Base Editing Service

Base Editing is a revolutionary genome editing technology that enables precise, single-base conversions without introducing a DNA Double-Strand Break. This innovation allows scientists to directly correct pathogenic single-nucleotide polymorphisms, which are responsible for a significant portion of human genetic diseases. By minimizing the formation of insertion/deletion by products associated with traditional CRISPR/Cas9 systems, Base Editing offers unmatched fidelity and safety, making it a critical tool for advancing both fundamental research and clinical-stage therapeutic development.

CD Biosynsis offers end-to-end, customized Base Editing CRO services, leveraging state-of-the-art Adenine Base Editors and Cytosine Base Editors platforms. Our goal is to translate your complex research requirements into highly predictable and validated genomic outcomes. From sophisticated gRNA design and construct optimization to rigorous quality control and model delivery, our expert team ensures that your project benefits from unparalleled technical precision and seamless project management, significantly accelerating your drug discovery pipeline.

Highlights

We are a trusted CRO partner specializing in gene editing, committed to delivering clinical-grade precision and efficiency for your therapeutic programs.

• Minimizing Off-Target Risk: We specialize in DSB-free editing, drastically reducing the formation of INDELs and chromosomal aberrations which are critical concerns in therapeutic development.
• CRO Expertise: Benefit from our streamlined project workflow, dedicated project managers, and robust documentation support for preclinical and regulatory submissions.
• Validated Excellence: We guarantee high on-target editing efficiency (often exceeding 80%), supported by comprehensive NGS data and quality reports.
• Customization Focus: Every project receives a tailored strategy, selecting the optimal editor (ABE or CBE) and delivery system (RNP, LNP, or viral vector) based on your unique cell line and objective.

Applications

Our Base Editing CRO services accelerate discovery across various therapeutic and research fields:

Genetic Disease Modeling and Correction

           

Correcting specific pathogenic single-nucleotide polymorphisms in patient-derived cells, particularly for monogenic disorders.

Drug Target Validation

Introducing specific, subtle point mutations in cell lines to accurately mimic natural disease states or confirm the therapeutic relevance of a target.

Cell Therapy Engineering (e.g., CAR-T/NK)

Precise gene inactivation (knockout) by introducing premature stop codons to enhance safety, specificity, or persistence of engineered immune cells.

Functional Genomics Studies

Creating libraries of precise point mutations to systematically analyze protein function, regulatory elements, and pathway mechanisms.

Solutions

CD Biosynsis provides a complete suite of services designed to achieve precise genomic outcomes.

Optimized Base Editor System Design

Expert design and rational engineering of ABE and CBE variants for maximized efficiency and reduced bystander editing.

High-Fidelity gRNA Design and Screening

Advanced bioinformatics and experimental screening to identify gRNA sequences with the highest specificity and efficiency for your target locus.

Cell Line Engineering and Correction

Generation of stable cell lines and precise iPSC correction for single-base mutations, suitable for disease modeling and drug screening.

Delivery System Optimization

Tailoring the most effective delivery format, including optimized RNP complexes, proprietary LNP formulations, and high-titer viral vectors.

Off-Target Effect Prediction and Validation

Rigorous computational analysis and wet-lab deep sequencing to monitor and minimize unintended edits across the genome.

Workflow

CD Biosynsis's Base Editing CRO service follows a rigorous, multi-step workflow designed for precision and predictability:

• Initial Consultation and Strategy Design: We begin with a deep discussion of your research objectives, target gene, host cell line, and the specific single-base correction required. Our team then designs the optimal Base Editor (ABE or CBE) strategy and gRNA sequences to ensure maximum on-target specificity.
• Construct Generation and Delivery Optimization: We synthesize and validate all necessary Base Editor constructs and gRNA. Concurrently, we optimize the delivery method—whether it is an RNP complex, an LNP formulation, or a viral vector—to achieve the highest possible efficiency for your specific cell line.
• Cell Line Editing and Clone Isolation: The optimized editing constructs are delivered into the host cells. We employ high-throughput screening and clonal isolation techniques to successfully generate and select single-cell derived, edited clones.
• Rigorous Quality Control and Validation: All presumptive positive clones undergo comprehensive verification. We use advanced deep sequencing (NGS and Sanger sequencing) to confirm the desired on-target editing efficiency, verify the edit's homozygosity, and rigorously screen for potential off-target mutations.
• Final Delivery and Documentation: Upon passing all stringent QC metrics, the project is finalized. We deliver the validated edited cell line/model to you, along with a complete data package including the comprehensive QC report, genetic maps, full sequencing data, and detailed protocols.

CD Biosynsis maintains strict quality control measures compliant with industry standards to ensure the reliability and translatability of your results. Every project includes:

• Guaranteed Efficiency: Documentation of high on-target editing rates specific to your cell line and target.
• Comprehensive Data Package: Full raw and analyzed data from NGS to prove single-base conversion and assess the risk of INDELs and off-target edits.
• Reproducible Protocols: Detailed, written standard operating procedures (SOPs) and material specifications to ensure reproducibility in your own lab or in subsequent clinical development stages.
• Project Transparency: Dedicated Project Managers provide scheduled updates and are available for immediate consultation throughout the project life cycle.