DNA Construction Excellence

Synthetic Biology Build Services

Transform your designs into reality with our precision DNA synthesis and assembly services. From single genes to complex pathways, our automated platforms deliver high-quality constructs for your synthetic biology research with rapid turnaround.

Gene Synthesis

DNA Assembly

Quality Control

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Trusted by leading research and pharmaceutical institutions

Research Universities

Pharma Companies

BioTech Startups

Industrial Biotech

Build Capabilities

Gene synthesis up to 20kb

Golden Gate assembly

Gibson Assembly services

Full sequence verification

Multi-Part Assembly

Complex pathway construction

Sequence Verification

NGS-based quality control

Strain Development

Bacterial and yeast strains

Service Overview

Precision DNA Construction

Our Build services transform computational designs into physical DNA constructs with industry-leading accuracy and quality standards.

Gene Synthesis

High-fidelity gene synthesis with automatic codon optimization. Sequences up to 20kb with guaranteed accuracy and full sequence verification.

Automatic codon optimization
Sequence verification included
Expression vector cloning
Multiple delivery formats

Golden Gate Assembly

Seamless multi-part assembly using Type IIS restriction enzymes. Perfect for modular construct design with scarless joins.

MoClo and Level 0 systems
Scarless assembly
Up to 20 parts in one reaction
Standardized part libraries

Gibson Assembly

Sequence-independent assembly for complex constructs. Ideal for pathway assembly, vector construction, and fragment joining.

Overlap-based assembly
Up to 15 fragments
No restriction enzymes required
High efficiency transformation

Quality Control

Comprehensive sequence verification using NGS technology. Every construct verified before delivery with detailed quality reports.

NGS-based verification
Variant detection
Clonal isolation
Complete documentation

Technology

Advanced Construction Platforms

State-of-the-art DNA synthesis and assembly technologies delivering high-quality constructs with industry-leading accuracy.

High-Fidelity Synthesis

Advanced synthesis chemistry with integrated error correction ensures the highest accuracy gene synthesis available. Automatic sequence verification on every construct.

99.9% Accuracy Error Correction Up to 20kb

Golden Gate Assembly

Proprietary optimized assembly reactions enable highly efficient multi-part joining. Our Golden Gate platform handles complex assemblies with consistent success rates.

Type IIS Enzymes Scarless Joining High Efficiency

Gibson Assembly

Optimized Gibson Assembly chemistry for seamless fragment joining without restriction enzymes. Perfect for complex pathway construction and vector engineering.

Sequence-Independent No scars Multi-Fragment

NGS Quality Control

Next-generation sequencing verification ensures complete accuracy on every construct. Detects variants, contamination, and assembly errors before delivery.

100x Coverage Variant Detection Complete Report

Technology Comparison

Method	Fragments	Scarless
Golden Gate	Up to 20	Yes
Gibson	Up to 15	Yes
SLIC	Up to 10	Yes
Yeast Assembly	Up to 10	Yes

We optimize assembly conditions for each project to maximize success rates and ensure construct integrity.

Specifications

Build Service Parameters

Comprehensive DNA construction capabilities with flexible configuration options.

Gene Synthesis

Sequence length: 0.3kb to 20kb
Automatic codon optimization
Multiple delivery vectors
Expression system options
Fluorescent tag options

Golden Gate Assembly

MoClo-compatible format
Up to 20 parts per reaction
Scarless assembly
Level 0 to Level 3 support
Standard parts library access

Gibson Assembly

Up to 15 fragment assembly
Sequence-independent joining
No restriction sites required
Customizable overlap design
High transformation efficiency

Quality Control

NGS verification included
100x sequencing coverage
Variant detection
Complete sequence confirmation
Detailed QC report

Strain Development

E. coli strain construction
Yeast strain development
Clonal isolation included
glycerol stock delivery
Sequence-verified clones

Delivery Options

Plasmid DNA delivery
Linear DNA fragments
Bacterial strain shipment
Multiple clone formats
Custom documentation

Custom Assembly Services

Don't see your assembly method? Contact us for custom assembly services including yeast assembly, PCR-based methods, and proprietary protocols.

Workflow

Our Build Process

A systematic approach to DNA construction ensuring accuracy and quality at every step.

Design Review

Sequence analysis and construct review. We verify design feasibility, optimize assembly strategy, and identify any potential issues.

Sequence analysis
Assembly planning
Strategy optimization

DNA Synthesis

High-fidelity oligonucleotide synthesis and gene assembly. Built-in error correction ensures industry-leading accuracy.

Oligo synthesis
Error correction
Quality verification

Assembly & Cloning

Multi-fragment assembly using optimized Golden Gate or Gibson methods. High-efficiency transformation and clonal isolation.

Assembly optimization
Transformation
Colony screening

Verification & Delivery

NGS-based sequence verification ensures 100% accuracy. Complete documentation and quality report with every delivery.

NGS verification
Quality report
Secure delivery

Applications

Build Service Use Cases

Our DNA construction services support diverse synthetic biology and molecular biology applications.

Metabolic Engineering

Construct complex metabolic pathways for production of fuels, chemicals, and pharmaceuticals. Multi-gene assembly for pathway construction.

Biofuels Fine Chemicals Pharmaceuticals

Genome Engineering

Build CRISPR constructs, knock-in donors, and genome editing tools. High-fidelity constructs for precise genetic modifications.

CRISPR Knock-in Base Editing

Gene Expression

Construct expression vectors for protein production in bacterial, yeast, and mammalian systems. Optimized for high yield.

E. coli Yeast CHO

Genetic Circuits

Build synthetic gene circuits for research applications. Modular parts for logic gates, oscillators, and feedback systems.

Logic Gates Biosensors Memory

Therapeutic Development

Construct DNA for gene therapy vectors, vaccine development, and cell engineering applications. GMP-quality constructs available.

Gene Therapy Vaccines Cell Therapy

Protein Engineering

Build expression constructs for protein production, enzyme engineering, and structural biology. Multiple tag options available.

Enzymes Antibodies Fusion Proteins

Testimonials

What Our Clients Say

Trusted by researchers and companies worldwide.

"The gene synthesis accuracy is outstanding. We've ordered dozens of constructs and every single one has matched the design perfectly. The NGS verification gives us confidence."

Research Scientist

Academic Laboratory

"The Golden Gate assembly service made constructing our 8-gene pathway effortless. The scarless assembly and standardized format saved us months of work."

Principal Investigator

Metabolic Engineering Lab

"For our CRISPR project, we needed hundreds of guide RNA constructs. The fast turnaround and consistent quality made this possible. Highly recommended."

Senior Scientist

Pharmaceutical Company

FAQ

Frequently Asked Questions

Find answers to common questions about our DNA construction services.

What sequence lengths can you synthesize?

We offer gene synthesis from 0.3kb to 20kb. For constructs larger than 20kb, we recommend our pathway assembly services which can combine multiple synthesized fragments. Contact us for very large construct requirements.

What is the sequence accuracy guarantee?

All constructs undergo NGS verification with 100x coverage. We guarantee 100% sequence accuracy for the delivered construct. If any errors are detected, we will resynthesize the construct at no additional cost.

Do you offer codon optimization?

Yes, automatic codon optimization is included with all gene synthesis orders. We optimize for your specified expression host while avoiding rare codons, sequence repeats, and problematic structures. You can also provide your own optimized sequence.

What assembly methods do you support?

We support Golden Gate assembly (MoClo-compatible), Gibson Assembly, SLIC, and yeast assembly. Golden Gate is ideal for modular, scarless assembly with standardized parts. Gibson is better for sequence-independent multi-fragment assembly.

How many fragments can you assemble?

Golden Gate assembly supports up to 20 parts in a single reaction. Gibson Assembly can handle up to 15 fragments. For larger assemblies, we use iterative assembly strategies that have been validated for pathways up to 50kb.

What expression hosts are supported?

We support expression in E. coli, Bacillus subtilis, Saccharomyces cerevisiae, Pichia pastoris, and CHO cells. We can also clone into custom vectors. Codon optimization is host-specific for optimal expression.

What delivery formats are available?

Standard delivery is purified plasmid DNA in TE buffer. We also offer bacterial strain glycerol stocks, linear DNA fragments, and virus vector DNA. Custom formats and packaging are available upon request.

Do you offer strain development services?

Yes, we offer complete strain development including plasmid construction, transformation, clonal isolation, and sequence verification. E. coli and yeast strain development are available. We can also provide multiple independent clones for your project.

Ready to Start Your Project?

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24h response

Expert consultation

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Synthetic Biology Build Services

Build Capabilities

Multi-Part Assembly

Sequence Verification

Strain Development

Precision DNA Construction

Gene Synthesis

Golden Gate Assembly

Gibson Assembly

Quality Control

Ready to Build Your Constructs?

Advanced Construction Platforms

High-Fidelity Synthesis

Golden Gate Assembly

Gibson Assembly

NGS Quality Control

Technology Comparison

Build Service Parameters

Gene Synthesis

Golden Gate Assembly

Gibson Assembly

Quality Control

Strain Development

Delivery Options

Custom Assembly Services

Our Build Process

Design Review

DNA Synthesis

Assembly & Cloning

Verification & Delivery

Build Service Use Cases

Metabolic Engineering

Genome Engineering

Gene Expression

Genetic Circuits

Therapeutic Development

Protein Engineering

What Our Clients Say

Frequently Asked Questions

What sequence lengths can you synthesize?

What is the sequence accuracy guarantee?

Do you offer codon optimization?

What assembly methods do you support?

How many fragments can you assemble?

What expression hosts are supported?

What delivery formats are available?

Do you offer strain development services?

Ready to Start Your Project?

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