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TIAM1 Knockout Cell Lines

Gene: TIAM1

Official Full Name: TIAM Rac1 associated GEF 1provided by HGNC

Gene Summary: This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO08957 TIAM1 Knockout cell line (HeLa) Human TIAM1 1:3~1:6 Negative Online Inquiry
KO08958 TIAM1 Knockout cell line (HCT 116) Human TIAM1 1:2~1:4 Negative Online Inquiry
KO08959 TIAM1 Knockout cell line (HEK293) Human TIAM1 1:3~1:6 Negative Online Inquiry

Background

TIAM1 Gene Knockout Cell Lines are engineered cellular models designed to lack the expression of the TIAM1 gene, a crucial mediator of various cellular processes, including adhesion, migration, and signaling pathways intrinsic to oncogenesis and neurogenesis. By utilizing CRISPR-Cas9 technology, these cell lines facilitate the precise elimination of TIAM1, enabling scientists to dissect its role in cellular dynamics and its involvement in pivotal biological functions.

The primary mechanism of action is based on the knockout approach, wherein the targeted disruption of the TIAM1 gene leads to a cascade of phenotypic changes that can be observed and studied. This allows researchers to investigate the molecular underpinnings of processes such as cell proliferation, cytoskeletal rearrangement, and apoptotic signaling in a controlled environment. Understanding these interactions is essential, particularly in cancer research, where TIAM1's role in tumor metastasis is critically emphasized.

The scientific importance of TIAM1 Gene Knockout Cell Lines is evident in both basic and applied research settings. They serve as invaluable models for studying the mechanisms of cell signaling alterations in a variety of diseases, particularly those involving aberrant cellular migration and invasion. Additionally, these cell lines are integral in drug discovery and development, helping identify potential therapeutic targets and assess drug efficacy.

Compared to traditional cell line models, TIAM1 Gene Knockout Cell Lines offer distinct advantages, including highly reproducible results, controlled experimental conditions, and the capacity to model disease states more accurately. Unlike alternative knockout strategies that may have off-target effects, our cell lines ensure specificity due to the meticulous design of the CRISPR system.

For researchers and clinicians dedicated to unraveling the complexities of cellular behavior in health and disease, TIAM1 Gene Knockout Cell Lines present an exceptional tool that enhances experimental rigor and promotes innovative insights. Our deep expertise in molecular biology and commitment to providing high-quality biological products ensure that you receive reliable tools essential for advancing your research.

Please note that all services are for research use only. Not intended for any clinical use.

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