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GCAT Knockout Cell Lines

Gene: GCAT

Official Full Name: glycine C-acetyltransferaseprovided by HGNC

Gene Summary: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO29276 GCAT Knockout cell line (HeLa) Human GCAT 1:3~1:6 Negative Online Inquiry
KO29277 GCAT Knockout cell line (HCT 116) Human GCAT 1:2~1:4 Negative Online Inquiry
KO29278 GCAT Knockout cell line (HEK293) Human GCAT 1:3~1:6 Negative Online Inquiry
KO29279 GCAT Knockout cell line (A549) Human GCAT 1:3~1:4 Negative Online Inquiry

Background

GCAT Gene Knockout Cell Lines are specialized cellular models designed for gene function studies, allowing researchers to systematically investigate the roles of targeted genes through the absence of their expression. These cell lines are meticulously engineered using advanced CRISPR/Cas9 technology, which enables precise and efficient gene editing. By knocking out specific genes, researchers can observe the resultant phenotypic changes and elucidate molecular pathways, thereby offering deep insights into gene function, regulation, and interactions.

The key mechanism at work in GCAT Gene Knockout Cell Lines relies on the intentional introduction of targeted double-strand breaks in the genomic DNA, which is subsequently repaired through non-homologous end joining—the process that can introduce mutations leading to complete gene knockout. This model has broad applications across various fields, including cancer research, pharmacology, and genetic disease studies, positioning it as a pivotal tool for both academic and applied research settings.

One significant advantage of GCAT Gene Knockout Cell Lines is their consistency and reliability, as they provide well-characterized genetic backgrounds that facilitate reproducibility in experiments. Furthermore, the versatility of these cell lines enables high-throughput screening, which is extremely valuable for drug discovery and functional genomics studies. Compared to other gene alteration methodologies, the CRISPR-based approach used in GCAT highlights not only precision but also efficiency, which significantly reduces resources and time needed for experimental setups.

By integrating GCAT Gene Knockout Cell Lines into research workflows, scientists and clinicians can accelerate their discoveries and advance therapeutic strategies. The ability to visualize the absence of gene function and its biological repercussions in a controlled environment is invaluable for driving the development of new treatments and understanding complex diseases.

At [Your Company Name], we bring decades of biotechnology expertise into the development of high-quality biological products like GCAT Gene Knockout Cell Lines, ensuring that researchers have access to the best tools to drive their scientific investigations forward.

Please note that all services are for research use only. Not intended for any clinical use.

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