Oligo Pool Synthesis is a cutting-edge technique used in molecular biology to simultaneously synthesize a large number of DNA or RNA sequences. It offers researchers the ability to generate complex pools of oligonucleotides that can be utilized in a wide range of applications, including next-generation sequencing, gene synthesis, and targeted mutagenesis. By synthesizing multiple sequences in parallel, Oligo Pool Synthesis streamlines the experimental process and saves valuable time and resources.
Service Offered
Oligo pool synthesis involves the simultaneous synthesis of multiple oligonucleotides in a single reaction, providing a cost-effective and efficient way to generate diverse sequences. The specific requirements may vary based on the intended application and the complexity of the oligo pool. Advanced instrumentation and automation may be necessary for high-throughput applications such as library construction and screening. Additionally, careful design and validation are crucial to ensuring the functionality and diversity of the synthesized oligo pools. Below is a table outlining the application areas and requirements for oligo pool synthesis:
Oligo Pool Synthesis(TL Schmidt, et al.,2015)
| Application Area |
Description |
Requirements |
| Library Construction |
Generation of diverse libraries for functional genomics or screening purposes. |
Design of oligo pool, DNA synthesizer with the capability for pool synthesis, high-quality reagents, purification methods, sequence verification. |
| High-Throughput Screening |
Creation of pools for large-scale screening assays. |
Design of oligo pool, automation equipment for high-throughput synthesis, quality control measures for pool composition, purification methods. |
| Barcoding |
Incorporation of unique identifiers (barcodes) into oligos for multiplexed experiments. |
Design of barcoded sequences, DNA synthesizer with high-throughput capabilities, quality control for barcode incorporation, purification methods. |
| Phage Display Libraries |
Construction of diverse libraries for phage display experiments. |
Design of oligo pool for phage display, DNA synthesizer with appropriate capabilities, purification methods, compatibility with phage display systems. |
| Functional Genomics |
Synthesis of complex pools for various functional genomics studies. |
Design of oligo pool, DNA synthesizer, purification methods, validation assays for pool functionality. |
Service Process
Our service process is designed to be seamless and efficient, ensuring a smooth experience for our clients. Here is an overview of the steps involved:
- Consultation: We begin by understanding your project requirements. Our team of experts will have a detailed discussion with you to gain insights into your research objectives, pool design preferences, and any specific customization options you may require.
- Pool Design: Based on the information gathered during the consultation phase, our experienced scientists will design a customized oligo pool that meets your research objectives. We take into account factors such as target sequences, desired pool complexity, and any specific modifications or constraints you may have.
- Synthesis: Using cutting-edge technologies and proven methodologies, we synthesize the oligo pool with high efficiency and accuracy. Our experienced technicians ensure that the synthesis process is optimized to achieve the best possible results.
- Quality Control: Quality control is of utmost importance to us. We implement stringent quality control measures to validate the integrity and purity of the oligo pool. This includes rigorous testing for sequence accuracy, length verification, and removal of any impurities or contaminants.
- Delivery: Once the quality control process is complete and the oligo pool passes our strict standards, it is carefully packaged to ensure its integrity during transit. We prioritize timely delivery, and your customized oligo pool will be shipped to you in a secure and timely manner.
Please don't hesitate to reach out to us with any further questions or inquiries. We are committed to providing you with exceptional Oligo Pool Synthesis services and ensuring your research success.
Case Studies
Large-scale normalized oligo pool improved information read-out. (A) Brief workflow for OPN probe mediated oligo pool normalization. Oligos are captured by the corresponding OPN probe and extended to dsDNA by Taq DNA polymerase. Then, the remaining ssDNA oligos and probes are digested by exonuclease I. (B) Structure of oligo for oligo pool normalizing (OPN). The adaptor I region, R (nickase recognition sequence) region, payload, and OPN region. In the OPN region, a barcode sequence with 9 nts in length and a unique “GWSWSWS” pattern with alternating strong (G or C) and weak (A or T) bases (e.g., CACTGT or GTCTGA), by which 256 high specific binding could be generated and universal fragment (17 nts). (C) Normalization of 3′ terminal FAM labeled three oligos, 60, 73, and 90 nts in length, with an input molar ratio of 1:5:25 and the output oligos were quantified wwith a molar ratio of 0.95:1:1.25 on 15% native PAGE after OPN. (D) OPN 1.0 constructed on one anchor sequence with 256 specific target oligo recognition. (E) Lorenz curve showing the cumulative frequency distribution of the oligo pool normalized or not by OPN 1.0. (F) OPN 2.0 constructed from four distinct anchor sequences and one set of 256 barcode with 1024 specific target oligo recognition. (G) Minimal necessary sequencing reads for complete oligos retrieved without dropout for a 254 oligos pool read by PCR and OPN 1.0-iDR, respectively, and a 1024 oligos pool read by PCR and OPN 2.0-iDR, respectively.
Y Gao, et al.,2020
FAQs
Here are some frequently asked questions about our Oligo Pool Synthesis services:
Q: What is the turnaround time for Oligo Pool Synthesis?
A: The turnaround time depends on the complexity and scale of the project. During the consultation phase, our team will provide you with an estimated timeline based on your specific requirements.
Q: Can I customize the composition of the oligo pool?
A: Absolutely! We offer flexible pool design options to meet your specific needs. Our experts will work closely with you to ensure that the composition of the oligo pool aligns perfectly with your research goals.
Q: How can I place an order for Oligo Pool Synthesis?
A: Placing an order is easy. Simply get in touch with our customer support team via phone or email, and they will guide you through the necessary steps to initiate the process. They are friendly, knowledgeable, and always ready to assist you.
Q: What is the minimum order quantity for Oligo Pool Synthesis?
A: We understand that research projects can vary in scale, and we accommodate orders of various sizes. Whether you require a small-scale pool or a large-scale pool, our team will work with you to determine the optimal quantity based on your experimental needs.
Q: What are the storage conditions for the oligo pool?
A: To maintain the stability and integrity of the oligo pool, it is recommended to store it at -20°C. Proper storage conditions will ensure the longevity and usability of the pool for your research needs.
Q: Can I request specific modifications to the oligo pool sequences?
A: Yes, we offer customization options for modifications to the oligo pool sequences. Whether you require specific labeling, conjugation, or other modifications, our team can discuss the possibilities and incorporate them into the pool design.
Q: Are there any limitations on the length of the oligo pool sequences?
A: While we can synthesize oligo pools with a wide range of sequence lengths, there may be practical limitations depending on the complexity and scale of the project. Our team will work closely with you to determine the optimal length that aligns with your research goals.
Q: Do you provide technical support after the delivery of the oligo pool?
A: Yes, we offer ongoing technical support to our clients. If you have any questions or need assistance with the usage or applications of the oligo pool, our knowledgeable support team will be available to provide guidance and address any concerns.
Home
