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CMBL Knockout Cell Lines

Gene: CMBL

Official Full Name: carboxymethylenebutenolidase homologprovided by HGNC

Gene Summary: CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO26464 CMBL Knockout cell line (HeLa) Human CMBL 1:3~1:6 Negative Online Inquiry
KO26465 CMBL Knockout cell line (HCT 116) Human CMBL 1:2~1:4 Negative Online Inquiry
KO26466 CMBL Knockout cell line (HEK293) Human CMBL 1:3~1:6 Negative Online Inquiry
KO26467 CMBL Knockout cell line (A549) Human CMBL 1:3~1:4 Negative Online Inquiry

Background

CMBL Gene Knockout Cell Lines are sophisticated research tools designed specifically to investigate the functionalities of specific genes by completely disabling their expression. These genetically modified cell lines utilize the CRISPR-Cas9 gene-editing technology, which allows for precise alterations in the genomic sequences of targeted cells. Through the introduction of site-specific double-strand breaks, the CRISPR system facilitates the natural repair mechanisms of the host cell, ultimately leading to the inactivation of the gene of interest. This mechanism of action ensures a high level of specificity and efficiency in gene modification, thereby enabling thorough studies on gene function, interaction, and regulatory pathways.

The scientific importance of CMBL Gene Knockout Cell Lines is underscored by their wide-ranging applications in both basic and applied research settings. They are indispensable tools for researchers investigating gene roles in cellular processes, disease models, drug discovery, and therapeutic developments. By utilizing these knockout lines, researchers can elucidate pathways involved in oncogenesis, metabolism, and numerous other biological phenomena, thereby contributing to advancements in personalized medicine and novel therapeutic strategies.

Compared to traditional gene silencing methods, such as RNAi, CMBL Gene Knockout Cell Lines offer distinct advantages, including permanent gene disruption and a reduction in off-target effects. This leads to more reliable data generation while saving time and resources during experimental setups. Furthermore, the availability of a diverse range of knockout lines covering numerous genes significantly enhances the potential for researchers to achieve comprehensive insights into genetic and biochemical pathways.

The value of CMBL Gene Knockout Cell Lines lies not only in their precision and reliability but also in their ability to advance the frontiers of genetic research. They empower researchers and clinicians to uncover novel targets for intervention and deepen our understanding of complex biological systems. Backed by our company’s extensive expertise in genetic engineering and commitment to quality, CMBL provides researchers with state-of-the-art tools that facilitate impactful discoveries in the field of molecular biology and beyond.

Please note that all services are for research use only. Not intended for any clinical use.

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