Synthetic Biology
Home / Services / By Technology / DNA and RNA Synthesis Services / Custom DNA Synthesis
Gene Synthesis Service

Custom Gene Synthesis for Research-Grade DNA Constructs

From codon-optimized ORFs to complex multi-gene assemblies, our gene synthesis service delivers sequence-verified DNA constructs engineered for your exact experimental requirements.

We provide end-to-end custom gene synthesis with rigorous quality control at every step. Every construct is sequence-verified and delivered in the vector of your choice, ready for downstream applications including protein expression, CRISPR genome engineering, and synthetic biology. Our platform combines automated synthesis with expert sequence optimization to handle even the most challenging GC-rich or repeat-heavy sequences.

  • 100% sequence accuracy verification
  • Codon optimization for any expression host
  • Custom vector and cloning options
  • QC documentation with every delivery
Request a consultation

Project-Ready DNA, Delivered

Every construct is sequence-verified and shipped with full QC documentation.

  • Sequence-verified clones
  • Choice of delivery vector
  • Codon optimization available
  • QC documentation included
Sequence-Verified
Codon-Optimized
Custom Cloning
Solutions

Gene Synthesis Tailored to Your Research

Whether you need a simple ORF or a complex multi-gene construct, our gene synthesis platform is designed to deliver accurate, high-quality DNA for a wide range of applications.

Standard Gene Synthesis

Our core service for synthesizing genes from 300 bp to over 5 kb with high accuracy. Ideal for cloning, protein expression, and functional studies.

  • Sequence verification via Sanger or NGS
  • Cloning into standard vectors
  • Optional codon optimization

Complex & Difficult Sequences

Specialized protocols for GC-rich, repeat-heavy, or highly structured sequences that often fail with standard synthesis approaches.

  • Proprietary synthesis algorithms
  • Handling of hairpins and repeats
  • Alternative assembly strategies

Codon Optimization

Optimize your gene for expression in E. coli, yeast, insect, or mammalian cells to maximize protein yield.

  • Host-specific codon usage tables
  • mRNA structure minimization
  • Restriction site avoidance

Custom Cloning

Clone your synthesized gene into any vector, including expression, lentiviral, or CRISPR vectors.

  • Compatible with common restriction sites
  • Gateway or Gibson Assembly options
  • Sequence-verified final construct

Gene Fragments

Order synthetic DNA fragments for CRISPR donor templates, sgRNA expression cassettes, or as building blocks for larger assemblies.

  • Lengths from 100 bp
  • Purified or unpurified options
  • Rapid turnaround for small fragments
Technology

Precision Synthesis and Verification

Our platform integrates advanced synthesis chemistry with rigorous QC to ensure every construct meets your specifications.

Automated Synthesis Platform

Fully automated gene synthesis covering the entire process from sequence design to final clone, ensuring consistency and scalability.

Key Capabilities
  • High-throughput parallel synthesis
  • Error-correction technology
  • Scalable from single genes to libraries
Quality Control
  • Sequence verification of final clones
  • QC data provided with each order
  • Traceable documentation

Sequence Optimization Algorithms

Advanced algorithms that optimize codon usage, remove repeat regions, and minimize secondary structures to improve synthesis success and expression.

Optimization Parameters
  • Codon adaptation index (CAI)
  • GC content balancing
  • Repeat sequence avoidance
Expression Hosts
  • E. coli, yeast, insect, mammalian
  • Cell-free expression systems
  • Custom host profiles

Verification & QC

Every construct is verified by sequencing to ensure 100% sequence accuracy before shipment.

Verification Methods
  • Sanger sequencing for smaller constructs
  • NGS for larger or complex genes
  • Restriction digest analysis
Deliverables
  • Purified plasmid DNA
  • Sequence files (GenBank, FASTA)
  • QC report

Standard Features

  • Sequence verification
  • Choice of vector
  • QC documentation

Optional Add-ons

  • Codon optimization
  • Custom cloning
  • Mutagenesis
Comparison

Choose the Right Synthesis Option

Compare our standard and advanced gene synthesis services to find the best fit for your project.
Sequence Length Up to 3 kb Up to 5 kb >5 kb (custom)
Sequence Verification Sanger sequencing NGS verification NGS + Sanger
Codon Optimization Optional Included Included
Complex Sequences Standard protocols Enhanced protocols Specialized handling
Cloning Options Standard vectors Standard + custom Any vector
QC Documentation Basic QC report Detailed QC report Comprehensive QC
Technical Support Subject to customer service consultation Subject to customer service consultation Subject to customer service consultation
Workflow

How Our Gene Synthesis Service Works

A streamlined, transparent process from sequence submission to delivered construct.

Submit Your Sequence

Provide your gene sequence in FASTA or GenBank format, or specify the protein sequence for codon optimization. Include any vector or cloning requirements.

Sequence Analysis & Optimization

Our team reviews your sequence for synthesis feasibility, identifies potential problem areas (repeats, GC-rich regions), and performs codon optimization if requested.

Synthesis & Cloning

Your gene is synthesized using our automated platform, with error-correction technology to ensure accuracy. The construct is then cloned into your chosen vector.

Quality Control & Delivery

Every clone is sequence-verified, and you receive purified plasmid DNA along with full QC documentation, including sequence files and a QC report.

Applications

Gene Synthesis for Diverse Research Areas

Our custom gene synthesis service supports a wide range of applications across life sciences.

Optimized Genes for High-Yield Expression

Obtain codon-optimized genes for efficient protein expression in your preferred host system. Our synthesis service ensures your gene is tailored for maximum yield, whether you're working with E. coli, yeast, insect cells, or mammalian cells.

  • Codon optimization for any host
  • Expression vector construction
  • Sequence-verified clones

Precise DNA Templates for Gene Editing

Generate donor templates for knock-in experiments, sgRNA expression cassettes, or homology-directed repair (HDR) constructs. Our synthesis service provides the precise DNA sequences needed for successful CRISPR-Cas9 genome editing.

  • HDR donor templates
  • sgRNA expression cassettes
  • Custom vector design

Build Complex Genetic Circuits

Assemble multi-gene constructs, metabolic pathways, and genetic circuits with our gene synthesis service. Our platform can handle complex assemblies, enabling you to design and build novel biological systems.

  • Multi-gene assembly
  • Pathway construction
  • Library synthesis
FAQ

Gene Synthesis Service: Common Questions

Find answers to frequently asked questions about our gene synthesis service.

  • Our standard gene synthesis service can produce constructs up to 5 kb in length. For larger genes or multi-gene assemblies, we offer custom solutions that can handle more complex constructs. Contact us to discuss your specific requirements.

  • Every construct is sequence-verified before shipment. We use Sanger sequencing for smaller constructs and NGS for larger or more complex genes. This ensures 100% sequence accuracy, and you receive the sequencing data with your order.

  • Yes, our platform is equipped to handle complex sequences, including GC-rich regions, repeats, and hairpin structures. We use specialized protocols and error-correction technology to successfully synthesize these challenging constructs.

  • Yes, we offer codon optimization for a variety of expression hosts, including E. coli, yeast, insect cells, and mammalian cells. Our algorithms adjust the codon usage to match the host's preferences, which can significantly improve protein expression levels.

  • We deliver your synthesized gene as purified plasmid DNA, typically in a standard cloning vector. We can also clone into your specified vector or provide linear DNA fragments. All deliveries include sequence files and a QC report.

Scientific Literature

References

  1. Turro E, Astle WJ, Megy K, et al. Whole-genome sequencing of patients with rare diseases in a national health system. Nature. 2020;583(7814):96-102. PubMed
  2. Roberts AM, Ware JS, Herman DS, et al. Integrated allelic, transcriptional, and phenomic dissection of the cardiac effects of titin truncations in health and disease. Sci Transl Med. 2015;7(270):270ra6. PubMed
  3. ICGC/TCGA Pan-Cancer Analysis of Whole Genomes Consortium. Pan-cancer analysis of whole genomes. Nature. 2020;578(7793):82-93. PubMed
  4. Okada Y, Wu D, Trynka G, et al. Genetics of rheumatoid arthritis contributes to biology and drug discovery. Nature. 2014;506(7488):376-381. PubMed
  5. Hautakangas H, Winsvold BS, Ruotsalainen SE, et al. Genome-wide analysis of 102,084 migraine cases identifies 123 risk loci and subtype-specific risk alleles. Nat Genet. 2022;54(2):152-160. PubMed
  6. Zebley CC, Zehn D, Gottschalk S. T cell dysfunction and therapeutic intervention in cancer. Nat Immunol. 2024;25(8):1344-1354. PubMed
  7. Coughlin CR 2nd, Swanson MA, Kronquist K, et al. The genetic basis of classic nonketotic hyperglycinemia due to mutations in GLDC and AMT. Genet Med. 2017;19(1):104-111. PubMed
  8. Langlois AWR, Chenoweth MJ, Twesigomwe D, et al. PharmVar GeneFocus: CYP2A6. Clin Pharmacol Ther. 2024;116(4):911-925. PubMed
  9. Sangkuhl K, Claudio-Campos K, Cavallari LH, et al. PharmVar GeneFocus: CYP2C9. Clin Pharmacol Ther. 2021;110(3):662-676. PubMed
  10. Botton MR, Whirl-Carrillo M, Del Tredici AL, et al. PharmVar GeneFocus: CYP2C19. Clin Pharmacol Ther. 2021;109(2):352-366. PubMed
  11. Walker LC, Hoya M, Wiggins GAR, et al. Using the ACMG/AMP framework to capture evidence related to predicted and observed impact on splicing: Recommendations from the ClinGen SVI Splicing Subgroup. Am J Hum Genet. 2023;110(7):1046-1067. PubMed
  12. Argüello RJ, Combes AJ, Char R, et al. SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution. Cell Metab. 2020;32(6):1063-1075.e7. PubMed

Ready to Start Your Project?

Get a customized quote for your Custom DNA Synthesis project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Gene Synthesis Service
· Chip Based DNA Synthesis
· Long DNA Synthesis Service
· Modified DNA Synthesis Service
· Plasmid DNA Preparation Service
· cDNA Libraries Construction Service

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
Get a free quote

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Quick Links

Home

Products

Services

About Us

Contact Us

Get In Touch

Address:

Tel:

Email:

Copyright © 2026 CD Biosynsis. All rights reserved.