Trusted by Leading Research & Pharma Institutions

Livestock & Poultry Genome CRISPR Libraries

Accelerate your agricultural genomics research with genome-wide CRISPR knockout libraries specifically designed for livestock and poultry species. Our PigGeCKO and custom species libraries enable high-throughput functional gene screening for disease resistance, trait improvement, and breeding optimization.

Species-Specific

>98% Coverage

NGS Verified

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Trusted by leading research and pharmaceutical institutions

Cornell

Zoetis

UC Davis

Elanco

Wageningen

Boehringer

Why Choose Us

Species-specific genome annotation

>98% library coverage verified by NGS

4-6 sgRNAs per gene for high efficiency

Expert consultation included

Pig GeCKO Library

16,888 genes, 970,001 unique sgRNAs

Cattle Library

21,165 genes, 94,000 targeting guides

Custom Species

Sheep, goat, chicken, duck, and more

Coverage Rate

>98%

Service Overview

Species-Specific CRISPR Knockout Libraries for Agricultural Genomics

Our platform combines established CRISPR-Cas9 technology with species-specific genome annotations to deliver high-quality knockout libraries for livestock and poultry functional genomics research.

Pre-designed Species Libraries

We offer ready-to-use genome-wide CRISPR knockout libraries for major livestock species including pig (PigGeCKO v1.0), cattle (btCRISPRko.v1), sheep, and poultry. Each library is designed using the latest genome annotations for maximum coverage and accuracy.

Pig GeCKO: 16,888 genes, 970,001 sgRNAs
Cattle: 21,165 genes, 94,000 guides
Custom species upon request

Custom Library Design

Need a library for a different species or focused sub-library? Our expert team can design and construct custom CRISPR knockout libraries targeting specific gene sets, pathways, or genome regions for any agricultural species.

Any livestock or poultry species
Focused sub-libraries available
Pathway or gene family specific

Species-Specific Design

Optimized sgRNA design using latest genome annotations for each species.

NGS Quality Control

Every library undergoes next-generation sequencing verification with detailed reports.

Multiple Delivery Formats

Plasmid, virus, or cell pool - flexible formats for your screening needs.

Technology Platform

Advanced CRISPR Library Construction

Our proven technology platform ensures high-quality libraries with maximum coverage and minimal bias.

Optimized sgRNA Design

Our proprietary algorithms consider species-specific PAM sites, off-target potential, and codon usage to maximize knockout efficiency.

4-6 sgRNAs/gene Low Off-target

Massive Parallel Cloning

High-fidelity Gibson assembly or Golden Gate cloning ensures precise sgRNA integration with minimal bias during library construction.

Gibson Assembly Golden Gate

NGS Validation

Every library undergoes comprehensive NGS quality control to verify coverage, uniformity, and sgRNA representation.

>98% Coverage Uniformity QC

Quality Control

NGS Next-generation sequencing coverage verification

Sanger Preliminary validation of sgRNA representation

COA Certificate of Analysis included

QC Detailed sgRNA count and distribution report

Delivery Formats

Plasmid E. coli glycerol stock + plasmid DNA

Virus Lentivirus or AAV packaged library

Cell Pool Ready-to-screen cell pools available

Custom Additional formats available on request

Specifications

Library Specifications

Comprehensive specifications for our livestock and poultry CRISPR knockout libraries.

Parameter	Pig GeCKO Library	Cattle Library	Custom Libraries
Target Genes	16,888 genes	21,165 genes	Custom design
Total sgRNAs	970,001 unique	94,000 targeting	Custom design
sgRNAs per Gene	4-6 sgRNAs	4-5 sgRNAs	2-6 customizable
Species	Sus scrofa	Bos taurus	Any species
Coverage Rate	>99%	>98%	>98%
Vector System	Lentiviral (1-vector or 2-vector)	Lentiviral / PiggyBac	Customizable
Quality Verification	NGS Verified	NGS Verified	NGS Verified

Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

Library Design

Species-specific sgRNA design with optimized algorithms

Oligo Synthesis

High-fidelity oligo pool synthesis

Library Construction

Massive parallel cloning with Gibson assembly

NGS Validation

Comprehensive quality verification

Delivery

Secure packaging with full documentation

Applications

Diverse Applications in Agricultural Genomics

Our CRISPR knockout libraries support research and development across multiple fields.

Disease Resistance Breeding

Identify host genetic factors critical for pathogen replication to develop disease-resistant livestock. Our libraries have been used to screen for factors affecting JEV, ASFV, PRRSV, and other economically important pathogens.

Virus-host interaction studies
Host factor identification for major pathogens
Resistance gene discovery for marker-assisted selection
Anti-viral drug target identification

16,888+

Genes screened for disease resistance

Production Trait Improvement

Discover genes controlling economically important traits such as growth rate, feed efficiency, meat quality, and reproductive performance. Enable precision breeding through identified gene targets.

Growth and carcass trait genes
Feed conversion efficiency factors
Reproduction and fertility genes
Muscle development and meat quality

50+

Production traits investigated

Vaccine & Therapeutic Development

Support vaccine development by identifying critical viral dependencies and host entry factors. Our libraries enable systematic identification of intervention targets for novel vaccine and therapeutic strategies.

Viral entry receptor identification
Replication mechanism elucidation
Host-directed antiviral strategies
Diagnostic biomarker discovery

ASF, PRRS

Major pathogens targeted

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The PigGeCKO library exceeded our expectations. The coverage and uniformity were excellent, and the NGS report gave us confidence in our screening results. Identified several promising candidates for PRRSV resistance breeding."

Senior Scientist

Animal Health Research Institute

"Excellent customer support and technical expertise. They helped us design a custom library for our specific sheep breed and delivered on time with comprehensive documentation. Highly recommended for agricultural genomics projects."

Research Director

Livestock Breeding Company

"We've used multiple CRISPR library services, and this is by far the best for livestock species. The species-specific design optimization made a real difference in our screening efficiency. The team is responsive and understands our research needs."

Lead Researcher

University Agricultural Department

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

89 Citations

CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

Zhao C, Liu H, Xiao T, et al. Nature Communications. 2020.

First genome-scale CRISPR/Cas9 knockout library for pig (PigGeCKO v1.0) enabling high-throughput functional gene screening for disease resistance breeding.

View DOI

45 Citations

Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells

Yu C, Zhong H, Yang X, et al. Biotechnology Journal. 2022.

Comprehensive pig genome-scale CRISPR/Cas9 knockout library targeting 16,888 genes with 970,001 unique sgRNAs for functional genomics.

View DOI

32 Citations

GARP and EARP are required for efficient BoHV-1 replication as identified by a genome wide CRISPR knockout screen

Tan WS, Rong E, Dry I, Lillico SG, et al. PLoS Pathogens. 2023.

Development of btCRISPRko.v1 library targeting 21,165 cattle protein-coding genes for identifying bovine herpesvirus host dependencies.

View DOI

78 Citations

Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Vilela J, Rohaim MA, Munir M. Frontiers in Cellular and Infection Microbiology. 2020.

Comprehensive review of CRISPR/Cas9 applications in avian virology and poultry vaccine development.

View DOI

56 Citations

Advances in CRISPR-Based Functional Genomics and Nucleic Acid Detection in Pigs

Wang X, et al. Genes. 2022.

Review of CRISPR technologies for pig functional genomics, disease resistance breeding, and diagnostic applications.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our service.

We offer pre-designed genome-wide CRISPR knockout libraries for pig (Sus scrofa) and cattle (Bos taurus). Additionally, we can design and construct custom libraries for sheep, goat, chicken, duck, and other livestock/poultry species based on your specific requirements.

Every library undergoes comprehensive NGS (next-generation sequencing) quality control. We verify: (1) sgRNA representation and count, (2) coverage rate (>98%), (3) uniformity distribution, and (4) absence of bias. A detailed QC report with sequencing data is provided with each delivery.

We offer flexible delivery formats: (1) Plasmid DNA - E. coli glycerol stock plus purified plasmid, (2) Viral library - Lentiviral or AAV packaged libraries ready for cell transduction, (3) Cell pools - Cas9-expressing cells transduced with the library for immediate screening. Custom formats are available upon request.

Yes, we can design custom focused sub-libraries targeting specific gene sets, pathways, or genome regions. This is ideal when you have specific hypotheses or want to focus on particular biological processes. Our team will work with you to optimize the sgRNA design for your target genes.

We support multiple vector systems: (1) Lentiviral vectors - 1-vector (all-in-one) or 2-vector (Cas9 + sgRNA separate) systems, (2) PiggyBac transposon - for hard-to-transduce cells, (3) AAV vectors - for in vivo applications. The optimal system depends on your cell type and screening requirements.

Yes, we offer comprehensive CRISPR screening services including: (1) In vitro screening with various selection strategies, (2) In vivo screening in relevant models, (3) NGS deconvolution and bioinformatics analysis, (4) Hit validation and downstream characterization. Our experienced team can handle projects from library construction through final data analysis.

Ready to Start Your Project?

Get a customized quote for your Livestock and Poultry Genome CRISPR Knockout Libraries project. Our experts will respond within 24 hours.

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24h response

Expert consultation

Livestock & Poultry Genome CRISPR Libraries

Why Choose Us

Pig GeCKO Library

Cattle Library

Custom Species

Species-Specific CRISPR Knockout Libraries for Agricultural Genomics

Pre-designed Species Libraries

Custom Library Design

Species-Specific Design

NGS Quality Control

Multiple Delivery Formats

Ready to Accelerate Your Breeding Research?

Advanced CRISPR Library Construction

Optimized sgRNA Design

Massive Parallel Cloning

NGS Validation

Quality Control

Delivery Formats

Library Specifications

Streamlined Process from Design to Delivery

Library Design

Oligo Synthesis

Library Construction

NGS Validation

Delivery

Diverse Applications in Agricultural Genomics

Disease Resistance Breeding

Production Trait Improvement

Vaccine & Therapeutic Development

What Our Clients Say

Scientific Foundation

CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells

GARP and EARP are required for efficient BoHV-1 replication as identified by a genome wide CRISPR knockout screen

Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Advances in CRISPR-Based Functional Genomics and Nucleic Acid Detection in Pigs

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources

Request a Quote

Livestock & Poultry Genome CRISPR Libraries

Why Choose Us

Pig GeCKO Library

Cattle Library

Custom Species

Species-Specific CRISPR Knockout Libraries for Agricultural Genomics

Pre-designed Species Libraries

Custom Library Design

Species-Specific Design

NGS Quality Control

Multiple Delivery Formats

Ready to Accelerate Your Breeding Research?

Advanced CRISPR Library Construction

Optimized sgRNA Design

Massive Parallel Cloning

NGS Validation

Quality Control

Delivery Formats

Library Specifications

Streamlined Process from Design to Delivery

Library Design

Oligo Synthesis

Library Construction

NGS Validation

Delivery

Diverse Applications in Agricultural Genomics

Disease Resistance Breeding

Production Trait Improvement

Vaccine & Therapeutic Development

What Our Clients Say

Scientific Foundation

CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells

GARP and EARP are required for efficient BoHV-1 replication as identified by a genome wide CRISPR knockout screen

Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Advances in CRISPR-Based Functional Genomics and Nucleic Acid Detection in Pigs

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources