Introduction
The use of antibiotic resistance genes as selection markers in synthetic biology strains has been a common practice for many years. However, the increasing concern over the spread of antibiotic resistance has prompted scientists to develop alternative selection systems. The use of antibiotic-free selection systems is not only a safer option but also more cost-effective in the long run.
CD Biosynsis recognizes the importance of antibiotic resistance marker removal in synthetic biology strains, and offers a range of services to assist researchers in this field. Our team of experts has extensive experience in the development and optimization of alternative selection systems, and we use the latest technologies and techniques to ensure the highest quality results.
Figure 1: Elimination of the apramycin resistance gene in the host strain genome. (Sevillano, L., et al. 2017)
Our Services for Antibiotic Resistance Marker Removal
Customized Marker Removal Services
we offer customized marker removal services tailored to the specific needs of each client. Our team of experts will work closely with you to design and optimize a marker removal strategy that meets your research goals and timelines.
CRISPR/Cas9-mediated Marker Removal
CRISPR/Cas9 technology has revolutionized the field of genetic engineering, providing a precise and efficient method for targeted gene modification. CD Biosynsis utilizes CRISPR/Cas9 technology to remove antibiotic resistance marker genes from synthetic biology strains, enabling the production of genetically modified organisms without the use of antibiotics.
Cre/LoxP Recombination-mediated Marker Removal
Cre/LoxP recombination is a well-established genetic engineering technique that enables the precise deletion of specific DNA sequences. CD Biosynsis utilizes Cre/LoxP recombination to remove antibiotic resistance marker genes from synthetic biology strains, providing a reliable and efficient method for genetic modification.
Flp/FRT Recombination-mediated Marker Removal
Flp/FRT recombination is another well-established genetic engineering technique that enables the precise deletion of specific DNA sequences. CD Biosynsis utilizes Flp/FRT recombination to remove antibiotic resistance marker genes from synthetic biology strains, providing a versatile and efficient method for genetic modification.
Our Advantages
- Safer and More Sustainable Genetic Engineering
- Elimination of antibiotic resistance genes reduces the risk of horizontal gene transfer and promotes safer genetic engineering practices.
- Our services provide a sustainable solution for genetic engineering by reducing the use of antibiotics.
- Precise and Efficient Marker Removal
- Our team of experts utilizes cutting-edge genetic engineering techniques to ensure the precision and efficiency of the marker removal process.
- The use of techniques such as CRISPR/Cas9, Cre/LoxP recombination, and Flp/FRT recombination ensures the precise deletion of specific DNA sequences.
- Customized Marker Removal Services
- Our services are tailored to the specific needs of each client, providing a flexible and versatile solution for genetic engineering.
- We work closely with clients to design and optimize a marker removal strategy that meets their research goals and timelines.
- Validation of Marker Removal Efficacy
- We validate the efficacy of marker removal using a range of techniques, ensuring the final strains are free of antibiotic resistance genes and safe for research.
- Our validation process includes gene expression analysis, protein production, and functional assays.
CD Biosynsis is committed to providing our clients with the highest quality services in antibiotic resistance marker removal in synthetic biology strains, using the latest technologies and techniques to ensure the safety and efficacy of your research. Contact us today to learn more about our Antibiotic Resistance Marker Removal services and how we can assist you in your research.
References
- Sevillano, L., et al. Development of an antibiotic marker-free platform for heterologous protein production in Streptomyces. Microbial Cell Factories 16, 164 (2017).
- Iamtham, S., Day, A. Removal of antibiotic resistance genes from transgenic tobacco plastids. Nature Biotechnology 18, 1172–1176 (2000).
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