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Trusted by Leading Research & Pharma Institutions

Custom RNA Modification Services

Accelerate your RNA research with precisely engineered modifications — from pseudouridine to m6A, 2'-F, LNA, and beyond. Our specialized phosphoramidite platforms deliver chemically modified RNA oligos with site-specific control, mandatory HPLC purification, and mass spectrometry verification for every order.

Site-Specific
200+ Modifications
HPLC + MS Verified
Learn More

Trusted by leading research and pharmaceutical institutions

MIT
Pfizer
Stanford
Roche
Johns Hopkins
Novartis

Why Choose Us

Site-specific modification placement
200+ modification chemistries available
HPLC purification for every order
ESI-MS verification on every oligo

Precision Chemistry

Site-specific modifications at 5', 3', or internal positions

Quality Guaranteed

Mass spec and HPLC dual verification

Flexible Options

Multiple scales, purities, and formats

Market CAGR
22.4%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Precision RNA Modifications for Advanced Research

Our specialized RNA modification service combines cutting-edge phosphoramidite chemistry with site-specific control to deliver the modified RNA your research demands.

Chemical Synthesis Platform

Our proprietary phosphoramidite synthesis platforms deliver more full-length modified product than standard suppliers. Specialized chemistry handles complex modifications including base analogs, sugar modifications, and backbone alterations with precision coupling efficiency.

  • ssRNA oligos: 5-200 nt
  • siRNA and microRNA constructs
  • CRISPR guide RNAs with chemical modifications

Site-Specific Precision

Every modified RNA is synthesized with modification placed at exactly your specified position — 5', 3', or internally — ensuring reproducible functional results. RNase-free conditions are maintained throughout the entire manufacturing process.

  • Single or multiple modifications per oligo
  • Compatible with challenging secondary structures
  • Expert consultation for optimal design

Complete Modification Coverage

Over 200 modification chemistries including m6A, pseudouridine, 2'-OMe, 2'-F, LNA, PS, and fluorescent probes.

Dual QC Verification

Every modified RNA undergoes ESI-MS verification plus HPLC purity analysis with detailed documentation.

Flexible Scales & Formats

From nanomole research scale to multi-gram therapeutic development with tubes or barcoded plates.

Ready to Engineer Your RNA Modifications?

Get a customized quote for your modified RNA project today.

Technology Platform

Advanced Modification Synthesis Technologies

Industry-leading platforms delivering precision RNA modifications with unmatched coupling efficiency.

Proprietary Phosphoramidite Platform

Optimized phosphoramidite chemistry with specialized protocols for sensitive modifications, delivering more full-length product with higher coupling efficiency than standard methods.

≥99.0% Efficiency Automated

Dual QC Verification

Every modified RNA undergoes both electrospray ionization mass spectrometry (ESI-MS) and HPLC analysis to confirm exact mass shifts and purity levels.

ESI-MS HPLC Purity

Modified IVT Capability

For long RNA and mRNA constructs, our modified in vitro transcription service incorporates pseudouridine, m1Ψ, and other nucleoside modifications during synthesis.

Pseudouridine m1Ψ

Modification Categories

Sugar 2'-OMe, 2'-F, 2'-MOE, LNA, cEt, BNA
Base m6A, m5C, pseudouridine, m1Ψ, inosine, s²U
Backbone Phosphorothioate (PS), PO/PS mix, PN
Labels FAM, Cy3, Cy5, BHQ, biotin, quenchers

Purification Options

HPLC Standard for modified RNA
PAGE For complex modified sequences
Dual HPLC + PAGE for ultra-complex
COA Certificate of Analysis included
Specifications

Flexible Options for Diverse Research Needs

Comprehensive specifications to meet your modification requirements.

Parameter Standard Modified RNA siRNA / Duplex CRISPR Guide RNAs
Length Range 5 - 200 nt 19 - 30 bp duplex Up to 100 nt
Modification Types 200+ options 2'-F, PS, 2'-OMe m6A, ψ, PS, labels
Coupling Efficiency ≥99.0% per step High-fidelity Site-specific
Scale Range 10 nmol - 10 μmol 50 nmol - 1 μmol Custom scale
Purification HPLC (standard) HPLC, dual purification HPLC, PAGE
QC Verification ESI-MS + HPLC MS + PAGE + anneal QC MS + HPLC + CE
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Consultation

Discuss modification strategy and design optimization

2

Design

Site-specific placement and phosphoramidite selection

3

Synthesis

Automated phosphoramidite synthesis in RNase-free conditions

4

QC

ESI-MS mass verification and HPLC purity analysis

5

Delivery

Lyophilized with COA, MS report, and HPLC data

Applications

Diverse Applications Across Research & Therapeutics

Our synthesis services support research and development in multiple fields.

RNA Therapeutics Development

ASOs, siRNAs, and aptamers with precisely placed chemical modifications for enhanced pharmacokinetics, cellular stability, and therapeutic efficacy. Modified RNA is the foundation of next-generation nucleic acid drugs.

  • Antisense oligonucleotides (ASO) with PS backbone
  • siRNA with 2'-F and 2'-OMe modifications
  • Aptamers with LNA and nuclease-resistant links
  • mRNA with pseudouridine and m1Ψ for reduced immunogenicity
200+
Modification options

CRISPR Genome Editing

Chemically modified guide RNAs (gRNAs) for improved stability, specificity, and editing efficiency. Modified gRNAs have demonstrated enhanced performance in both research and therapeutic applications.

  • sgRNA and crRNA with 2'-F or 2'-OMe modifications
  • Base editing and ADAR-recruiting RNAs
  • HDR repair templates with PS linkages
  • pegRNA with site-specific chemical labels
99%
Editing efficiency potential

Molecular Diagnostics

High-performance diagnostic probes and molecular beacons combining fluorophores and quenchers for qPCR, FISH, and next-generation sequencing applications. Precision modification ensures optimal assay performance.

  • TaqMan-style dual-labeled probes
  • Molecular beacons with hairpin quencher design
  • NGS adapters and capture probes
  • Epitranscriptomics study probes (m6A, ψ)
High
Fluorophore-quencher pairing
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The site-specific modification was exactly what our epitranscriptomics project needed. The MS verification gave us complete confidence that the m6A was incorporated at the right position. Excellent service."

S
Senior Research Scientist
Biotechnology Company

"We needed heavily modified siRNA with multiple PS linkages and fluorophore labels. The team provided expert consultation and delivered exactly what we needed. Highly recommended for complex RNA projects."

M
Principal Investigator
Academic Research Institution

"We've relied on this service for our CRISPR guide RNA modifications across multiple therapeutic programs. The consistent quality and detailed QC documentation have been invaluable for our IND-enabling studies."

L
Research Director
Pharmaceutical Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

316 Citations

Recent progress in non-native nucleic acid modifications

McKenzie LK, El-Khoury R, Thorpe JD, et al. Chemical Society Reviews. 2021.

Comprehensive review covering non-native modifications in ASOs, siRNA, aptamers, and XNA synthesis technologies.

View DOI
187 Citations

Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines

Nance KD, Meier JL. ACS Central Science. 2021.

Review of m1Ψ modification in COVID-19 mRNA vaccines, explaining immune evasion and enhanced protein translation mechanisms.

View DOI
142 Citations

Systematic calibration of epitranscriptomic maps using a synthetic modification-free RNA library

Zhang Z, Chen T, Chen HX, et al. Nature Methods. 2021.

Development of synthetic modification-free RNA controls for precise calibration of m6A and m5C epitranscriptomic mapping methods.

View DOI
109 Citations

Chemical Synthesis and Biological Application of Modified Oligonucleotides

Glazier DA, Liao J, Roberts BL, et al. Bioconjugate Chemistry (ACS). 2020.

Review of RNA chemical synthesis methods and how modifications address stability and delivery challenges for biological applications.

View DOI
89 Citations

Synthesis of Nucleobase-Modified RNA Oligonucleotides by Post-Synthetic Approach

Bartosik K, Debiec K, Czarnecka A, et al. Molecules. 2020.

Review highlighting post-synthetic RNA modification approaches for introducing nucleobase modifications including m6A, fluorescent dyes, and spin labels.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

We offer 200+ modification options across four main categories: sugar modifications (2'-OMe, 2'-F, 2'-MOE, LNA, cEt), base modifications (m6A, m5C, pseudouridine, m1Ψ, inosine, s²U), backbone modifications (phosphorothioate), and fluorescent labels (FAM, Cy3, Cy5, BHQ, biotin). Modifications can be placed at 5', 3', or internal positions.
Every modified RNA undergoes ESI-MS mass spectrometry verification. Since each chemical modification results in a specific mass shift, MS is the definitive method to confirm that the correct number and type of modifications were incorporated at the specified positions. HPLC purity analysis further confirms the full-length product.
Yes, we routinely synthesize complex RNA that combines different chemistries — for example, phosphorothioate backbone modifications for stability, 2'-OMe at the 5' end for exonuclease resistance, and a fluorophore at the 3' end, all in a single molecule. Our expert consultation helps you optimize the design for synthesis success.
LNA (Locked Nucleic Acid) locks the ribose ring in a 3'-endo conformation. This significantly increases the thermal stability (Tm) of the RNA when hybridized to complementary DNA or RNA, leading to superior binding affinity (up to 8°C per base) and enhanced nuclease resistance. It's particularly valuable for ASO and diagnostic probe applications.
For long mRNA (over 200 nt), chemical synthesis is impractical. We offer a specialized Modified IVT Service that incorporates modified nucleosides such as pseudouridine and N1-methylpseudouridine during in vitro transcription. This enhances mRNA translational efficiency and reduces immunogenicity, as demonstrated in approved mRNA therapeutics.
HPLC purification is standard for all modified RNA orders. For complex sequences with multiple or closely-spaced modifications, we offer dual HPLC + PAGE purification. The mandatory HPLC step isolates the full-length product and removes truncated synthesis byproducts, which is critical for sensitive functional assays and therapeutic applications.

Ready to Start Your Project?

Get a customized quote for your Custom RNA modifications project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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