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DOCK3 Knockout Cell Lines

Gene: DOCK3

Official Full Name: dedicator of cytokinesis 3provided by HGNC

Gene Summary: This gene is specifically expressed in the central nervous system (CNS). It encodes a member of the DOCK (dedicator of cytokinesis) family of guanine nucleotide exchange factors (GEFs). This protein, dedicator of cytokinesis 3 (DOCK3), is also known as modifier of cell adhesion (MOCA) and presenilin-binding protein (PBP). The DOCK3 and DOCK1, -2 and -4 share several conserved amino acids in their DHR-2 (DOCK homology region 2) domains that are required for GEF activity, and bind directly to WAVE proteins [Wiskott-Aldrich syndrome protein (WASP) family Verprolin-homologous proteins] via their DHR-1 domains. The DOCK3 induces axonal outgrowth in CNS by stimulating membrane recruitment of the WAVE complex and activating the small G protein Rac1. This gene is associated with an attention deficit hyperactivity disorder-like phenotype by a complex chromosomal rearrangement. [provided by RefSeq, Aug 2010]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO38189 DOCK3 Knockout cell line (HCT 116) Human DOCK3 1:2~1:4 Negative Online Inquiry
KO38190 DOCK3 Knockout cell line (HEK293) Human DOCK3 1:3~1:6 Negative Online Inquiry

Background

DOCK3 Gene Knockout Cell Lines are specifically engineered cellular models designed to investigate the biological roles and mechanisms of the DOCK3 gene, a member of the Dedicator of Cytokinesis (DOCK) protein family known for its involvement in cytoskeletal reorganization and cell signaling pathways. This product enables researchers to study the function of DOCK3 by providing a cellular environment devoid of its expression, thus facilitating a deeper understanding of its contributions to various physiological and pathological processes.

The knockout mechanism employed in these cell lines utilizes CRISPR/Cas9 technology, allowing for precise gene editing that results in a complete disruption of the DOCK3 gene. This precise alteration induces phenotypic changes that can be systematically analyzed to elucidate DOCK3’s role in processes such as cell proliferation, migration, and differentiation. By providing a controlled setting to observe the outcomes of gene loss, researchers can unravel complex signaling networks, providing valuable insights into the pathogenesis of diseases where DOCK3 is implicated, such as cancer and neurological disorders.

The scientific importance of DOCK3 Gene Knockout Cell Lines is profound, as they offer a robust platform for drug discovery and the development of targeted therapeutics in both research and clinical settings. Unlike traditional models that may exhibit compensatory pathways, these knockout lines provide clarity and reliability in results regarding the specific effects of DOCK3 absence on cellular behaviors.

One of the unique selling points of our DOCK3 Gene Knockout Cell Lines is the rigorous validation process, which ensures the complete knockout and functional characterization of the cell lines, unlike others in the market that may suffer from incomplete edits or variabilities. Additionally, these cell lines are compatible with various assays, making them ideal for advancing both basic and translational research.

In summary, DOCK3 Gene Knockout Cell Lines are an invaluable resource for researchers and clinicians aiming to explore the intricacies of cellular dynamics influenced by the DOCK3 gene. Our company’s commitment to quality and innovation positions us as a leader in providing high-fidelity gene knockout models, empowering scientific discovery and medical advancements.

Please note that all services are for research use only. Not intended for any clinical use.

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