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RTRAF Knockout Cell Lines

Gene: RTRAF

Official Full Name: RNA transcription, translation and transport factorprovided by HGNC

Gene Summary: Enables RNA binding activity; RNA polymerase II complex binding activity; and identical protein binding activity. Involved in negative regulation of protein kinase activity; positive regulation of transcription by RNA polymerase II; and tRNA splicing, via endonucleolytic cleavage and ligation. Located in microtubule cytoskeleton; nucleoplasm; and perinuclear region of cytoplasm. Part of tRNA-splicing ligase complex. [provided by Alliance of Genome Resources, Apr 2025]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO22340 RTRAF Knockout cell line (HeLa) Human RTRAF 1:3~1:6 Negative Online Inquiry
KO22341 RTRAF Knockout cell line (HCT 116) Human RTRAF 1:2~1:4 Negative Online Inquiry
KO22342 RTRAF Knockout cell line (HEK293) Human RTRAF 1:3~1:6 Negative Online Inquiry
KO22343 RTRAF Knockout cell line (A549) Human RTRAF 1:3~1:4 Negative Online Inquiry

Background

RTRAF Gene Knockout Cell Lines are advanced biological tools engineered for the targeted ablation of the RTRAF gene, enabling researchers to study gene function and its implications in various biological processes. These cell lines utilize CRISPR-Cas9 technology to create precise deletions or modifications in the gene coding for the RTRAF protein, a critical regulator involved in immune responses and cell signaling pathways. By eliminating the RTRAF gene, researchers can investigate the resulting phenotypic changes, providing valuable insights into gene function, signaling cascades, and potential therapeutic targets.

The key mechanism behind these knockout cell lines lies in the precise editing capabilities of the CRISPR-Cas9 system, which employs a guide RNA to direct the Cas9 nuclease to the specific DNA sequence of the RTRAF gene. Upon binding, Cas9 introduces double-strand breaks, leading to knockout through endogenous repair processes. This targeted approach yields a more robust and reproducible reduction of RTRAF expression compared to conventional methods, ensuring higher specificity and reduced off-target effects.

Scientifically, RTRAF Gene Knockout Cell Lines are invaluable in both research and clinical settings. Their applications span from elucidating molecular mechanisms underlying autoimmune diseases to screening novel drug candidates that can modulate RTRAF-associated pathways. As researchers and clinicians navigate the complexities of gene regulation in health and disease, these cell lines serve as a versatile platform for both foundational and translational research.

What sets RTRAF Gene Knockout Cell Lines apart from alternative offerings is the rigorous validation of gene knockout efficiency and stability, alongside comprehensive user support that helps facilitate experimental design and implementation. Additionally, these cell lines are readily available in multiple backgrounds, catering to a wide array of experimental needs and ensuring compatibility with diverse research protocols.

For researchers and clinicians aiming to deepen their understanding of gene function and develop innovative therapeutic strategies, RTRAF Gene Knockout Cell Lines represent a cutting-edge solution backed by our company's commitment to scientific excellence and innovation in the realm of genetic research tools. Our expertise in biological product development ensures that customers receive high-quality, reliable, and effective research solutions tailored to their unique project requirements.

Please note that all services are for research use only. Not intended for any clinical use.

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