Gene: ASAP2
Official Full Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2provided by HGNC
Gene Summary: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO33785 | ASAP2 Knockout cell line (HeLa) | Human | ASAP2 | 1:3~1:6 | Negative | Online Inquiry |
KO33786 | ASAP2 Knockout cell line (HCT 116) | Human | ASAP2 | 1:2~1:4 | Negative | Online Inquiry |
KO33787 | ASAP2 Knockout cell line (HEK293) | Human | ASAP2 | 1:3~1:6 | Negative | Online Inquiry |
KO33788 | ASAP2 Knockout cell line (A549) | Human | ASAP2 | 1:3~1:4 | Negative | Online Inquiry |
ASAP2 Gene Knockout Cell Lines are specialized cellular models designed to facilitate the functional analysis of the ASAP2 gene, which encodes a member of the N-WASP family of proteins involved in actin cytoskeleton dynamics. These knockout cell lines are engineered to have a targeted disruption of the ASAP2 gene, enabling researchers to investigate the gene’s biological role in various cellular processes such as cell migration, adhesion, and signaling pathways.
The key function of the ASAP2 knockout cell lines lies in their ability to serve as a powerful tool for studying the consequences of absent ASAP2 expression. By leveraging techniques such as CRISPR-Cas9, these cell lines provide a reliable platform for examining changes in cellular behavior and molecular signaling, providing insights into the gene's contribution to diseases such as cancer and neurodegeneration. Researchers can easily compare the phenotypic and genotypic traits of these knockout lines against their wild-type counterparts, facilitating detailed explorations of the underlying mechanisms of ASAP2 in health and disease.
The scientific importance of ASAP2 Gene Knockout Cell Lines is underscored by their diverse applications in both fundamental and translational research. They can be instrumental in drug discovery, genetic screening, and functional genomics, making them valuable assets in the pursuit of novel therapeutic strategies. Furthermore, their use can aid in validating drug targets and understanding off-target effects, ultimately enhancing the reliability of preclinical studies.
Compared to traditional gene knockout methods, these cell lines offer specific advantages, such as validated knockout efficiency and consistent performance across experiments. Researchers benefit from reduced variability and enhanced reproducibility, allowing for more accurate data interpretation.
In a rapidly evolving field where precision and rigor are paramount, the ASAP2 Gene Knockout Cell Lines stand out as an invaluable resource for both academic and clinical laboratories. Investing in these specialized models empowers scientists to unlock novel biological insights and drive meaningful advancements in research. At our company, we pride ourselves on leading the way in providing innovative biological products backed by rigorous research and development, ensuring our customers have access to cutting-edge resources that support their scientific endeavors.
Please note that all services are for research use only. Not intended for any clinical use.
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