CD Biosynsis offers comprehensive Strain Genetic Engineering Services dedicated to designing, constructing, and optimizing microbial cell factories for the enhanced production of target compounds, including biopharmaceuticals, industrial enzymes, and renewable chemicals. We utilize a powerful toolkit of precise genomic editing technologies, such as CRISPR-Cas systems, TALENs, and advanced homologous recombination, combined with high-throughput screening and metabolic modeling. Our expertise spans a wide range of hosts, including Escherichia coli, Saccharomyces cerevisiae, and various specialized strains. By rationally engineering microbial genomes, we can introduce heterologous pathways, eliminate competing side reactions, improve tolerance to stress, and boost overall product titer and yield, ensuring efficient and commercially viable biomanufacturing processes.
Get a QuoteStrain genetic engineering is the foundation of industrial biotechnology, enabling the creation of custom organisms capable of efficient biosynthesis. Our approach is based on the Design-Build-Test-Learn (DBTL) cycle. We begin with rational design, leveraging metabolic flux analysis and computational tools to pinpoint genetic modification targets. The Build phase utilizes state-of-the-art editing techniques for precise genomic integration and deletion. The Test phase employs high-throughput fermentation and analytical chemistry to assess performance. Finally, the Learn phase uses high-dimensional data analysis to inform the next round of design. This iterative, systematic method allows us to quickly navigate the complex genetic landscape of host organisms to achieve superior performance metrics, such as increased product titer, yield, and purity.
Check the box next to your desired host organism:
Escherichia coli (E. coli)
Saccharomyces cerevisiae (Yeast)
Bacillus Species
Filamentous Fungi
Pichia pastoris
Specialized Actinomycetes
Cyanobacteria/Algae
Custom/Proprietary Strains
Select the genetic engineering approach for your project:
Pathway Heterologous Integration
Gene Knockout (Eliminating Byproducts)
Promoter/Terminator Library Screening
Codon Optimization & T7 Expression
rRNA/tRNA Gene Modification
CRISPR-Mediated Multiplex Editing
Adaptive Laboratory Evolution (ALE)
Gene Point Mutation/Saturation Mutagenesis
Genomic Sequencing
Verification of all planned genomic edits, integrations, and deletions via whole-genome sequencing (WGS) or targeted sequencing.
Metabolomics/Flux Analysis
Measurement of intracellular metabolite levels and carbon flux redistribution to validate pathway function and identify bottlenecks.
Bioreactor Validation
Testing the engineered strain performance (Titer, Yield, Productivity) in fed-batch or continuous bioreactor trials.
A systematic and iterative cycle for developing high-performance microbial strains.
Design (Target Identification)
Build (Genetic Construction)
Test (Performance Verification)
Learn (Data Analysis & Iteration)
Target Pathway Mapping: Define the biosynthetic route for the target molecule and select the optimal host organism.
Computational Modeling: Use metabolic models to predict the impact of gene modifications (knockouts, overexpression) on flux.
Genomic Modification: Utilize CRISPR-Cas or Recombineering for precise insertion or deletion of target genes and pathways.
Pathway Assembly: Synthesize and assemble complex genetic circuits (e.g., biosynthetic gene clusters) into the host genome.
High-Throughput Screening: Screen hundreds to thousands of engineered strains using automated systems (e.g., microplate readers, flow cytometry).
Fermentation Analysis: Validate top performers in lab-scale bioreactors to determine Titer, Yield, and Productivity (TYP).
Multiplexing & High Precision
Ability to perform multiple, simultaneous, and precise genomic edits (insertions, deletions) using advanced CRISPR tools.
Broad Host Compatibility
Proven experience engineering diverse industrial hosts, from bacteria and yeast to filamentous fungi and algae.
Metabolic Pathway Integration
Seamless and stable insertion of large, complex heterologous biosynthetic pathways into the host genome.
Optimized TYP
Final strains are optimized not just for Titer but also for Yield and Productivity, crucial metrics for industrial scale-up.
"The multiplex CRISPR engineering eliminated three competitive pathways in our E. coli strain, resulting in a 45% increase in the yield of our target amino acid."
Dr. Lena Fischer, Industrial Fermentation Lead
"CD Biosynsis successfully integrated a complex 12-gene polyketide pathway into our proprietary yeast host, delivering a stable and high-titer producer ready for pilot scale."
Mr. Kenji Tanaka, Natural Product Discovery
"The WGS validation and metabolomics data provided gave us the confidence needed to move our engineered Bacillus strain directly into large-scale commercial production."
Ms. Olivia Chen, Biomanufacturing Director
What is the key goal of Strain Genetic Engineering?
The primary goal is to transform a standard microorganism into a high-performance microbial cell factory. This is achieved by engineering the strain to efficiently convert low-cost raw materials into a high concentration (titer) of a specific target molecule with minimal undesirable byproducts (high yield).
Which genetic editing tools do you primarily use?
We routinely use advanced editing tools including CRISPR-Cas9/CRISPR-Cpf1 for precise, multiplex editing; Recombineering (recA/red/gam systems) for efficient homologous recombination in E. coli; and specialized vector systems for difficult-to-transform hosts like Pichia and filamentous fungi.
How do you ensure the stability of the engineered pathway?
We prioritize stable genomic integration over plasmid-based expression wherever possible. Furthermore, we optimize codon usage and select robust, constitutive or highly regulated promoters to ensure that the heterologous pathway genes are expressed stably and consistently under industrial fermentation conditions.
What is Adaptive Laboratory Evolution (ALE)?
ALE is a method where a strain is grown under selective pressure (e.g., high product concentration, high temperature) for hundreds of generations. This naturally selects for beneficial mutations that improve strain fitness and performance under those specific industrial conditions, and can be used to complement rational genetic engineering.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.