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Plant Molecular Farming

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Introduction of Plant Molecular Farming

Plant molecular farming is a rapidly growing field in synthetic biology that utilizes plants as bioreactors for the production of recombinant proteins. This innovative approach presents many advantages over traditional mammalian-based expression systems, such as lower costs, reduced risk of contamination, and increased scalability. At CD Biosynsis, we specialize in providing high-quality services for plant molecular farming, leveraging our expertise in synthetic biology and plant biotechnology to offer customized solutions for our clients.

Overview of plant transformation approaches employed for the production of recombinant pharmaceutical and non-pharmaceutical proteins in plants. Figure 1: Overview of plant transformation approaches employed for the production of recombinant pharmaceutical and non-pharmaceutical proteins in plants. (Shanmugaraj B., et al. 2020)

Our Services for Plant Molecular Farming

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Gene Synthesis and Cloning

We offer gene synthesis and cloning services for the expression of recombinant proteins in plant-based systems. Our team of experts can synthesize genes for optimal expression and cloning into plant expression vectors. We utilize advanced gene synthesis technologies, such as oligonucleotide synthesis and PCR amplification, to ensure high-quality gene synthesis and cloning.

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Plant Transformation

We specialize in plant transformation, utilizing a variety of methods to introduce recombinant genes into plant cells. Our team of experts can optimize transformation protocols, including Agrobacterium-mediated transformation and biolistic transformation, to ensure maximum efficiency and yield. We have extensive experience in the transformation of a wide range of plant species, including Arabidopsis, tobacco, maize, and rice.

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Protein Expression and Purification

At CD Biosynsis, we specialize in optimizing plant-based production systems for maximum recombinant protein expression and yield.

We offer a variety of protein expression systems, including plastid-based and nuclear-based expression, to ensure optimal expression of recombinant proteins.

We also offer protein purification services, utilizing a variety of methods, such as ion exchange chromatography, size exclusion chromatography, and affinity chromatography, to ensure high purity and yield.

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Quality Control

We utilize stringent quality control measures to ensure that our plant-based production systems remain free from contaminants and provide high-quality, pure recombinant proteins. We offer a variety of quality control services, including protein characterization, purity analysis, and contamination testing. We use equipment, such as HPLC, SDS-PAGE, and ELISA, to ensure that our recombinant proteins meet the highest quality standards.

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Customized Solutions

Our team of experts can work closely with our clients to develop customized protocols for gene synthesis, cloning, transformation, protein expression, and purification, ensuring that we meet the unique needs of each client.

Our Advantages

  • Cost-Effective Production:
    • Utilization of low-cost, scalable plant-based production systems.
    • Reduction in production costs compared to traditional mammalian-based systems.
    • Maximization of efficiency and cost-effectiveness through optimization of plant-based production systems.
  • Reduced Risk of Contamination:
    • Utilization of plants that do not harbor the same pathogens as mammalian cells.
    • Stringent quality control measures to ensure high-quality, pure recombinant proteins.
    • Lower risk of contamination compared to traditional mammalian-based expression systems.
  • Increased Scalability:
    • Utilization of large-scale, low-cost environments for plant growth, such as greenhouses and open fields.
    • Optimization of plant-based production systems for scalability.
    • Ability to achieve high levels of recombinant protein expression on a large scale.
  • Customized Solutions:
    • Tailored protocols and solutions for each client's specific needs and requirements.

CD Biosynsis offers a comprehensive range of services for plant molecular farming, leveraging our expertise in synthetic biology and plant biotechnology to provide customized solutions for our clients. Our services include gene synthesis and cloning, plant transformation, protein expression and purification, quality control, and customized solutions. If you are interested in learning more about our services, please Contact us today.

References

  1. Obembe Olawole O., et al. Advances in plant molecular farming. Biotechnology Advances, 2011, 29(2): 210-222.
  2. Shanmugaraj B., et al. Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production. Plants (Basel, Switzerland), 2020, 9(7), 842.

Please note that all services are for research use only. Not intended for any clinical use.

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