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TWIST1 Knockout Cell Lines

Gene: TWIST1

Official Full Name: twist family bHLH transcription factor 1provided by HGNC

Gene Summary: This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Jul 2020]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO09360 TWIST1 Knockout cell line (HeLa) Human TWIST1 1:3~1:6 Negative Online Inquiry
KO09361 TWIST1 Knockout cell line (HCT 116) Human TWIST1 1:2~1:4 Negative Online Inquiry
KO09362 TWIST1 Knockout cell line (HEK293) Human TWIST1 1:3~1:6 Negative Online Inquiry

Background

TWIST1 Gene Knockout Cell Lines are expertly engineered cellular models designed to facilitate the study of the TWIST1 gene's role in various biological processes and diseases. The TWIST1 gene encodes a basic helix-loop-helix transcription factor integral to cellular processes, including epithelial-mesenchymal transition (EMT), development, and tumorigenesis. By utilizing CRISPR-Cas9 technology, these cell lines have been precisely modified to ablate the expression of TWIST1, allowing researchers to investigate the gene's specific functions and multifaceted mechanisms in a controlled environment.

These knockout cell lines serve as vital tools in research aimed at understanding the implications of TWIST1 dysregulation in cancer metastasis, organogenesis, and regenerative medicine. By providing a model that mimics the loss of TWIST1, scientists can elucidate signal transduction pathways, gene interactions, and the resultant phenotypic changes, thus advancing the field of molecular biology and pathology. Additionally, these modified cells can be used in drug screening assays to evaluate therapeutic compounds targeting pathways influenced by TWIST1, thereby promoting the development of novel treatments.

One of the standout advantages of TWIST1 Gene Knockout Cell Lines is their specificity and reliability compared to other general knockout models. The precision of the CRISPR-based approach ensures consistent gene disruption and reduced off-target effects, rendering them ideal for reproducible experimental outcomes. Furthermore, these cell lines are readily adaptable for high-throughput applications, making them an indispensable resource for both academic and pharmaceutical research settings.

For researchers and clinicians keen to deepen their exploration of cellular mechanisms pertaining to TWIST1, our cell lines represent a reliable and advanced solution. Their development is supported by our extensive expertise in genetic engineering and cell culture technologies, ensuring that users receive a superior product tailored to meet the demands of cutting-edge scientific inquiry. With TWIST1 Gene Knockout Cell Lines, you can further enhance your research capabilities and drive impactful discoveries in the biological sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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