CD Biosynsis has established a superior synthetic biology platform to help our customers create scalable and efficient ways to produce keratinase. We are committed to developing effective synthetic biology strategies to overcome all the difficulties associated with traditional keratinase production and applications.
Advantages of Synthetic Biology-Driven Keratinase Production
Keratin is a stubborn fibrous protein found in large quantities in keratinous wastes such as feathers and hair. Keratinases are a class of proteases that can degrade keratin into amino acids. Different types of keratinases have different biochemical properties. They have various potential applications in multiple fields such as biomedicine, detergent, leather and textile industries, animal feeding, and biofertilizer production. So far, the traditional large-scale keratinase production process has faced great challenges in terms of enzyme yield, and it is urgent to find a method to improve the yield of keratinase. In addition, the biological yield of keratinase from natural keratinolytic hosts is low, and all of these problems greatly hinder the large-scale use of this enzyme. Emerging engineering strategies and advanced technological tools in synthetic biology allow the redesign and optimization of new biological pathways to construct engineered microbial strains for the efficient production of keratinase, which can boost the yield of keratinase.
Figure 1. General flow chart for the investigation of culturable keratinolytic microorganisms and their keratinases. (Daroit DJ, et al., 2014)
What We Provide
CD Biosynsis' advanced synthetic biology platform and extensive expertise allows us to help our customers develop customized and effective strategies to achieve improved production of keratinases.
Featured Services
- Isolation and screening of microorganisms with high keratinolytic activity as promising keratinase-producing microorganisms.
- Discovery and characterization of novel keratinases from novel keratinolytic microorganisms.
- Modification of keratinases for improved properties such as enhanced stability to high temperature and extreme pH, high catalytic efficiency, and increased tolerance to feedback inhibition.
- Improvement of keratinase production and activity by overexpressing highly efficient keratinases in native host strains or engineering of non-native hosts.
Key Strategies and Technologies
Deliverables
- Keratinase-producing microorganisms.
- High-quality keratinase products.
How We Can Help
CD Biosynsis offers our expertise and superior technical tools in the field of synthetic biology to help our customers achieve efficient production of keratinase in microorganisms. We are well positioned to help our customers solve the problems they may encounter in keratinase production and application.
Microbial Chassis Development for Keratinase Production
We can identify and select suitable host organisms and develop into synthetic biology chassis for keratinase production. We can provide engineered microbial strains with high keratinase productivity. The following table lists our collection of microbial strains that can be used for keratinase production. If you can't find the strain you are interested in, please fill out the online inquiry form and tell us more about your project.
| Kocuria rosea |
Scopulariopsis brevicaulis |
Aspergillus oryzae |
Bacillus licheniformis |
Doratomyces microsporum |
| Stenotrophomonas sp. |
Streptomyces aureofaciens |
Aspergillus parasiticus |
Bacillus polymyxa |
Microsporum gypseum |
| Microsporum canis |
Trichophyton rubrum |
Bacillus cereus |
Bacillus pumilus |
Paecilomyces marquandii |
Development of Heterologous Expression Systems for Keratinase Production
With our expertise in microbial synthetic biology, metabolic engineering, genetic engineering, and enzyme engineering, we can reconstruct microbial biosynthetic pathways for heterologous production of keratinases in microorganisms such as Escherichia coli and Pichia pastoris. We can optimize the metabolic pathways to reduce by-product formation and improve the production performance of keratinase-producing microorganisms. Our experienced scientists can help our customers choose the optimal route for heterologous keratinase production and construct cost-effective microbial cell factories.
Applications of Keratinases
CD Biosynsis offers customized solutions for synthetic biology-driven keratinase production to meet its growing needs in a wide range of fields such as the pharmaceutical, cosmetic, and leather industries.
- Used in wastewater treatment and removal of obstructions in sewage systems.
- Used in the development of hair removal products.
- Used in the treatment of acne and callus removal.
- Used in the treatment of nail disorders.
- Used in the extraction of keratin to produce biomaterials for biomedical applications.
- Used in the leather industry for hair removal treatments.
- Used as a detergent additive to remove protein stains.
Want to Learn More?
As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for industrial enzyme production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.
References
- Vidmar B; Vodovnik M. Microbial Keratinases: Enzymes with Promising Biotechnological Applications. Food Technol Biotechnol, 2018, 56(3):312-328.
- Daroit DJ; Brandelli A. A current assessment on the production of bacterial keratinases. Crit Rev Biotechnol, 2014, 34(4):372-84.
- Yahaya RSR, et al. Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications. Appl Microbiol Biotechnol, 2021, 105(10):3955-3969.
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