Gene: RFFL
Official Full Name: ring finger and FYVE like domain containing E3 ubiquitin protein ligaseprovided by HGNC
Gene Summary: Enables enzyme binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in several processes, including negative regulation of cysteine-type endopeptidase activity; negative regulation of signal transduction; and protein K48-linked ubiquitination. Located in endosome membrane and plasma membrane. [provided by Alliance of Genome Resources, Apr 2025]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO30432 | RFFL Knockout cell line (HeLa) | Human | RFFL | 1:3~1:6 | Negative | Online Inquiry |
KO30433 | RFFL Knockout cell line (HCT 116) | Human | RFFL | 1:2~1:4 | Negative | Online Inquiry |
KO30434 | RFFL Knockout cell line (HEK293) | Human | RFFL | 1:3~1:6 | Negative | Online Inquiry |
KO30435 | RFFL Knockout cell line (A549) | Human | RFFL | 1:3~1:4 | Negative | Online Inquiry |
RFFL Gene Knockout Cell Lines are genetically modified cell lines specifically designed to functionally analyze the roles and implications of the RFFL gene in cellular processes. These knockout cell lines are engineered using CRISPR-Cas9 technology, which enables precise modification of the genome, resulting in the complete ablation of the RFFL gene expression. By creating these knockout cell lines, researchers can elucidate the biological functions of RFFL, a gene implicated in various pathways including apoptosis and immune response, ultimately contributing to a deeper understanding of important metabolic and signaling networks.
The key mechanism of action of these cell lines lies in the absence of RFFL protein, allowing for comparative studies against wild-type controls. This enables researchers to identify changes in cellular behavior, gene expression profiles, and sensitivity to various stimuli or drugs. Such insights are crucial in both academic research and clinical applications, particularly for developing targeted therapies in cases where RFFL may play a pivotal role in pathophysiology, such as certain cancers and autoimmune diseases.
One of the significant advantages of RFFL Gene Knockout Cell Lines over traditional methods of gene deletion is the precision and efficiency achieved through CRISPR technology. These cell lines provide a stable and reproducible model for experiments, minimizing variability and enhancing data reliability. Additionally, they save valuable time and resources as researchers can focus directly on studying the functional repercussions of the RFFL knockout without the need for extensive validation typical of other methods.
For researchers and clinicians navigating the complexities of gene function and disease, RFFL Gene Knockout Cell Lines represent an invaluable tool, providing a robust platform to test hypotheses and explore therapeutic avenues. With a commitment to cutting-edge biotechnological innovations, our company offers these cell lines as part of a comprehensive portfolio aimed at advancing biological research and contributing to medical breakthroughs. Our expertise in genetic engineering ensures that each product is of the highest quality, supporting our clients in their quest for scientific excellence.
Please note that all services are for research use only. Not intended for any clinical use.
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