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LAMC1 Knockout Cell Lines

Gene: LAMC1

Official Full Name: laminin subunit gamma 1provided by HGNC

Gene Summary: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO00624 LAMC1 Knockout cell line(HCT 116) Human LAMC1 1:2~1:4 Negative Online Inquiry
KO10247 LAMC1 Knockout cell line (HeLa) Human LAMC1 1:3~1:6 Negative Online Inquiry
KO10248 LAMC1 Knockout cell line (HEK293) Human LAMC1 1:3~1:6 Negative Online Inquiry
KO10249 LAMC1 Knockout cell line (A549) Human LAMC1 1:3~1:4 Negative Online Inquiry

Background

LAMC1 Gene Knockout Cell Lines are meticulously engineered cellular systems designed to facilitate the study of the LAMC1 gene, which encodes the lambda-1 chain of laminin, a critical component of the extracellular matrix. These knockout cell lines have been created using state-of-the-art CRISPR-Cas9 technology, resulting in the precise deletion of the LAMC1 gene within the host cells. This gene's functions are integral to various cell behaviors including adhesion, migration, and tissue morphogenesis.

The primary mechanism of action for these knockout cell lines is their ability to provide researchers with an in-depth understanding of laminin's role in tumorigenesis, angiogenesis, and cellular interactions within the microenvironment. By examining the phenotypic changes exhibited by these cells in the absence of LAMC1, researchers can elucidate its specific contributions to disease states, especially in cancer biology and regenerative medicine. This model is particularly valuable in deciphering the complex signaling pathways that dictate cell behavior in both physiological and pathological contexts.

The scientific importance of LAMC1 Gene Knockout Cell Lines extends beyond basic research; they are essential tools in drug discovery and the development of therapeutic strategies. Researchers can utilize these lines to screen potential anti-cancer compounds, assess drug efficacy, or explore the effects of altered cellular environments on cell function.

Compared to traditional wild-type cell lines, the knockout lines offer enhanced specificity and accuracy in experimental results, allowing for more reliable data interpretation and reproducibility. This specificity can lead to time and resource savings in both research and clinical applications, making these knockout lines a superior choice for studies focused on extracellular matrix interactions and cancer research.

In selecting LAMC1 Gene Knockout Cell Lines, researchers and clinicians gain the chance to leverage cutting-edge genetic engineering to uncover novel insights into cell biology and disease mechanisms. The valuable information gleaned from these studies can ultimately pave the way for the development of targeted therapies and improved patient outcomes.

Our company prides itself on its commitment to advancing biological research. We specialize in the development of high-quality genetic tools and model systems that empower scientists to explore the frontiers of cellular and molecular biology, ensuring that your research is supported by the most innovative and reliable products available.

Please note that all services are for research use only. Not intended for any clinical use.

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