Synthetic Biology
Strain Engineering for Lactic Acid

Strain Engineering for Lactic Acid

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Strain Engineering for Lactic Acid

CD Biosynsis has developed advanced synthetic biology tools to engineer microorganisms for efficient fermentative production of lactic acid (LA) to meet the growing demand for LA across a wide variety of industries, from food to pharmaceutical industries. Our scientists with extensive knowledge and expertise in synthetic biology and fermentation have the ability to help our customers significantly improve LA production and achieve green lactic acid production from renewable resources.

Advantages of Synthetic Biology-Driven Lactic Acid Production

Lactic acid or lactate, with the molecular formula C3H6O3, is a naturally occurring organic acid found in microorganisms, plants, and animals. The global demand for LA has been constantly increasing due to its wide range of applications in various industries and the discovery of new applications every year. Industrially, LA can be produced by chemical synthesis or microbial fermentation of carbohydrates. The chemical synthesis method suffers from environmental pollution, highly expensive, and low optical purity, while the fermentative production of LA offers several advantages such as low cost, relatively simple process, and eco-friendly. The unprecedented capabilities of synthetic biology to engineer microorganisms for fermentative production of LA will greatly help meet the growing market demand for LA in the near future.

Figure 1. Frequency of synthetic biology patents related to cellulosic ethanol. Source: Research data from Questel-orbit platform. (Fantinel A L, et al., 2021)Figure 1. A schematic representation of the two manufacturing processes of lactic acid. (Ghaffar T, et al., 2014)

What We Provide

Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of lactic acid.

Featured Services

  • Metabolic engineering of lactic acid-producing microorganisms to increase the lactic acid yield and productivity.
  • Identification and engineering of key enzymes for glycolytic and lactate fluxes.
  • Rerouting of pyruvate metabolism or enhancing the expression of lactate dehydrogenase to achieve high yields.
  • Design and engineering new pathways in non-natural host strains to improve their lactic acid production capability.


  • Lactic acid-producing microorganisms.
  • Optically pure L-lactic acid and D-lactic acid

How We Can Help

CD Biosynsis’ expertise in bacterial synthetic biology, fungal synthetic biology and algae synthetic biology allows us to help our customers deal with the engineering of microbial cells and microbial consortia to develop robust and highly efficient microbial LA producers to improve fermentative production of LA using renewable resources as substrates.

Engineering of Microbial Chassis for Improved Lactic Acid Production

Our scientists can help our customers select the suitable microorganisms from our own microbial strain bank and develop them into powerful synthetic biology chassis with improved LA productivity. We have developed a wide collection of synthetic biology tools and approaches for efficient engineering of bacteria, fungi, and algae. We focus on the pathways of lactic acid production using substrates including carbohydrates, food wastes, diary wastes, industrial waste, and algal biomass in the following microbials. If you are interested in other strains or substrates, please fill out the online inquiry form and tell us more about your project.

Lactobacillus amylovorus Lactobacillus bulgaricus Lactobacillus casei Lactobacillus coryniformis Lactobacillus delbrueckii
Lactobacillus helveticus Lactobacillus pentosus Lactobacillus plantarum Lactobacillus rhamnosus Lactobacillus salivarius
Lactobacillus uvarum Lactobacillus zeae Lactococcus lactis Streptococcus thermophilus Enterococcus faecalis
Enterococcus faecium Bacillus coagulans Bacillus subtilis Corynebacterium glutamicum Escherichia coli
Enterococcus mundtii Sporolactobacillus inulinus Rhizopus oryzae Kluyveromyces marxianus Monascus ruber
Saccharomyces cerevisiae Pichia stipitis Candida utilis Schizosaccharomyces pombe Synechocystis sp.

Synthetic Biology Strategies for Overcoming Challenges in Lactic Acid Production

Our scientists who have been working on synthetic biology and precision fermentation for many years can help our customers develop optimized strategies to overcome major challenges in LA production, including LA tolerance and productivity, isomer purity, nutrient requirements, substrate inhibition, end product inhibition, and high-cost substrates. We are committed to leveraging the power of synthetic biology to advance the sustainable lactic acid production from renewable substrates.

Applications of Lactic Acid

CD Biosynsis can provide our customers with customized solutions for synthetic biology-driven lactic acid production to meet the increasing demand in the following industries.

  • Used as natural additives, flavoring agents, food preservative agents, fermentation agents, acidulants, pH regulators, antioxidants, decontaminants, cryoprotectants, and viscosifiers in the food industry.
  • Used as building-blocks in the synthesis of various chemicals such as polylactic acid (PLA), ethyl lactate, lactide, lactate esters, and acrylic acid
  • Used as descaling agents, neutralizers, green solvents, chiral intermediates, and cleaning agents in the chemical industry.
  • Used as skin rejuvenating & whitening agents, humectants, moisturizers, and anti-tartar agents in the cosmetic industry.
  • Used in dialysis solutions, surgical sutures, mineral preparations, controlled drug delivery systems, probiotic supplements in the medical and pharmaceutical industry.
  • Used as mordants in the textile industry.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.


  1. Ghaffar T, et al. Recent trends in lactic acid biotechnology: a brief review on production to purification. Journal of radiation research and applied Sciences, 2014, 7(2): 222-229.
  2. Jullesson D, et al. Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals. Biotechnology advances, 2015, 33(7): 1395-1402.

Production of Acetic Acid from Algae

Our scientists with expertise in algae synthetic biology are working to explore the possibility of producing acetic acid from microalgae such as Chlorella sp. and Tetraselmis suecica. We expect to develop a promising process for acetic acid production using microalgae biomass as feedstock. Our dedicated scientists also aim to develop a sustainable and eco-friendly process for the production of acetic acid from algae.

Please note that all services are for research use only. Not intended for any clinical use.

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