Gene: CCDC115
Official Full Name: vacuolar ATPase assembly factor VMA22provided by HGNC
Gene Summary: The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) vesicles in some human cells. The encoded protein shares some homology with the yeast V-ATPase assembly factor Vma22p, and the orthologous protein in mouse promotes cell proliferation and suppresses cell death. Defects in this gene are a cause of congenital disorder of glycosylation, type IIo in humans. [provided by RefSeq, Mar 2016]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO01091 | Ccdc115 Knockout cell line (RAW264.7) | Mouse | Ccdc115 | 1:2-1:3 | Negative | Online Inquiry |
CCDC115 Gene Knockout Cell Lines are specifically engineered cellular models designed for the comprehensive study of the CCDC115 gene, which is implicated in various cellular processes and diseases. These cell lines are generated using advanced CRISPR-Cas9 gene-editing technology that facilitates the precise deletion or disruption of the CCDC115 gene, allowing researchers to investigate the functional consequences of its loss.
The key mechanism behind these knockout cell lines relies on the CRISPR-Cas9 system, where guide RNA directs the Cas9 nuclease to the targeted gene sequence within the genome. This results in a double-strand break that, when repaired by the cell's natural DNA repair machinery, leads to insertions or deletions that disrupt gene function. Consequently, CCDC115 Gene Knockout Cell Lines provide invaluable insights into the gene's role in pathways such as cellular proliferation, migration, and disease progression, making them indispensable for research into conditions like primary ciliary dyskinesia and other related disorders.
In the context of scientific research and clinical applications, these knockout cell lines serve as critical tools for gene function studies, drug discovery, and validation of therapeutic targets. Their availability facilitates the exploration of disease mechanisms in a controlled environment, enhancing the reproducibility of experiments and the reliability of results.
One of the primary advantages of CCDC115 Gene Knockout Cell Lines is their high specificity and efficiency compared to traditional gene silencing methods such as RNA interference (RNAi). While RNAi can lead to off-target effects and incomplete gene silencing, CRISPR-Cas9 knockout allows for a more definitive assessment of gene function by eliminating gene expression entirely. Furthermore, our cell lines are thoroughly characterized for background mutations, ensuring that any observed phenotypic changes can be attributed directly to the manipulation of the CCDC115 gene.
For researchers and clinicians, the CCDC115 Gene Knockout Cell Lines not only provide a robust and reliable model for functional genomics but also serve as a springboard for innovation in therapeutic development and personalized medicine. By investing in these knockout models, users can streamline their investigations into genetic functions and potential treatments, enhancing the pace of discovery.
As pioneers in the field of genetic cell line production, our company is committed to supplying high-quality biological products that empower scientists in their research endeavors. We pride ourselves on our extensive expertise and the rigor of our methodologies, ensuring that you receive reliable, cutting-edge tools for your scientific pursuits.
Please note that all services are for research use only. Not intended for any clinical use.
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