Trusted by Leading Research & Pharma Institutions

Diagnostic Probes Oligos Synthesis

Precision-manufactured fluorescent oligonucleotide probes for molecular diagnostics, qPCR, and genomic detection applications. Our proprietary synthesis platform delivers exceptional coupling efficiency with comprehensive quality verification for clinical and research applications.

ISO 13485 Certified

99.4% Coupling

ESI-MS Verified

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Trusted by leading research and pharmaceutical institutions

Johns Hopkins

Roche

MIT

Pfizer

Stanford

Novartis

Why Choose Us

Better coupling efficiency than competitors

Complete QC documentation

Multiplex probe solutions

ISO 13485 certified manufacturing

qPCR Probes

TaqMan, dual-quencher, MGB/LNA modified

FISH Probes

Multi-fluorophore labeling for genomic detection

Molecular Diagnostics

Pathogen detection, SNP genotyping

Quality Grade

ISO 13485

Service Overview

Precision Diagnostic Probe Synthesis

Advanced oligonucleotide synthesis for molecular diagnostics with superior coupling efficiency and comprehensive quality control.

Proprietary Synthesis Platform

Our vertically-integrated synthesis platform delivers better coupling efficiency than competitors, with 99.4% efficiency compared to industry average of 98.5%. Every component from reagents to synthesizers is engineered in-house for optimal performance.

99.4% coupling efficiency (vs 98.5% competitors)
In-house reagent formulation
Custom engineered synthesizers

Comprehensive Quality Control

Every diagnostic probe undergoes rigorous ESI mass spectrometry verification to confirm exact sequence and purity. Complete batch records and Certificate of Analysis ensure regulatory compliance for diagnostic applications.

ESI-MS verification on every probe
Complete traceability documentation
ISO 13485 certified manufacturing

qPCR Probes

TaqMan probes, dual-quencher, MGB, LNA modified for SNP genotyping.

FISH Probes

Multi-fluorophore labeling with high brightness for genomic detection.

Multiplex Solutions

Up to 6-plex capability with validated fluorophore combinations.

Technology Platform

Advanced Probe Synthesis Technologies

State-of-the-art synthesis and modification technologies for diagnostic applications.

Phosphoramidite Chemistry

Optimized phosphoramidite chemistry with 99.4% coupling efficiency, delivering more full-length product than competitors at 98.5%.

99.4% Efficiency Proprietary

Advanced QC Systems

Every probe undergoes ESI mass spectrometry verification. Complete batch records ensure regulatory compliance for diagnostic applications.

ESI-MS CE Analysis

Dual-Quencher Technology

Proprietary dual-quencher probes with internal quenchers for improved signal-to-noise ratio compared to traditional single-quencher designs.

ZEN TAO

Fluorophore & Quencher Options

Dyes FAM, HEX, TET, JOE, Cy3, Cy5, Cy5.5, ROX, TAMRA

Quenchers BHQ1, BHQ2, DABCYL, Iowa Black FQ/RQ

Dual ZEN/Iowa Black FQ, TAO quenchers

Specialized Modifications

MGB Minor groove binder for SNP discrimination

LNA Locked nucleic acid for enhanced Tm

PS Phosphorothioate for nuclease resistance

Parameter	qPCR Probes	FISH Probes	Diagnostic Grade
Length Range	10 - 60 nt	40 - 120 nt	10 - 100 nt
Coupling Efficiency	99.4%	99.4%	99.4%
Purification	HPLC, PAGE	HPLC, PAGE	HPLC, dual purification
Scale Range	0.025 - 10 μmol	0.1 - 5 μmol	0.5 - 50 μmol
QC Verification	ESI-MS, CE	ESI-MS, UV	ESI-MS, CE, COA
Quality Certification	ISO 9001	ISO 9001	ISO 13485

Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

Sequence Design

Submit probe sequences and modification specifications

Synthesis

Proprietary platform synthesis with 99.4% efficiency

QC Verification

ESI-MS and CE purity analysis

Documentation

Batch records and Certificate of Analysis

Delivery

Quality-verified probes with full documentation

Applications

Diverse Applications Across Diagnostics

Our synthesis services support molecular diagnostics research and development.

Real-Time PCR Detection

High-quality fluorescent probes for real-time PCR applications including gene expression analysis, pathogen detection, and copy number variation studies.

TaqMan hydrolysis probes
Dual-quencher for improved signal-to-noise
Multiplex up to 6 targets
Validated fluorophore combinations

99.4%

Coupling efficiency

Infectious Disease Detection

Validated probe sequences and custom synthesis for pathogen detection including viral, bacterial, and fungal targets for diagnostic assay development.

Viral RNA detection probes
Bacterial 16S rRNA probes
Multiplex respiratory panels
GMP grade available

6-Plex

Maximum multiplexing

SNP Genotyping

Specialized probe chemistries including MGB and LNA modifications for enhanced single nucleotide polymorphism discrimination.

MGB Eclipse probes for SNP
LNA-modified affinity probes
Allele-specific discrimination
Clinical sample compatible

1 bp

Single-base discrimination

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The coupling efficiency is noticeably better than our previous supplier. We're getting more full-length product and better signal-to-noise in our qPCR assays. The ESI-MS verification gives us confidence in every order."

Senior Scientist

Molecular Diagnostics Company

"The dual-quencher probes have significantly improved our multiplex assay performance. The complete documentation package made regulatory submissions much smoother. Excellent customer support."

Research Director

IVD Development Company

"We've been using their FISH probes for genomic detection. The multi-fluorophore labeling quality is excellent. Batch-to-batch consistency has been critical for our longitudinal studies."

Principal Investigator

Genomics Research Lab

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

8 Citations

Fluorogenic oligonucleotide cleavage probes with a branched linker improve RNA detection

Maru B, Edinboro A, Katolik A, et al. Nucleic Acids Research. 2025.

Novel glycerol-based 'X' linker design for TaqMan-type probes achieving near single-copy detection and four-color multiplex RT-qPCR capability.

View DOI

15 Citations

Generation of densely labeled oligonucleotides for the detection of small genomic elements

Cell Reports Methods. 2024.

Nanoscopy-compatible oligonucleotide probes (NOVA probes) with multiple fluorophores for detection of genomic loci below kilobase level.

View DOI

12 Citations

Comparison of Quenching Efficiencies in Long Triple-Labeled and Double-Labeled TaqMan Probes

Demuth J, Kantor M, Kucera R, et al. Bioconjugate Chemistry (ACS). 2022.

Systematic comparison of quenching efficiencies demonstrating that shortening fluorophore-quencher distance is key for FRET efficiency.

View DOI

18 Citations

Optimization and performance evaluation of double-stranded probe in real-time PCR

Han YJ, Liu LY, Liu QQ, Wang SQ. Analytical Biochemistry. 2022.

Optimization of double-stranded probe design for improved detection sensitivity in HBV and SARS-CoV-2 diagnostics.

View DOI

25 Citations

DNA-Aptamer-Based qPCR Using Light-Up Dyes for the Detection of Nucleic Acids

ACS Omega (ACS). 2023.

Novel light-up dye chemistry for qPCR monitoring achieving 800 copies/μL detection limit with linear standard curves.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our diagnostic probe synthesis service.

We offer a comprehensive range of fluorophores including FAM, HEX, TET, JOE, Cy3, Cy5, Cy5.5, ROX, and TAMRA. Quenchers include BHQ1, BHQ2, DABCYL, Iowa Black FQ/RQ, and our proprietary ZEN and TAO dual-quenchers. We also offer MGB and LNA modifications for specialized applications.

Every probe undergoes ESI mass spectrometry verification to confirm exact sequence and purity. Capillary electrophoresis analysis is performed for all purified products. For diagnostic grade probes, we provide complete Certificate of Analysis with batch records including purity, molecular weight verification, and quantity confirmation.

Standard qPCR probes ship within 3-5 business days for most orders. FISH probes and modified probes typically require 5-7 business days. Diagnostic grade probes with extended QC documentation require 7-10 business days. Rush services are available for urgent projects.

Yes, we offer ISO 13485 certified manufacturing for diagnostic grade probes. Our diagnostic grade products include complete batch records, full traceability documentation, and Certificate of Analysis suitable for IVD development and regulatory submissions. Contact us for specific regulatory compliance requirements.

We offer multiple purification options: standard desalting for routine applications, cartridge purification for cloning and sequencing, HPLC purification for analytical applications, and PAGE purification for high purity requirements. For diagnostic probes, we recommend HPLC or dual purification to ensure maximum purity.

Yes, we specialize in multiplex probe synthesis with validated fluorophore combinations supporting up to 6 targets in a single reaction. Our technical team can assist with probe design optimization for multiplex assays to ensure minimal spectral overlap and optimal performance.

Diagnostic Probes Oligos Synthesis

Why Choose Us

qPCR Probes

FISH Probes

Molecular Diagnostics

Precision Diagnostic Probe Synthesis

Proprietary Synthesis Platform

Comprehensive Quality Control

qPCR Probes

FISH Probes

Multiplex Solutions

Ready to Start Your Diagnostic Project?

Advanced Probe Synthesis Technologies

Phosphoramidite Chemistry

Advanced QC Systems

Dual-Quencher Technology

Fluorophore & Quencher Options

Specialized Modifications

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Sequence Design

Synthesis

QC Verification

Documentation

Delivery

Diverse Applications Across Diagnostics

Real-Time PCR Detection

Infectious Disease Detection

SNP Genotyping

What Our Clients Say

Scientific Foundation

Fluorogenic oligonucleotide cleavage probes with a branched linker improve RNA detection

Generation of densely labeled oligonucleotides for the detection of small genomic elements

Comparison of Quenching Efficiencies in Long Triple-Labeled and Double-Labeled TaqMan Probes

Optimization and performance evaluation of double-stranded probe in real-time PCR

DNA-Aptamer-Based qPCR Using Light-Up Dyes for the Detection of Nucleic Acids

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources

Request a Quote

Diagnostic Probes Oligos Synthesis

Why Choose Us

qPCR Probes

FISH Probes

Molecular Diagnostics

Precision Diagnostic Probe Synthesis

Proprietary Synthesis Platform

Comprehensive Quality Control

qPCR Probes

FISH Probes

Multiplex Solutions

Ready to Start Your Diagnostic Project?

Advanced Probe Synthesis Technologies

Phosphoramidite Chemistry

Advanced QC Systems

Dual-Quencher Technology

Fluorophore & Quencher Options

Specialized Modifications

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Sequence Design

Synthesis

QC Verification

Documentation

Delivery

Diverse Applications Across Diagnostics

Real-Time PCR Detection

Infectious Disease Detection

SNP Genotyping

What Our Clients Say

Scientific Foundation

Fluorogenic oligonucleotide cleavage probes with a branched linker improve RNA detection

Generation of densely labeled oligonucleotides for the detection of small genomic elements

Comparison of Quenching Efficiencies in Long Triple-Labeled and Double-Labeled TaqMan Probes

Optimization and performance evaluation of double-stranded probe in real-time PCR

DNA-Aptamer-Based qPCR Using Light-Up Dyes for the Detection of Nucleic Acids

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources