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Trusted by Leading Research & Pharma Institutions

Custom RNA Synthesis Services

Accelerate your research with custom RNA oligos consistently manufactured to your exact specifications. From routine siRNA to complex therapeutic oligonucleotides, we deliver high-quality synthetic RNA with advanced modification options for enhanced stability and efficacy.

NGS Verified
200+ Modifications
Up to 160 nt
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Trusted by leading research and pharmaceutical institutions

MIT
Pfizer
Stanford
Roche
Johns Hopkins
Novartis

Why Choose Us

Proprietary phosphoramidite chemistry
200+ modification options
HPLC/MALDI-TOF QC on every oligo
Expert consultation included

Custom RNA Oligos

siRNA, miRNA, sgRNA up to 160 nt

Therapeutic Quality

ISO 13485 GMP-grade available

Flexible Modifications

2'-OMe, 2'-F, LNA, PS backbone

Market CAGR
11.2%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive Custom RNA Synthesis Solutions

Our platform combines established phosphoramidite chemistry with advanced modification capabilities to deliver high-quality RNA oligos for research and therapeutic applications.

Phosphoramidite Chemistry

Proven solid-phase synthesis using phosphoramidite chemistry with optimized coupling efficiency. Our proprietary synthesis platform delivers more full-length RNA product with rigorous QC at every step.

  • Standard RNA: 5-80 nt
  • Long RNA: up to 160 nt
  • High coupling efficiency

Therapeutic-Grade Quality

Every RNA oligo undergoes rigorous quality control including MALDI-TOF mass spectrometry and HPLC analysis. GMP-grade manufacturing available for therapeutic applications.

  • MALDI-TOF MS verification
  • HPLC purity analysis
  • ISO 13485 GMP available

200+ Modifications

Extensive modification options including 2'-OMe, 2'-F, LNA, phosphorothioate, and fluorescent dyes.

Complete QC Reports

Every order includes detailed mass spectrometry and purity analysis reports.

Expert Support

Free consultation for sequence design and modification selection.

Ready to Start Your RNA Project?

Get a customized quote for your custom RNA synthesis needs.

Technology Platform

Advanced RNA Synthesis Technologies

Industry-leading synthesis platforms ensuring high-quality output for every project.

Phosphoramidite Chemistry

Optimized phosphoramidite chemistry for RNA synthesis, delivering high-quality full-length products with superior purity.

High Efficiency Solid-Phase

Advanced QC Systems

Every RNA oligo undergoes MALDI-TOF mass spectrometry and HPLC analysis to ensure sequence accuracy and purity.

MALDI-TOF HPLC

Flexible Modifications

200+ modification options including sugar modifications, backbone changes, and fluorescent labels for diverse applications.

2'-OMe LNA PS

Sugar Modifications

2'-OMe 2'-O-Methyl RNA
2'-F 2'-Fluoro RNA
LNA Locked Nucleic Acid
2'-MOE 2'-O-Methoxyethyl

Backbone & Terminal Modifications

PS Phosphorothioate linkage
5' / 3' Terminal phosphate, amine, thiol
Dye FAM, HEX, Cy3, Cy5, Alexa Fluor
Quencher BHQ, TAMRA, DABCYL
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter Standard RNA Long RNA siRNA
Length Range 5 - 80 nt 81 - 160 nt 19 - 25 bp duplex
Scale Range 0.025 - 10 μmol 0.25 - 5 μmol 50 - 200 nmol
Purification Desalt, Cartridge, HPLC HPLC, PAGE HPLC purified
Quality Control MALDI-TOF MS MALDI-TOF MS MALDI-TOF, PAGE
Modifications 200+ options Custom Standard + custom
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Consultation

Submit sequence and modification requirements

2

Design

Sequence verification and optimization

3

Synthesis

Phosphoramidite-based synthesis

4

QC

MALDI-TOF MS and HPLC analysis

5

Delivery

Lyophilized with comprehensive report

Applications

Diverse Applications Across Biotechnology

Our RNA synthesis services support research and development in multiple fields.

RNAi & Gene Silencing

High-quality siRNA and miRNA for effective gene knockdown experiments. Our modified oligos ensure enhanced stability and sustained silencing effects in diverse cell types.

  • siRNA duplexes for gene silencing
  • miRNA mimics and inhibitors
  • 2'-F and 2'-OMe modifications for stability
  • Phosphorothioate linkages for nuclease resistance
>90%
Knockdown efficiency

Therapeutic Oligonucleotides

ASOs, aptamers, and anti-sense oligonucleotides with chemical modifications for enhanced therapeutic potential. GMP-grade manufacturing available for clinical applications.

  • Antisense oligonucleotides (ASO)
  • Nucleic acid aptamers
  • Stereopure oligonucleotides
  • ISO 13485 GMP manufacturing
200+
Modification options

Molecular Diagnostics

Fluorescently labeled probes and primers for qPCR, NGS, and molecular detection assays. High purity ensures reliable results in diagnostic applications.

  • qPCR probes and primers
  • NGS adapter sequences
  • Molecular beacon probes
  • In vitro diagnostic (IVD) reagents
99.5%
Purity guarantee
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The RNA oligo quality is consistently excellent. MALDI-TOF verification gives us confidence in every experiment. Highly recommend for any RNAi project."

S
Senior Scientist
Biotechnology Company

"Fast turnaround and excellent technical support. The modification options available have been crucial for our therapeutic oligonucleotide development."

R
Research Director
Pharmaceutical Company

"We've been using this service for our CRISPR and diagnostic projects. Consistent quality and reliable results every time. The GMP option is excellent."

P
Principal Investigator
Academic Research Institution
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

316 Citations

Recent progress in non-native nucleic acid modifications

McKenzie LK, El-Khoury R, Thorpe JD, et al. Chemical Society Reviews. 2021.

Comprehensive review of modified nucleic acids for therapeutic oligonucleotides including ASOs and siRNAs.

View DOI
106 Citations

Enzymatic DNA synthesis by engineering terminal deoxynucleotidyl transferase

Lu X, Li J, Li C, Lou Q, Peng K, Cai B, Liu Y, Yao Y, et al. ACS Catalysis. 2022.

Engineering TdT for enzymatic DNA/RNA synthesis with reversible terminators enabling high-throughput production.

View DOI
96 Citations

Catalytic asymmetric and stereodivergent oligonucleotide synthesis

Featherston AL, Kwon Y, Pompeo MM, Engl OD, et al. Science. 2021.

Breakthrough in catalytic asymmetric synthesis of all possible oligonucleotide stereoisomers.

View DOI
57 Citations

Automated high-throughput DNA synthesis and assembly

Ma Y, Zhang Z, Jia B, Yuan Y. Heliyon (Cell Press). 2024.

Comprehensive review of automated synthesis platforms and biofoundries for accurate high-throughput nucleic acid production.

View DOI
85 Citations

An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts

Dousis A, Ravichandran K, Hobert EM, Moore MJ, Rabideau AE. Nature Biotechnology. 2022.

Engineered T7 RNAP variant for producing high-quality mRNA with reduced dsRNA impurities.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

RNA synthesis requires additional protection of the 2'-hydroxyl group during the phosphoramidite process. Common protecting groups include TBDMS, TOM, or ACE. This makes RNA synthesis more complex than DNA synthesis, but our optimized protocols ensure high coupling efficiency and purity for all RNA products.
For optimal siRNA stability and efficacy, we recommend: 2'-F modifications on the sense strand for nuclease resistance, 2'-OMe modifications on both strands, and phosphorothioate linkages at the 3' ends. These modifications enhance serum stability while maintaining silencing efficiency.
All RNA oligos are shipped lyophilized (freeze-dried) to ensure maximum stability. Upon arrival, store at -20°C or -80°C. Dissolved oligos should be stored at -20°C and are stable for at least 6 months when properly handled. We recommend using RNase-free water and tubes for resuspension.
We offer multiple purification options: standard desalting for routine applications, cartridge purification for cloning and sequencing, HPLC for high purity requirements, and PAGE for long oligos or complex modifications. For therapeutic applications, we recommend HPLC or PAGE purification.
Yes, we offer ISO 13485 certified GMP-grade RNA synthesis for therapeutic applications. Our GMP service includes comprehensive documentation, regulatory support, and scalability from research to clinical trial material. Contact us for a customized quote based on your project requirements.
Every RNA oligo undergoes rigorous quality control including MALDI-TOF mass spectrometry to verify sequence identity and HPLC analysis for purity assessment. For duplexed products like siRNA, we also perform PAGE analysis to confirm proper annealing. Detailed QC reports are included with every order.

Ready to Start Your Project?

Get a customized quote for your Custom RNA Synthesis project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Custom RNA oligos synthesis
· Custom siRNA Synthesis
· Custom miRNA synthesis
· Long RNA synthesis service
· Custom RNA modifications
· Human Genome Knockout Libraries Construction Service

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
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