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PYGL Knockout Cell Lines

Gene: PYGL

Official Full Name: glycogen phosphorylase Lprovided by HGNC

Gene Summary: This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO35745 PYGL Knockout cell line (HeLa) Human PYGL 1:3~1:6 Negative Online Inquiry
KO35746 PYGL Knockout cell line (HCT 116) Human PYGL 1:2~1:4 Negative Online Inquiry
KO35747 PYGL Knockout cell line (HEK293) Human PYGL 1:3~1:6 Negative Online Inquiry
KO35748 PYGL Knockout cell line (A549) Human PYGL 1:3~1:4 Negative Online Inquiry

Background

PYGL Gene Knockout Cell Lines are specialized cell lines engineered to lack the expression of the PYGL gene, which encodes for the enzyme glycogen phosphorylase, a critical enzyme involved in glycogen metabolism. These knockout models are invaluable tools for studying the physiological and pathological roles of glycogen catabolism, particularly in conditions such as diabetes, obesity, and muscle disorders.

The key function of these cell lines is to enable researchers to explore the biochemical and molecular consequences of compromised glycogen breakdown. The absence of the PYGL gene allows for the investigation of cellular responses, metabolic alterations, and adaptive mechanisms that occur when glycogen mobilization is hindered. By employing techniques such as glucose tolerance tests, insulin sensitivity assessments, and metabolic profiling, researchers can elucidate how the PYGL gene impacts energy metabolism and overall cellular function.

In the context of scientific importance, PYGL Gene Knockout Cell Lines offer a robust platform for drug discovery and development, particularly in assessing therapeutic targets aimed at metabolic diseases. With the growing prevalence of metabolic disorders worldwide, understanding the dysregulation of glycogen metabolism is critical, and these cell lines serve as an essential resource for advancing our knowledge.

Compared to traditional cell lines, PYGL Gene Knockout Cell Lines provide a unique benefit by specifically simulating disease conditions that are otherwise difficult to model accurately. This specificity enhances the reliability of experimental outcomes, allowing for better predictions of in vivo responses.

For researchers and clinicians dedicated to unraveling metabolic pathways and developing effective treatments, PYGL Gene Knockout Cell Lines deliver significant value. They cater to the need for precision tools in precise metabolic research, ultimately advancing both basic science and clinical applications.

At [Your Company Name], we pride ourselves on our commitment to providing high-quality biological products that empower research excellence. Our expertise in gene editing technologies ensures that our offerings, including PYGL Gene Knockout Cell Lines, are among the finest available, supporting your groundbreaking studies in metabolism and beyond.

Please note that all services are for research use only. Not intended for any clinical use.

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