Custom DNA Synthesis Service

Custom DNA Synthesis is the cornerstone of modern molecular and synthetic biology, providing researchers with the fundamental building blocks necessary for genetic engineering, gene expression, and pathway construction. From short primers and probes to complex full-length genes and large DNA fragments, access to high-quality, sequence-verified synthetic DNA is essential for accelerating the Design-Build-Test-Learn (DBTL) cycle.

CD Biosynsis offers end-to-end Custom DNA Synthesis services, backed by proprietary algorithms for codon optimization and advanced high-throughput synthesis platforms . We guarantee 100% sequence accuracy for cloned genes and offer industry-leading turnaround times, even for sequences with high GC content, complex secondary structures, or extensive repeats. Our services cover everything from standard oligo synthesis to the construction of complex, multi-kilobase gene fragments and cloned genes, allowing you to focus on the downstream application of your research.

Highlights

Choose our Custom DNA Synthesis service for guaranteed quality, speed, and support:

• Guaranteed Accuracy: All cloned genes are 100% sequence-verified using Next-Generation Sequencing (NGS) and Sanger sequencing to ensure zero errors in the delivered construct.
• Speed & Efficiency: Leveraging high-throughput automation and streamlined processes, we offer industry-competitive turnaround times for both standard and complex sequences.
• Complexity Handling: Expertise in synthesizing challenging sequences, including those with high GC content, tandem repeats, or secondary structures, which are typically difficult for standard providers.
• Free Codon Optimization: Utilize our proprietary, AI-enhanced algorithms to optimize your gene sequence for maximal protein expression in your host system (e.g., E. coli, yeast, mammalian cells, or plants) at no extra cost.

Applications

Synthetic DNA is the critical starting material for numerous applications in life science research and development:

Gene Expression & Protein Engineering

           

Synthesis of codon-optimized genes for high-level expression of recombinant proteins, antibodies, and enzymes for structural and functional studies.

Metabolic & Pathway Engineering

Construction of complex, multi-enzyme gene cassettes and entire biosynthetic pathways for microbial and cellular engineering.

CRISPR & Gene Editing (HDR Templates)

Manufacturing high-purity, long ssDNA (Ultramers) or dsDNA fragments for use as homology-directed repair (HDR) donor templates.

Synthetic Libraries & Mutagenesis

Creation of precisely controlled synthetic variant libraries (random, saturation, trimer) for directed evolution and protein screening.

Products & Solutions

We provide a wide range of DNA products to meet every requirement, from basic PCR to complex pathway construction.

Standard Oligonucleotide Synthesis

Synthesis of short DNA primers and probes (up to ~200 bases) with various purification options (Desalted, HPLC, PAGE) and over 100 modifications.

Gene Fragment Synthesis (gBlocks/eBlocks)

Rapid, cost-effective synthesis of linear dsDNA fragments (e.g., 25 bp to 3 kb) ready for immediate use in cloning (Gibson, Golden Gate, etc.).

Cloned Gene Synthesis (Full-Length Genes)

Synthesis of complex genes (up to 20 kb or more) delivered cloned into a standard or custom client vector with 100% sequence accuracy.

Custom Vector & Plasmid Preparation

Subcloning into client-provided vectors and the preparation of high-quality, endotoxin-free, transfection-grade plasmid DNA (up to milligram scales).

Site-Directed Mutagenesis & DNA Libraries

Precise introduction of point mutations, deletions, or insertions, and the creation of custom, complexity-controlled DNA variant libraries.

Workflow

Our streamlined process ensures efficient and accurate delivery of your synthetic DNA:

• Sequence Submission and Optimization: The client submits the DNA or protein sequence. We perform a technical review and offer free codon optimization based on the target host and expression goals.
• Gene Synthesis & Assembly: Short oligonucleotide building blocks are chemically synthesized. These oligos are then enzymatically assembled via proprietary methods (e.g., PCR-based assembly or ligation-based methods) to create the full-length gene.
• Cloning and Sequence Verification: The assembled gene is cloned into the specified vector (standard or custom). Every clone is rigorously verified by both Sanger sequencing and, for complex regions, Next-Generation Sequencing (NGS) to ensure 100% accuracy.
• Plasmid Preparation and Quality Control: After sequence verification, the positive clone is cultured. We perform plasmid purification, quality check (QC) for concentration and integrity, and offer endotoxin-free preparation as an option.
• Final Delivery: The final product (plasmid DNA, linear fragments, or oligos) is shipped along with a comprehensive data package, including the COA (Certificate of Analysis), vector map, final sequence file, and QC data.

We ensure reliable and high-quality results with every order:

• Zero Error Guarantee: We guarantee 100% sequence accuracy for all cloned genes. If an error is found, we resynthesize the gene at no cost.
• Free Subcloning: We offer complimentary subcloning into our standard in-house vectors, or we can onboard and subclone into your custom vectors.
• Intellectual Property Protection: Strict confidentiality protocols are maintained for all sequence and project data to protect your intellectual property.
• Scalability: Services range from microgram-scale delivery for standard research to milligram or gram-scale for large-scale production or therapeutic applications.