Synthetic Biology
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Trusted by Leading Research & Pharma Institutions

Custom DNA Synthesis Services

Accelerate your research with custom DNA oligos consistently manufactured to your exact specifications using our proprietary synthesis platform. From routine PCR primers to complex gene constructs, we deliver high-quality synthetic DNA with industry-leading turnaround times.

ESI-MS Verified
99.4% Coupling
24hr Turnaround
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Trusted by leading research and pharmaceutical institutions

MIT
Pfizer
Stanford
Roche
Johns Hopkins
Merck

Why Choose Us

Better coupling efficiency than competitors
1,000+ modification options
90% orders ship within 24 hours
ESI-MS on every oligo

Custom Oligos

10-200 bases, standard to ultramer

Gene Fragments

300 bp - 5,000 bp, linear dsDNA

Clonal Genes

NGS-verified, cloned into vector

Coupling Efficiency
99.4%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive Custom DNA Synthesis Solutions

Our proprietary synthesis platforms deliver more full-length product with better coupling efficiency than other suppliers.

Proprietary Synthesis Platform

We formulate all key synthesis reagents in-house and design, manufacture, and calibrate our own DNA synthesizers. This vertical integration enables us to internally monitor equipment performance and improve each step of the oligonucleotide synthesis process.

  • 99.4% coupling efficiency (vs 98.5% competitors)
  • In-house formulated reagents
  • Custom engineered synthesizers

Industry-Leading Quality Control

Every oligo we synthesize undergoes ESI mass spectrometry analysis to confirm that the full-length oligos in your tube possess the exact sequence you ordered. Capillary electrophoresis traces are provided for purified oligos.

  • ESI-MS verification on every oligo
  • CE purity analysis
  • NGS verification for genes >1kb

Fast Turnaround

>90% of small-scale DNA orders ship within 24 hours.

1,000+ Modifications

Fluorophores, quenchers, modified bases, linkers, and spacers.

Flexible Formats

Tubes, plates, pools, duplexes, custom normalization.

Ready to Start Your Project?

Get a customized quote for your custom DNA synthesis needs.

Technology Platform

Advanced Synthesis Technologies

Proprietary platforms designed for optimal performance and reliability.

Phosphoramidite Chemistry

Optimized phosphoramidite chemistry with 99.4% coupling efficiency, delivering more full-length product than competitors at 98.5%.

99.4% Efficiency Proprietary

Quality Verification

Every oligo undergoes ESI mass spectrometry analysis. Capillary electrophoresis traces provided for purified products.

ESI-MS CE Analysis

Flexible Purification

Multiple purification options to meet your specific application requirements, from standard desalting to HPLC and PAGE.

Desalt HPLC PAGE

Quality Control

MS ESI mass spectrometry on every oligo
CE Capillary electrophoresis purity
NGS Next-gen sequencing for genes >1kb
COA Certificate of Analysis included

Purification Options

STD Standard desalting for PCR
CART Cartridge for cloning
HPLC High purity ≥85% for therapeutics
PAGE Ultra-high purity ≥95%
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter Custom DNA Oligos Gene Fragments Clonal Genes
Length Range 10 - 200 nt 300 bp - 5,000 bp 300 bp - 5,000 bp
Coupling Efficiency 99.4% N/A N/A
Turnaround Time 4-7 business days 6-10 business days 9-12 business days
Scale Range 0.025 - 10 μmol 100-1000 ng 1 μg - 1 mg
Purification Desalt, Cartridge, HPLC, PAGE Standard Microprep to Maxiprep
Verification ESI-MS Size/purity check NGS-verified
Workflow

Streamlined Process from Design to Delivery

Our proven workflow ensures quality and efficiency at every stage.

1

Order

Submit sequence and specifications

2

Synthesis

Proprietary platform synthesis

3

QC

ESI-MS and CE verification

4

Packaging

Lyophilized in tubes or plates

5

Delivery

Fast shipping with QC report

Applications

Diverse Applications Across Biotechnology

Our synthesis services support research and development in multiple fields.

NGS & PCR Applications

High-quality oligos for next-generation sequencing, qPCR, and multiplex PCR applications. Our proprietary synthesis platform delivers consistent results for demanding applications.

  • NGS adapters and primers
  • qPCR probes and primers
  • Multiplex PCR primer pools
  • Target capture probes
99.4%
Coupling efficiency

CRISPR Genome Editing

Custom gRNAs, donor templates, and HDR repair oligos for precise genome engineering applications. Quality matters when you're editing genomes.

  • Single guide RNA (sgRNA) synthesis
  • CRISPR library construction
  • Donor template design
  • HDR repair oligos
350 nt
Max pool length

Synthetic Biology

Gene fragments and clonal genes for synthetic biology applications. From gene construction to pathway engineering, we support your innovative research.

  • Gene construction
  • Pathway engineering
  • Enzyme optimization
  • Data storage applications
5 kb
Max gene length
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The coupling efficiency is noticeably better than our previous supplier. We're getting more full-length product and better results in our PCR applications. The ESI-MS verification gives us confidence in every order."

S
Senior Scientist
Molecular Diagnostics Company

"Fast turnaround times have been critical for our research deadlines. The 24-hour shipping for standard oligos is a game-changer. Quality control documentation is comprehensive and easy to access."

R
Research Director
Academic Research Institution

"We've been using this service for our CRISPR library construction. The oPools quality is excellent with high uniformity. Customer support is responsive and helpful with complex orders."

P
Principal Investigator
Genome Engineering Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

60 Citations

On-demand synthesis of phosphoramidites

Sandahl AF, Nguyen TJD, Hansen RA, et al. Nature Communications. 2021.

Chemical oligonucleotide synthesis relies upon phosphoramidites. This work introduces on-demand synthesis methods for DNA synthesis phosphoramidite chemistry.

View DOI
57 Citations

Automated high-throughput DNA synthesis and assembly

Ma Y, Zhang Z, Jia B, Yuan Y. Heliyon (Cell Press). 2024.

Comprehensive review of automated synthesis platforms, DNA assembly techniques, and biofoundries for accurate high-throughput DNA synthesis.

View DOI
10 Citations

Investigating the activation kinetics of phosphoramidites for oligonucleotide synthesis

Laraman FJ, Fisk H, Whittaker DTE, et al. Organic Process Research & Development (ACS). 2021.

Study of phosphoramidite activation kinetics for oligonucleotide synthesis using custom-made synthesis columns.

View DOI
26 Citations

Synthesis of substituted Cy5 phosphoramidite derivatives and their incorporation into oligonucleotides using automated DNA synthesis

Meares A, Susumu K, Mathur D, Lee SH, Mass OA, et al. ACS Omega. 2022.

Synthesis of substituted Cy5 phosphoramidite derivatives and incorporation into oligonucleotides using automated DNA synthesis.

View DOI
15 Citations

A unified phosphoramidite platform for the synthesis of morpholino oligonucleotides and diverse chimeric backbones

Ghosh A, Banerjee A, Gupta S, et al. Journal of the American Chemical Society (ACS). 2024.

Unified phosphoramidite platform for synthesis of morpholino oligonucleotides and diverse chimeric backbones following standard DNA synthesis protocols.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

We offer multiple purification options to meet your specific application requirements. Standard desalting is suitable for routine PCR applications. Cartridge purification is recommended for cloning and sequencing. For therapeutic or diagnostic applications requiring high purity, we recommend HPLC (≥85% purity) or PAGE (≥95% purity).
Every oligo we synthesize undergoes ESI mass spectrometry analysis to confirm that the full-length oligos in your tube possess the exact sequence you ordered. Capillary electrophoresis traces are provided for purified oligos. Gene constructs longer than 1 kb receive NGS verification.
>90% of small-scale, standard, desalted DNA oligo orders ship within 48 hours. Gene fragments with adapters: 6-10 business days. Gene fragments without adapters: 8-12 business days. Clonal genes: 9-12 business days for standard service. Express services are available for urgent projects.
Products are typically delivered as lyophilized (dried-down) in tubes or 96/384-well plates. For small orders (less than 10 genes), delivery in tubes is available at no additional cost. Clonal genes are delivered cloned into one of our catalog vectors or a customer-provided custom vector.
The maximum size for a gene fragment (linear double-stranded DNA) is 5,000 bp (5 kb). The maximum size for a clonal gene (a gene cloned into a vector) is also 5,000 bp. The minimum size for our gene synthesis products is 300 bp.
We offer more than 1,000 modification options including fluorophores (FAM, HEX, Cy3, Cy5), dark quenchers (BHQ1, BHQ2), modified bases, linkers, spacers, and many others. Both catalog and off-catalog modifications are available for custom oligo synthesis.

Ready to Start Your Project?

Get a customized quote for your Custom DNA Synthesis project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Gene Synthesis Service
· Chip Based DNA Synthesis
· Long DNA Synthesis Service
· Modified DNA Synthesis Service
· Plasmid DNA Preparation Service
· cDNA Libraries Construction Service

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
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