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APOBEC3A_B Knockout Cell Lines

Gene: APOBEC3A_B

Official Full Name: APOBEC3A and APOBEC3B deletion hybridprovided by HGNC

Gene Summary: This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. The protein encoded by this gene is the same as that encoded by APOBEC3A; however, this gene is a hybrid gene that results from the deletion of approximately 29.5 kb of sequence between the APOBEC3A gene and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes, so the deletion hybrid is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. [provided by RefSeq, Jul 2012]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO20989 APOBEC3A_B Knockout cell line (HeLa) Human APOBEC3A_B 1:3~1:6 Negative Online Inquiry
KO20990 APOBEC3A_B Knockout cell line (HCT 116) Human APOBEC3A_B 1:2~1:4 Negative Online Inquiry
KO20991 APOBEC3A_B Knockout cell line (HEK293) Human APOBEC3A_B 1:3~1:6 Negative Online Inquiry
KO20992 APOBEC3A_B Knockout cell line (A549) Human APOBEC3A_B 1:3~1:4 Negative Online Inquiry

Background

APOBEC3A_B Gene Knockout Cell Lines are specialized cell lines in which the APOBEC3A_B gene has been entirely disrupted through targeted gene-editing techniques. These cell lines are invaluable for studying the biological roles and mechanisms associated with APOBEC3A_B, a protein implicated in various biological processes, including DNA editing, immune response, and the modulation of viral replication. By using CRISPR-Cas9 or similar technologies to create knockout variants, researchers can delve into the functional aspects of this gene and its impact on cellular behavior.

The knockout mechanism allows scientists to observe phenotypic changes, gene expression levels, and biochemical pathways that are influenced by the absence of APOBEC3A_B. This understanding is critical in both fundamental research as well as in applied sciences, where elucidating the gene's role in oncogenesis, autoimmune diseases, and viral infections can lead to novel therapeutic strategies. The use of these cell lines in high-throughput screening assays enhances their utility in drug discovery and development, making them a cornerstone for translational research and clinical applications.

Compared to other gene knockout models, our APOBEC3A_B Gene Knockout Cell Lines offer several advantages. They are produced in an established, stable cellular background, ensuring reproducibility and consistency in experimental outcomes. Moreover, extensive validation processes guarantee that the knockout is both complete and specific, significantly reducing the risk of off-target effects that can confound experimental results.

For researchers and clinicians, these cell lines represent a powerful toolset for investigating the multifaceted roles of APOBEC3A_B in health and disease. By employing our product, users gain access to high-quality, well-characterized genomic resources that streamline their research endeavors and increase the likelihood of impactful discoveries.

Our company, with its deep expertise in genetic engineering and a commitment to advancing scientific research, stands at the forefront of providing innovative biological products that cater to the evolving needs of laboratory investigations. We are dedicated to supporting the scientific community with robust solutions that empower researchers to achieve their objectives effectively and efficiently.

Please note that all services are for research use only. Not intended for any clinical use.

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