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PCSK4 Knockout Cell Lines

Gene: PCSK4

Official Full Name: proprotein convertase subtilisin/kexin type 4provided by HGNC

Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. The protease is expressed only in the testis, placenta, and ovary. It plays a critical role in fertilization, fetoplacental growth, and embryonic development and processes multiple prohormones including pro-pituitary adenylate cyclase-activating protein and pro-insulin-like growth factor II. [provided by RefSeq, Jan 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
GP00521 PCSK4 gRNA1-gRNA2 KO plasmid PCSK4 $850
KO00825 PCSK4 Knockout cell line(HCT 116) Human PCSK4 1:2~1:4 Negative Online Inquiry
KO02536 PCSK4 Knockout cell line (HeLa) Human PCSK4 1:3~1:6 Negative Online Inquiry
KO02537 PCSK4 Knockout cell line (A549) Human PCSK4 1:3~1:4 Negative Online Inquiry

Background

PCSK4 Gene Knockout Cell Lines represent a state-of-the-art tool designed for the study of the proprotein convertase subtilisin/kexin type 4 (PCSK4) gene, which plays a crucial role in various biological processes, including fertilization and the development of specific tissues. These gene knockout cell lines are engineered to disrupt the PCSK4 gene, allowing researchers to investigate the functional implications of its absence.

The primary mechanism of these cell lines involves the targeted deletion of the PCSK4 gene, achieved through the application of CRISPR-Cas9 technology. This precision gene-editing technique ensures a high efficiency of knockout events, leading to a stable line that facilitates the observation of cellular and molecular outcomes attributable to PCSK4 loss. Researchers can utilize these cell lines to study altered signaling pathways, changes in protein expression levels, and the impacts on cellular behavior, offering insights into pathological conditions and potential therapeutic targets.

The scientific importance of PCSK4 Gene Knockout Cell Lines cannot be overstated. These models are invaluable in both fundamental research and clinical settings, particularly in the fields of reproductive biology, oncology, and cardiovascular studies. The ability to elucidate the role of PCSK4 in diseases provides a pathway for developing novel interventions and understanding key biological mechanisms.

Compared to alternative models, such as wild-type cell lines or less targeted gene editing techniques, the PCSK4 Gene Knockout Cell Lines offer specificity and reliability. Researchers gain direct access to a unique genetic alteration, allowing for clearer interpretation of experimental results and faster advancements in understanding gene function.

Ultimately, the value of this product lies in its ability to empower researchers and clinicians to delve deeper into the functional aspects of PCSK4, leading towards innovative discoveries that can influence both diagnostics and therapeutic strategies. Our empresa is dedicated to providing high-quality biological products backed by extensive expertise, ensuring that researchers have the best tools available to advance their work.

Please note that all services are for research use only. Not intended for any clinical use.

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