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PLOD1 Knockout Cell Lines

Gene: PLOD1

Official Full Name: procollagen-lysine,2-oxoglutarate 5-dioxygenase 1provided by HGNC

Gene Summary: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
GP00707 PLOD1 gRNA1-gRNA2 KO plasmid PLOD1 $850
GP00708 PLOD1 gRNA3-gRNA4 KO plasmid PLOD1 $850
KO03747 PLOD1 Knockout cell line (HeLa) Human PLOD1 1:3~1:6 Negative Online Inquiry
KO03748 PLOD1 Knockout cell line (HCT 116) Human PLOD1 1:2~1:4 Negative Online Inquiry
KO03749 PLOD1 Knockout cell line (HEK293) Human PLOD1 1:3~1:6 Negative Online Inquiry
KO03750 PLOD1 Knockout cell line (A549) Human PLOD1 1:3~1:4 Negative Online Inquiry

Background

PLOD1 Gene Knockout Cell Lines are genetically engineered cell lines in which the PLOD1 gene has been selectively disrupted to explore the biological implications of its absence. This innovative tool is indispensable for researchers investigating the role of PLOD1, which encodes prolyl 4-hydroxylase, an enzyme critical for collagen synthesis and extracellular matrix stability. The knockout of this gene allows for a deeper understanding of the mechanistic pathways involved in fibrotic diseases, wound healing, and various connective tissue disorders.

These knockout cell lines function by providing a controlled environment to elucidate the specific pathways affected when PLOD1 is non-functional. Researchers can study the resulting changes in collagen deposition, cellular signaling pathways, and overall cellular phenotype, allowing for insights into the pathophysiology of diseases linked to abnormal collagen production. The versatility of these cell lines makes them suitable for various applications, including drug discovery, gene therapy exploration, and the investigation of tissue engineering strategies.

The scientific importance of PLOD1 Gene Knockout Cell Lines cannot be overstated. They facilitate the development of novel therapeutic approaches to combat diseases characterized by excessive fibrosis or impaired wound healing. Compared to traditional cell lines, these knockout models provide a more relevant framework for research by reflecting the impact of PLOD1 loss on cell function and behavior. Furthermore, the specificity of these models allows for objective assessment of treatment efficacy in preclinical studies, enhancing the translational potential of findings.

For researchers and clinicians, PLOD1 Gene Knockout Cell Lines offer unparalleled value by providing a unique avenue to address important biomedical questions. Their ease of use, combined with robust performance metrics, ensure consistent and reproducible results that are vital for both exploratory and applied research.

As a leader in the field of gene-editing technologies, our company is dedicated to providing high-quality biological products that empower scientific discovery. With a commitment to innovation and excellence, we support the research community in unlocking new frontiers in molecular biology and medicine. Choose PLOD1 Gene Knockout Cell Lines to enhance your research endeavors and drive impactful discoveries in health and disease.

Please note that all services are for research use only. Not intended for any clinical use.

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