Mammalian Protein Purification Service

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We provide specialized Mammalian Protein Purification Services to isolate high-quality proteins from mammalian cells for a wide range of applications in pharmaceutical research, biotechnology, and therapeutic development. Our advanced techniques and state-of-the-art equipment ensure the isolation of proteins with high purity, yield, and functionality, tailored to meet the specific needs of your research and development projects.

Selection of high-performance liquid chromatography (HPL() methods for biomolecule separation according to bioproduct properties. MW: molecular weight(PS Corrêa, et al.,2020)

Overview Service Process Examples and Solutions Frequently Asked Questions

Overview

Mammalian protein purification involves isolating proteins from mammalian cells while preserving their native structure, post-translational modifications, and functionality. Compared to the purification of proteins from simpler organisms like bacteria or yeast, mammalian protein purification presents unique challenges and requires specialized techniques to maintain the complex characteristics and modifications of mammalian proteins.

Differences Between Mammalian Protein Purification and Ordinary Protein Purification

Post-Translational Modifications (PTMs): Mammalian proteins often undergo complex PTMs such as glycosylation, phosphorylation, and acetylation, which are essential for their function. These modifications are typically absent or simpler in proteins expressed in bacterial or yeast systems.
Protein Complexity: Mammalian proteins can be part of large, multi-subunit complexes that are difficult to purify intact. Maintaining these complexes requires careful handling and specific conditions.
Expression Levels: Proteins expressed in mammalian cells are often present at lower levels compared to those produced in bacterial systems, necessitating more sensitive and efficient purification techniques.
Cellular Environment: The cellular environment in mammalian systems is more complex, requiring additional steps to remove contaminants such as host cell proteins, nucleic acids, and lipids.

Methods for Mammalian Protein Purification

Method	Description	Applications
Affinity Chromatography	Uses specific interactions between a protein and a ligand attached to a chromatography matrix to purify the target protein with high specificity.	Commonly used for purifying tagged proteins (e.g., His-tag, FLAG-tag) and proteins with specific binding partners (e.g., antibodies).
Immunoprecipitation (IP)	Utilizes antibodies to specifically bind and isolate target proteins from complex mixtures.	Ideal for studying protein-protein interactions and purifying low-abundance proteins from mammalian cells.
Size Exclusion Chromatography (SEC)	Separates proteins based on their size and shape, allowing the isolation of protein complexes in their native state.	Suitable for final polishing steps to obtain pure and homogeneous protein samples, and for studying protein complexes.
Ion Exchange Chromatography (IEX)	Separates proteins based on their charge, helping to remove contaminants with different charge properties.	Useful for intermediate purification steps to improve protein purity and separate isoforms.
Hydrophobic Interaction Chromatography (HIC)	Exploits the hydrophobic properties of proteins to facilitate separation and purification.	Suitable for purifying proteins with exposed hydrophobic regions, often used after initial affinity or ion exchange chromatography.
Reverse Phase Chromatography (RPC)	Utilizes hydrophobic interactions between proteins and the chromatography matrix, providing an alternative method for purifying hydrophobic proteins.	Suitable for purifying highly hydrophobic proteins and peptides.
Ultracentrifugation	Uses high-speed centrifugation to separate proteins based on their density, often used to isolate subcellular fractions and large protein complexes.	Suitable for initial isolation of cellular fractions and purifying large protein complexes.
Lectin Affinity Chromatography	Specifically binds glycoproteins based on their carbohydrate moieties, allowing for selective purification.	Ideal for purifying glycosylated proteins and studying glycoproteins.

Service Process

Project Consultation: Collaborating with clients to understand their specific protein purification goals, including target proteins, desired purity, and application requirements.
Sample Preparation: Preparing the protein samples using optimized protocols to ensure effective purification.
Purification Strategy Design: Designing a customized purification strategy based on the protein's properties and project needs.
Protein Purification: Implementing the chosen purification methods using advanced equipment and technologies to achieve high purity and yield.
Quality Assurance: Conducting rigorous quality control assays to validate the purity, yield, and functionality of the purified protein.
Delivery and Support: Delivering the purified protein with comprehensive documentation and providing ongoing technical support.

For more information on our Mammalian Protein Purification Service, please fill out our online inquiry form. One of our specialists will get back to you promptly to discuss your specific needs.

Examples and Solutions

Case Examples	Solutions We Offer
Therapeutic Protein Production: Purifying monoclonal antibodies for therapeutic use.	Utilizing affinity chromatography for high-purity antibody purification, ensuring functionality and safety for clinical applications.
Biochemical Research: Isolating enzymes for biochemical assays.	Employing ion exchange and size exclusion chromatography to achieve high purity and activity for precise biochemical studies.
Structural Biology: Purifying proteins for crystallization and structural analysis.	Using a combination of purification methods to obtain highly pure proteins suitable for crystallography and NMR studies.
Industrial Enzyme Production: Purifying enzymes for industrial processes.	Tailoring purification strategies to maximize yield and stability, ensuring enzymes meet industrial standards.
Proteomics: Isolating specific proteins from complex mixtures for proteomic studies.	Implementing high-resolution purification techniques to ensure accurate protein identification and quantification.
Vaccine Development: Purifying antigens for vaccine production.	Designing purification protocols to achieve high-purity antigens, critical for effective vaccine formulation.

Frequently Asked Questions

Q: What mammalian protein purification methods do you offer?

A: We offer a wide range of mammalian protein purification methods, including affinity chromatography, ion exchange chromatography, size exclusion chromatography, hydrophobic interaction chromatography, and reverse phase chromatography.

Q: How do you ensure the purity of the mammalian proteins?

A: We employ rigorous quality control assays, including SDS-PAGE, Western blotting, and mass spectrometry, to ensure high purity and functionality of the purified proteins.

Q: Can you customize the purification process for specific mammalian proteins?

A: Yes, we provide tailored purification strategies to meet the unique needs of each protein and project, ensuring optimal results.

Q: How do you handle sample preparation?

A: We use optimized protocols for sample preparation to ensure that the mammalian proteins are effectively extracted and solubilized for purification.

Q: What documentation do you provide with the purified proteins?

A: We provide detailed reports on the purification process, including production conditions, purification methods, and quality control results.

Q: What kind of support do you offer post-delivery?

A: We offer ongoing technical support and troubleshooting assistance to ensure the successful utilization of the purified proteins in your applications.

Please note that all services are for research use only. Not intended for any clinical use.

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