Synthetic Biology
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Trusted by Leading Research & Pharma Institutions

De Novo Gene Synthesis Services

Transform your research with comprehensive de novo gene synthesis solutions. From sequence design to functional validation, we deliver sequence-perfect synthetic genes with industry-leading turnaround times. Our silicon-based platform enables high-throughput production while maintaining exceptional accuracy.

100% Sequence Accuracy
NGS Verified
Up to 10 kb
Learn More

Trusted by leading research and pharmaceutical institutions

MIT
Pfizer
Stanford
Roche
Johns Hopkins
Merck

Why Choose Us

Sequence-perfect clones guaranteed
Error rate 1:7,500 bp
Up to 10 kb gene synthesis
Express service available

Gene Fragments

300 bp - 5,000 bp, linear dsDNA

Clonal Genes

NGS-verified, cloned into vector

Long Genes

Up to 10 kb, complex constructs

Accuracy Rate
100%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive De Novo Gene Synthesis Solutions

From sequence design to functional delivery, our silicon-based platform enables high-throughput, accurate gene synthesis for any application.

Silicon-Based Synthesis Platform

Our proprietary silicon-based DNA synthesis technology miniaturizes chemical reactions, enabling high-throughput production of thousands of genes while maintaining exceptional accuracy. Each gene undergoes rigorous quality control to ensure sequence perfection.

  • Error rate 1:7,500 bp (gene fragments)
  • NGS-verified clonal genes
  • Up to 10 kb synthesis capability

Industry-Leading Quality Control

Every synthetic gene undergoes comprehensive quality verification. Clonal genes are sequence-verified using next-generation sequencing, ensuring 100% accuracy before delivery. We provide detailed QC reports with every order.

  • 100% sequence accuracy guarantee
  • NGS verification for all clones
  • Detailed QC documentation

Fast Turnaround

Express service delivers genes in as few as 4-7 business days.

Custom Vector Options

Deliver in our catalog vectors or your custom vector design.

Flexible Formats

Gene fragments, clonal genes, or long gene constructs.

Ready to Start Your Project?

Get a customized quote for your de novo gene synthesis needs.

Technology Platform

Advanced Synthesis Technologies

Our silicon-based platform delivers superior accuracy and throughput for gene synthesis.

Silicon-Based Platform

Miniaturized chemical reactions on silicon chips enable high-throughput synthesis with consistent quality across thousands of genes.

High Throughput Consistent Quality

NGS Verification

Every clonal gene undergoes next-generation sequencing verification to ensure 100% sequence accuracy before shipment.

100% Accuracy Complete QC

Flexible Assembly

From gene fragments to long gene constructs up to 10 kb, our platform handles diverse synthesis requirements with precision.

Up to 10 kb Complex Sequences

Quality Control

NGS Next-generation sequencing verification
QC Comprehensive quality checks
COA Certificate of Analysis included
SEQ Sequence data available

Product Options

GF Gene Fragments 300 bp - 5 kb
CG Clonal Genes NGS-verified
LG Long Genes up to 10 kb
CV Custom vector cloning
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter Gene Fragments Clonal Genes Long Genes
Length Range 300 bp - 5,000 bp 300 bp - 5,000 bp 5,000 - 10,000 bp
Error Rate 1:7,500 bp NGS-verified Custom QC
Turnaround Time 4-9 business days 6-12 business days 8-15 business days
Scale Range 100 ng - 1 µg 1 µg - 1 mg 1 µg - 500 µg
Format Linear dsDNA Cloned in vector Cloned in vector
Verification Size/purity check NGS-verified Sequencing
Workflow

Streamlined Process from Design to Delivery

Our proven workflow ensures quality and efficiency at every stage.

1

Order

Submit sequence and specifications

2

Synthesis

Silicon-based platform synthesis

3

QC

NGS verification

4

Packaging

Dried down in tubes or plates

5

Delivery

Fast shipping with QC report

Applications

Diverse Applications Across Biotechnology

Our synthesis services support research and development in multiple fields.

Protein Expression

Synthesize genes optimized for protein expression in various host systems. Our codon optimization service maximizes expression levels while maintaining native protein function.

  • E. coli expression systems
  • Mammalian cell expression
  • Yeast and insect cell systems
  • Codon optimization included
10 kb
Maximum gene length

CRISPR Genome Editing

High-quality synthetic genes for CRISPR applications including donor templates, guide RNA scaffolds, and expression constructs for genome engineering projects.

  • HDR donor templates
  • Cas9 expression constructs
  • Guide RNA scaffolds
  • CRISPR library construction
100%
Sequence accuracy

Synthetic Biology

Build complex genetic circuits and pathways with our high-quality synthetic genes. From single genes to multi-kilobase constructs, we support your synthetic biology research.

  • Gene circuit construction
  • Metabolic pathway engineering
  • Enzyme optimization
  • Biosensor development
1:7,500
Error rate (bp)
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The sequence accuracy is exactly what we needed for our protein expression projects. Every gene we've ordered has expressed correctly without any sequence errors. The NGS verification gives us complete confidence."

S
Senior Scientist
Biopharmaceutical Company

"Fast turnaround times have been critical for our CRISPR library construction. The 4-7 day express service fits perfectly with our project timelines. Customer support is responsive and helpful."

R
Research Director
Academic Research Institution

"We've been using this service for our metabolic engineering projects. The ability to synthesize genes up to 10 kb has been essential for our pathway construction work. Quality is consistently excellent."

P
Principal Investigator
Synthetic Biology Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

85 Citations

The design and engineering of synthetic genomes

James JS, Dai J, Chew WL, Cai Y, et al. Nature Reviews Genetics. 2025.

Comprehensive review of strategies for design, construction, delivery, debugging and tailoring of synthetic genomes as well as their potential applications in bioproduction and cell therapy.

View DOI
42 Citations

De novo gene synthesis by an antiviral reverse transcriptase

Tang S, Conte V, Zhang DJ, Zedaite R, et al. Science. 2024.

Discovery of DRT systems performing DNA synthesis for bacterial defense, revealing concatemeric cDNA products and novel gene creation mechanisms through rolling circle reverse transcription.

View DOI
28 Citations

Semantic design of functional de novo genes from a genomic language model

Groenevelt J, Chen K, Zhou Q, et al. Nature. 2025.

Demonstration that semantic design using genomic language models can generate functional proteins from genomic context alone, creating a database of over 120 billion DNA base pairs.

View DOI
35 Citations

Enzymatic strategies for next-generation DNA synthesis: Boosting efficiency and overcoming secondary structures

Schober S, Krstic I, Guth N, et al. ACS Catalysis. 2025.

Review of enzymatic de novo DNA synthesis as a promising alternative to chemical synthesis, highlighting advances in TdT engineering for improved coupling efficiency.

View DOI
156 Citations

Continuous synthesis of genome sections and Mb-scale human DNA assembly

Wang G, Yamanaka M, Xu Z, et al. Nature. 2023.

Development of BASIS enabling continuous replacement of genomic DNA with synthetic DNA, demonstrated by assembling 1.1 Mb of human DNA with applications for E. coli genome synthesis.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

Gene Fragments typically ship in 4-9 business days. Clonal Genes (NGS-verified) ship in 6-12 business days. Long Genes (up to 10 kb) require 8-15 business days due to increased synthesis complexity. Express service options are available for urgent projects.
All clonal genes undergo next-generation sequencing (NGS) verification to ensure 100% sequence accuracy. Gene fragments are verified through size and purity checks. We provide detailed Certificate of Analysis documentation with every order, including sequencing data.
Yes, our silicon-based platform handles a wide range of sequence complexities including GC-rich regions, repetitive sequences, and challenging secondary structures. Our algorithm evaluates each sequence and applies optimization strategies to maximize synthesis success.
We offer a diverse selection of catalog vectors for cloning and expression, including bacterial, yeast, and mammalian expression vectors. Custom vector onboarding is also available. Clonal genes are delivered ready-to-use in your vector of choice.
Our standard synthesis supports genes up to 5 kb. For long gene constructs, we offer synthesis services up to 10 kb with extended turnaround times and custom quality control protocols to ensure accuracy.
Yes, our service includes codon optimization for your target expression host to maximize protein expression levels. We use multi-parameter optimization considering codon usage, GC content, mRNA secondary structures, and other factors affecting expression.

Ready to Start Your Project?

Get a customized quote for your De Novo Gene Synthesis Service project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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· Antibody gene synthesis service

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
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