularly for proteins that are challenging to express. |
If you have any more questions or would like to discuss your specific protein expression requirements, please don't hesitate to contact us. We are here to assist you in achieving your research goals.
Service Process
Our company offers Protein Expression in E. coli as a comprehensive service to meet your protein production needs. We have a streamlined process in place to ensure efficient and high-quality protein expression. Here are the steps involved in our service process:
- Project consultation: We work closely with our clients to understand their protein expression needs and objectives. During this phase, we discuss the desired protein target, expression system requirements, and any specific modifications or tags needed.
- Gene optimization and synthesis: If required, we optimize and synthesize the target gene to enhance protein expression in E. coli. This step ensures efficient translation and proper folding of the protein of interest.
- Plasmid construction: We clone the optimized gene into an expression vector and prepare the necessary plasmids. The choice of expression vector depends on factors such as protein size, desired expression level, and downstream applications.
- Transformation and expression: The recombinant plasmids are transformed into E. coli cells, which serve as the host organism for protein expression. We carefully select the appropriate E. coli strain and optimize the culture conditions to maximize protein production.
- Protein purification: After successful expression, we employ various purification techniques to isolate and purify the protein of interest. This step ensures the removal of contaminants and yields a highly pure protein sample.
- Quality control: The purified protein undergoes rigorous quality control to ensure its integrity and functionality. We perform assays to assess protein purity, stability, and activity, ensuring that the final product meets the highest quality standards.
- Delivery: The final purified protein is delivered to the client along with comprehensive documentation, including protocols, quality control data, and any additional information required for downstream experiments or applications.
For more information or to discuss your specific protein expression requirements, please feel free to contact us. We are dedicated to providing high-quality protein expression services tailored to your research needs.
FAQs
Here are some frequently asked questions about our Protein Expression in E. coli service:
Q: Can you express proteins with post-translational modifications in E. coli?
A: E. coli is limited in its ability to perform post-translational modifications; however, our team can assist in alternative strategies such as co-expression with modifying enzymes or utilizing E. coli strains engineered for specific modifications. We can discuss the best approach based on your specific requirements.
Q: How long does the protein expression process take?
A: The timeline for protein expression in E. coli can vary depending on various factors, including the complexity of the protein and the expression system used. Our experienced team will provide an estimated timeline specific to your project during the initial consultation phase. We strive to deliver high-quality results in a timely manner.
Q: Can you handle large-scale protein expression projects?
A: Yes, our facility is equipped to handle both small-scale and large-scale protein expression projects. We have state-of-the-art equipment and expertise to meet diverse project requirements. Whether you need a few milligrams or grams of protein, we can scale up the production process accordingly.
Q: Do you provide protein characterization services as well?
A: Yes, we offer comprehensive protein characterization services to complement our protein expression service. Our range of characterization techniques includes protein structure analysis, activity assays, and protein-protein interaction studies. These services provide valuable insights into the properties and functions of your protein of interest, enhancing the understanding of its biological role and potential applications.
Q: What are the typical deliverables for the Protein Expression in E. coli service?
A: The typical deliverables for our Protein Expression in E. coli service include the purified protein sample, comprehensive documentation (protocols, quality control data), and any additional information required for downstream experiments or applications. We ensure that you receive all the necessary materials and information to further your research.
Q: Can you assist with protein expression troubleshooting and optimization?
A: Yes, our experienced team can assist with troubleshooting and optimization of protein expression in E. coli. If you encounter difficulties or suboptimal expression levels, we can work closely with you to identify and address any issues. Our goal is to help you achieve successful protein expression and obtain high-quality results.
Q: Are there any protein size limitations for expression in E. coli?
A: E. coli expression systems are generally well-suited for proteins of moderate size. However, very large or complex proteins may pose challenges in terms of expression efficiency and solubility. During the project consultation phase, we assess the feasibility of expressing your protein of interest in E. coli and discuss alternative strategies if necessary.
Q: Can you assist with downstream applications of the expressed proteins?
A: Absolutely! In addition to our Protein Expression in E. coli service, we offer a wide range of downstream protein services to support your research. This includes protein characterization, structural analysis, activity assays, and protein-protein interaction studies. Our comprehensive suite of services ensures that you have the necessary tools and insights to explore the properties and functions of your expressed proteins.
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