CD Biosynsis delivers high-purity, custom RNA Synthesis Services essential for gene regulation studies, therapeutics, and functional genomics. We specialize in synthesizing a wide array of RNA molecules, including: high-quality RNA Oligonucleotides , functional small RNAs such as siRNA and miRNA tools, and the crucial sgRNA components for CRISPR-Cas9 systems. Our platform is robustly supported by extensive RNA Modification expertise and strict HPLC/PAGE purification, ensuring high yield, exceptional purity, and guaranteed sequence fidelity for even the most challenging RNA sequences.
Get a QuoteThe successful outcome of RNA applications, particularly in gene knockdown ( siRNA ) and CRISPR gene editing ( sgRNA ), relies heavily on the quality and purity of the synthesized molecule. Impurities or truncated sequences can lead to off-target effects, toxicity, or inefficient delivery. Our synthesis protocols prioritize high-yield solid-phase synthesis followed by stringent HPLC or PAGE purification to remove contaminating species. Furthermore, our ability to introduce Custom RNA Modifications dramatically enhances the stability and in vivo efficacy of therapeutic RNA constructs.
Synthesis of highly efficient, duplex short interfering RNA ( siRNA ) for potent RNA interference ( RNAi ) and gene knockdown applications.
Synthesis of microRNA ( miRNA ) precursors or mature sequences for studies in post-transcriptional gene regulation.
Specialized miRNA mimics ( Agomirs ) and inhibitors ( Antagomirs ) for robust activation or suppression of specific miRNA pathways in vivo .
High-quality, chemically - synthesized single guide RNA ( sgRNA ) for CRISPR gene editing applications, ensuring maximum cleavage efficiency.
Bioinformatic design service to select high-specificity, high-activity sgRNA targets, minimizing off-target effects.
Construction of targeted or genome-wide sgRNA libraries for high-throughput functional screens, including Human Genome Knockout Libraries and Livestock/Poultry CRISPR Knockout Libraries .
Incorporation of diverse modifications (e.g., 2'- O - Methyl , Phosphorothioate , fluorescent dyes ) to improve stability, tracking, or function.
Synthesis of general purpose RNA oligos (primers, linkers) for basic research needs, up to 80 bases.
Synthesis of mRNA or lncRNA transcripts exceeding 80 bases, typically utilizing in vitro transcription ( IVT ) methods.
Our process emphasizes high purity and fidelity, critical for all downstream RNA applications.
Design & Sequence Validation
Chemical/Enzymatic Synthesis
Purification & QC Verification
Delivery & Storage Recommendations
Sequence confirmation and target validation (especially for siRNA/sgRNA ).
Optional sgRNA Design Service to optimize CRISPR efficiency.
Consultation on the appropriate modifications ( RNA Modifications ) based on application ( in vitro vs. in vivo ).
Oligo Synthesis: Solid - phase chemical synthesis for short, modified, or sgRNA constructs.
Long RNA Synthesis: IVT (in vitro transcription) for longer mRNA or lncRNA transcripts.
Products delivered lyophilized or in solution, clearly labeled with purity and QC data.
Detailed certificate of analysis ( CoA ) with MS and purity data included.
Guaranteed High Purity ( >95% )
All functional RNAs ( siRNA , miRNA , sgRNA ) are purified by HPLC or PAGE to minimize off-target effects and toxicity.
Advanced RNA Modifications
Expertise in complex RNA Modifications to boost in vivo stability and pharmacokinetics for therapeutic applications.
sgRNA Library Expertise
Fast and accurate construction of sgRNA Libraries for genome-wide and targeted CRISPR screening in various species.
Comprehensive RNAi Tools
Full suite of RNAi reagents, including siRNA , miRNA , Agomirs, and Antagomirs , tailored for functional knockdown and activation studies.
"The CRISPR-Cas9 sgRNA Synthesis purity was excellent. Our gene editing efficiency was significantly higher than with our previous vendor, which sped up our cell line development by weeks."
Dr. Alan Rivas, Lab Director, Functional Genomics
"We ordered miRNA Agomir with specific 2'- O - Methyl modifications. CD Biosynsis verified the modification using MS and the Agomir showed robust, stable expression in vivo ."
Ms. Sarah Jenkins, VP of Therapeutic Development
"Their Custom sgRNA Libraries for the Human Genome Knockout screen provided the high coverage and low bias needed to successfully identify novel therapeutic targets."
Dr. Lena Koo, PI , Target Discovery Institute
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has být pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the typical purity level for your functional RNAs ( siRNA, sgRNA )?
For functional RNAs like siRNA and sgRNA ( CRISPR-Cas9 sgRNA Synthesis ), we guarantee a minimum purity of >95% , achieved via HPLC purification, which is essential to eliminate impurities that cause off-target effects.
What is the advantage of using Custom RNA Modifications ?
Modifications, such as 2'- O - Methyl or Phosphorothioate bonds , significantly increase the nuclease resistance and serum half-life of RNA (e.g., siRNA or miRNA Agomir ), making them suitable for in vivo therapeutic studies.
Can you synthesize RNA transcripts longer than 100 bases?
Yes, our Long RNA Synthesis Service utilizes optimized in vitro transcription ( IVT ) to produce high-yield mRNA or long non-coding RNA ( lncRNA ) transcripts in milligram to gram quantities.
How do you ensure the quality of sgRNA libraries?
Our Custom sgRNA Libraries (including Human Genome Knockout Libraries ) are synthesized with high uniformity and minimal bias, and the final quality is confirmed via Next - Generation Sequencing ( NGS ) analysis.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS , FDA ).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.