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AKTIP Knockout Cell Lines

Gene: AKTIP

Official Full Name: AKT interacting proteinprovided by HGNC

Gene Summary: The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO19903 AKTIP Knockout cell line (HeLa) Human AKTIP 1:3~1:6 Negative Online Inquiry
KO19904 AKTIP Knockout cell line (HCT 116) Human AKTIP 1:2~1:4 Negative Online Inquiry
KO19905 AKTIP Knockout cell line (HEK293) Human AKTIP 1:3~1:6 Negative Online Inquiry
KO19906 AKTIP Knockout cell line (A549) Human AKTIP 1:3~1:4 Negative Online Inquiry

Background

AKTIP Gene Knockout Cell Lines are highly specialized cellular models engineered to facilitate targeted gene editing applications through the use of CRISPR/Cas9 technology. Each cell line features a specific gene of interest rendered non-functional, enabling researchers to investigate loss-of-function phenotypes, gain insights into gene function, and elucidate biological pathways. This precise gene knockout capability allows scientists to dissect complex biological processes, study disease mechanisms, and explore therapeutic targets with unprecedented specificity and accuracy.

The key mechanism behind AKTIP Gene Knockout Cell Lines revolves around the CRISPR/Cas9 system, which provides a robust method for genomic editing. By employing guide RNA (gRNA) that directs the Cas9 nuclease to specific genomic loci, researchers can induce double-strand breaks, leading to repairs through non-homologous end joining (NHEJ) that often results in the loss of gene function. This innovative methodology ensures a high fidelity of gene disruption, making these cell lines indispensable tools in functional genomics and molecular biology.

In research and clinical applications, AKTIP Gene Knockout Cell Lines serve as critical assets for advancing cancer studies, drug discovery, and regenerative medicine. Their ability to mimic disease states accurately aids in the identification of candidate therapeutics and enables high-throughput screening, thereby accelerating the drug development process. Furthermore, these cell lines are essential for gene therapy research, where understanding gene function is crucial for designing effective interventions.

What sets AKTIP Gene Knockout Cell Lines apart from traditional knockout models is the speed and efficiency of their generation, combined with their high reproducibility and specificity. Compared to older methods such as homologous recombination, the CRISPR/Cas9-based approach used in creating these cell lines significantly reduces the time required for gene alteration, permitting researchers to achieve results rapidly and reliably.

In summary, AKTIP Gene Knockout Cell Lines present researchers and clinicians with a powerful tool for dissecting gene functions and advancing biological discovery. The versatility and precision of these products enhance the ability to explore the complexities of genetic regulation and disease pathogenesis. Our company is dedicated to providing researchers with top-tier biological tools, backed by extensive expertise in genomics and gene editing technologies, ensuring that our products meet the highest scientific standards and contribute meaningfully to advancing life sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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