Saccharomyces cerevisiae Genetic Engineering Services

Overview

Saccharomyces cerevisiae, commonly known as baker's yeast, is a model organism extensively used in genetics, molecular biology, and biotechnology. Its ease of genetic manipulation and well-characterized genome make it an ideal system for various applications. Our services leverage advanced genetic engineering techniques, including CRISPR/Cas9, homologous recombination, and plasmid-based transformations, to deliver high-quality, custom-engineered yeast strains.

The experimental method process generally includes the following steps:

1. Determine target genes: Firstly, identify the target genes that need to be edited or modified, and consider the desired functions or traits when determining the target genes.
2. Design editing tools: Use gene editing tools such as CRISPR/Cas9 to design suitable primers or RNA sequences for targeted editing or modification of target genes.
3. Transform DNA: Transform editing tools and target genes into yeast together. This step can be achieved through methods such as transfection, electroporation, and flagella.
4. Select transformation colonies: After transformation, the yeast colonies that have been successfully transformed should be screened through selective culture media or fluorescence screening methods.
5. Confirm editing effect: Use molecular biology methods such as PCR and Southern blotting to verify the editing effect of the target gene.
6. Identification of genetically modified strains: Identification of edited or modified yeast strains through sequencing and other methods.
7. Perform functional analysis: Perform functional analysis on edited or modified genetically modified yeast to verify whether the function or traits of the target gene have been altered.

Through these steps, genetic engineering of Saccharomyces cerevisiae can be achieved, thereby modifying or utilizing the characteristics of the yeast.