Mirlet7f-1 Knockout Cell Lines

Gene: Mirlet7f-1

Official Full Name: microRNA let7f-1provided by MGI

Gene Summary: microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO01178	Mirlet7f-1 Knockout cell line(RAW 264.7)	Mouse	Mirlet7f-1	1:2-1:3	Negative	Online Inquiry

Background

Mirlet7f-1 Gene Knockout Cell Lines represent a crucial advancement in molecular biology, specifically designed for research focused on gene function and regulation. These cell lines feature a targeted knockout of the mirlet7f-1 gene, allowing researchers to investigate the roles of this microRNA in various biological processes, including cell differentiation, proliferation, and apoptosis.

The mechanism underlying the functionality of the Mirlet7f-1 knockout involves the deliberate disruption of the mirlet7f-1 locus through cutting-edge gene-editing technologies such as CRISPR/Cas9 or related methodologies. By eliminating this microRNA, the cell lines enable the detailed study of its downstream targets and pathways, thereby elucidating its contributions to cellular behavior and disease states such as cancer or metabolic disorders.

One of the key scientific significance of these cell lines lies in their application for functional genomics and drug discovery. Researchers can employ them to model specific diseases, identify novel therapeutic targets, and assess the efficacy of potential drugs in environments devoid of the mirlet7f-1 regulatory influence. This adaptability makes the Mirlet7f-1 knockout cell lines invaluable in both academic and clinical research settings, aiding in the advancement of personalized medicine strategies.

Compared to alternatives, these knockout cell lines offer a controlled model system with predictable genetic background, reducing variability and enhancing reproducibility in experiments. Additionally, they are engineered to provide high efficacy, ensuring that researchers achieve consistent results with minimal off-target effects.

For investigators and clinicians seeking to understand microRNA biology and its implications in health and disease, Mirlet7f-1 Gene Knockout Cell Lines are an essential resource. They not only enhance our understanding of genetic regulation but also foster the development of innovative therapeutic interventions. With our company’s commitment to high-quality biological products and extensive expertise in the field, we empower researchers to push the boundaries of scientific discovery.

Please note that all services are for research use only. Not intended for any clinical use.

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