CD Biosynsis is committed to reducing the cost of itaconic acid production and increasing the yield of itaconic acid by regulating gene expression, eliminating by-products, and enhancing the strain's resistance to adversity.
Itaconic acid, also known as methylene dibutyric acid, is considered to be the cleanest alternative to petroleum-based acrylic acid. Itaconic acid can be synthesized chemically or biosynthetically. However, the chemical synthesis of itaconic acid requires multiple stages and low production efficiency, so chemical synthesis has never been used for commercial production of itaconic acid. Microbial fermentation is the most commonly used method for itaconic acid production, mainly obtained by fermentation using filamentous fungi. With the development of genetic engineering, metabolic engineering, and other technologies, synthetic biology-driven itaconic acid production is not only high yielding but also easy to control and improve.
Figure 1. Schematic representation of life cycle of rapidly renewable itaconic acid. (Bafana R, et al., 2018)
Our experienced scientists are dedicated to helping our customers solve problems in the production of itaconic acid using advanced technology. We want to provide you with high-quality and efficient one-stop service.
We expect to use metabolic engineering tools to help our customers improve the productivity of microorganisms for itaconic acid, reduce the productivity of by-products, and develop strains that can produce itaconic acid from inexpensive raw materials.
We are able to use genetic engineering tools to increase the productivity of natural producers of itaconic acid and also to modify non-natural producing strains to have the ability to produce itaconic acid.
The cellular morphology of microbial chassis may affect the production of itaconic acid. We are able to use morphological engineering tools to improve the production of itaconic acid.
We are able to find key enzymes affecting the microbial itaconic acid synthesis pathway through transcriptome analysis and establish optimal parameters for itaconic acid biosynthesis.
Development of Synthetic Biology Chassis for Itaconic Acid Production
Many microorganisms use cis-aconitate as a substrate to produce itaconic acid outside the mitochondria via cis-aconitate decarboxylase. The following microbial chassis have been successfully implemented for the production of itaconic acid. If you would like to learn about other microbial chassis development services, please contact us directly.
|Aspergillus terreus||Ustilago zeae||Ustilago maydis|
|Candida sp.||Pseudozyma antarctica||Rhodotorula species|
|Ustilago maydis||Yarrowia lipolytica|
Itaconic Acid Producing Strain Improvement
Natural itaconic acid-producing strains have clusters of genes that specifically encode secretase and transporter proteins involved in itaconic acid production. We are able to use genetic engineering tools to regulate the expression of this gene cluster for efficient accumulation of itaconic acid production. We are able to provide the following strain improvement services, including but not limited to.
Multi-Enzyme Self-Assembly for the Improvement of Itaconic Acid Production
Multi-enzyme cascade reactions can synthesize simple compounds with low cost into complex compounds with high added value through multi-step enzymatic reactions. We are able to use multi-enzyme self-assembly technology to achieve the self-assembly of multiple enzymes by intracellular co-expression of fusion proteins to improve the yield of itaconic acid.
Optobiochemical Control of Protein Activities
We are able to design and assemble light-sensing structural domains into the microbial photoregulatory system to control the function of proteins through light. We are able to help our customers establish optimal light control strategies to improve the production of itaconic acid.
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive itaconic acid production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in itaconic acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.