Gene: AKT1
Official Full Name: AKT serine/threonine kinase 1provided by HGNC
Gene Summary: This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
GP00266 | AKT1 gRNA1-gRNA2 KO plasmid | AKT1 | $850 | |||
KO00685 | AKT1 Knockout cell line(A549) | Human | AKT1 | 1:3~1:4 | Negative | Online Inquiry |
KO01354 | AKT1 Knockout cell line (HeLa) | Human | AKT1 | 1:3~1:6 | Negative | Online Inquiry |
KO11336 | AKT1 Knockout cell line (HCT 116) | Human | AKT1 | 1:2~1:4 | Negative | Online Inquiry |
KO11337 | AKT1 Knockout cell line (HEK293) | Human | AKT1 | 1:3~1:6 | Negative | Online Inquiry |
AKT1 Gene Knockout Cell Lines are a powerful tool in molecular biology and biomedical research, designed specifically to elucidate the roles of the AKT1 gene, a key player in the regulation of cellular processes such as metabolism, growth, and apoptosis. These cell lines are genetically engineered to have a complete deletion of the AKT1 gene, facilitating the study of AKT signaling pathways and their implications in various diseases, including cancer, diabetes, and cardiovascular disorders.
The primary function of AKT1 Gene Knockout Cell Lines lies in their ability to provide a model for studying the downstream effects of AKT signaling. By removing the AKT1 gene, researchers can evaluate changes in cellular behavior, such as proliferation rates, survival mechanisms, and response to therapeutic agents, thereby uncovering the molecular underpinnings of AKT-related pathologies. This assists in identifying potential targets for drug development and understanding resistance mechanisms in cancer therapy.
Scientifically, these cell lines serve as a critical resource in both research and clinical applications. They enable screening for new anti-cancer compounds, the investigation of metabolic disorders, and the exploration of AKT1’s role in cell differentiation and tissue regeneration. By generating insights into cellular signaling pathways, these models aid in translating basic research findings into clinical therapies.
One of the distinct advantages of our AKT1 Gene Knockout Cell Lines compared to alternative models, such as wild-type lines, is the ability to directly observe the absence of AKT1-mediated signaling. This direct observation minimizes confounding variables typically associated with knockdown approaches using RNA interference, thus providing clearer, more reliable data. Additionally, our cell lines are rigorously characterized and validated, assuring researchers of their authenticity and reproducibility.
For researchers and clinicians, leveraging AKT1 Gene Knockout Cell Lines opens the doors to deeper investigations into therapeutics targeting the AKT pathway. The ability to model human-like cellular responses fosters greater relevance to human diseases, ultimately driving innovation in treatment development.
Our company prides itself on its extensive experience in creating high-quality biological products that meet the evolving needs of the scientific community. With a commitment to excellence and innovation, we stand ready to support your research endeavors with our advanced AKT1 Gene Knockout Cell Lines and other specialized offerings.
Please note that all services are for research use only. Not intended for any clinical use.
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