CACNA1A Knockout Cell Lines

Gene: CACNA1A

Official Full Name: calcium voltage-gated channel subunit alpha1 Aprovided by HGNC

Gene Summary: Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants encoding different isoforms have been found for this gene. In one set of transcript variants, the (CAG)n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-18 to 21-33 in the coding region is associated with spinocerebellar ataxia 6. [provided by RefSeq, Jul 2016]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO10923	CACNA1A Knockout cell line (HCT 116)	Human	CACNA1A	1:2~1:4	Negative	Online Inquiry

Background

CACNA1A Gene Knockout Cell Lines represent a groundbreaking advancement in cellular biology, designed to facilitate in-depth research into the functions and roles of the CACNA1A gene, which encodes the alpha-1A subunit of voltage-gated calcium channels. These specialized cell lines are genetically engineered to eliminate the expression of the CACNA1A gene, allowing researchers to explore the resulting phenotypic outcomes and physiological processes in a controlled environment.

The key function of these knockout cell lines lies in their ability to mimic the pathophysiological aspects of various neurological disorders associated with dysregulated calcium signaling, including ataxia and migraine. By utilizing CRISPR-Cas9 technology for precise gene editing, the knockout cell lines act as vital tools for studying calcium channel function, intracellular signaling pathways, and neurotransmitter release mechanisms.

Scientifically, CACNA1A gene knockout cell lines are invaluable for both fundamental research and clinical applications. They enable researchers to investigate the molecular basis of calcium dysregulation in diseases, assess the effects of potential drug candidates on calcium signaling pathways, and develop targeted therapies that could ameliorate symptoms or alter disease progression.

Unlike traditional models, these cell lines offer enhanced specificity, reproducibility, and a simplified environment to isolate the effects of the CACNA1A gene deletion. The ability to work with a standardized knockout model accelerates research timelines and improves data reliability, making it an indispensable resource in both academic and pharmaceutical research settings.

For researchers and clinicians focused on understanding calcium channelopathies and creating innovative therapeutic strategies, CACNA1A Gene Knockout Cell Lines serve as a critical resource. Our company specializes in providing high-quality, genetically engineered models backed by extensive validation data, ensuring users access robust and reliable tools to advance their research endeavors. With cutting-edge technology and a commitment to scientific excellence, we are dedicated to supporting the scientific community in unlocking the complexities of calcium signaling in health and disease.

Please note that all services are for research use only. Not intended for any clinical use.

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