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PC Knockout Cell Lines

Gene: PC

Official Full Name: pyruvate carboxylaseprovided by HGNC

Gene Summary: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO00371 PC Knockout cell line(HEK293) Human PC 1:3~1:6 Negative Online Inquiry
KO06409 PC Knockout cell line (HeLa) Human PC 1:3~1:6 Negative Online Inquiry
KO06410 PC Knockout cell line (HCT 116) Human PC 1:2~1:4 Negative Online Inquiry
KO06411 PC Knockout cell line (A549) Human PC 1:3~1:4 Negative Online Inquiry

Background

PC Gene Knockout Cell Lines are expertly engineered cellular models designed to facilitate the study of gene function by providing researchers with the ability to create targeted gene deletions. These cell lines utilize CRISPR-Cas9 technology or other gene-editing techniques to induce precise mutations, effectively knocking out specific genes of interest. This functionality allows for the in-depth analysis of gene roles in various biological processes, including development, disease mechanisms, and cellular metabolism.

The primary mechanism by which PC Gene Knockout Cell Lines operate involves the targeted delivery of CRISPR reagents, which are designed to introduce double-strand breaks in the genomic DNA at specific loci. When the cell attempts to repair these breaks through non-homologous end joining, random insertions or deletions occur, leading to gene inactivation. This process enables researchers to observe phenotypic changes, elucidate pathways, and validate potential therapeutic targets.

The scientific significance of these cell lines is profound, fostering advancements in genetic research, drug discovery, and the development of precision medicine. Their applications extend to various fields, including cancer research, developmental biology, and regenerative therapies—offering a robust platform for unraveling the complexities of gene function in both in vitro and in vivo settings.

One of the standout advantages of PC Gene Knockout Cell Lines lies in their versatility and high efficiency in gene editing compared to traditional methods such as RNA interference or random mutagenesis, which may yield inconsistent or off-target effects. This precision leads to more reliable data, allowing researchers to make informed decisions when progressing from hypothesis to experimental validation.

For researchers and clinicians, the ability to utilize these advanced cell lines translates into significant time and cost savings in experimental design and execution, enhancing the likelihood of successful outcomes in project development. With years of expertise in cellular engineering, our company is dedicated to delivering high-quality biological tools that empower scientific discovery and innovation, ensuring that you are equipped with the most effective resources for your research endeavors.

Please note that all services are for research use only. Not intended for any clinical use.

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