Synthetic biology is a rapidly evolving field that has revolutionized the way we study and manipulate living systems. One of the key techniques used in synthetic biology is inducible gene expression, which allows researchers to control gene expression in response to external stimuli. Inducible gene expression control is particularly useful in the production of recombinant proteins, where it is often desirable to induce expression of the target gene only when needed, reducing the metabolic burden on the host cell.
Figure 1: Early steps in the transcription cycle. (Weake, V., Workman, J. 2010)
CD Biosynsis offers a range of services for inducible gene expression control in strains. Here we do our best to help you for your inducible gene expression control needs.
At CD Biosynsis, we offer a comprehensive range of services for inducible gene expression control. Our services are designed to provide flexible and customizable solutions to meet the specific needs of our clients. Below are some of the specific services that we offer:
Our team of experts can design and construct inducible expression vectors for a variety of applications. We use a range of inducible promoters, such as the Tet-On and Tet-Off systems, to control gene expression in response to specific stimuli. Our vector design process involves:
We offer cell line generation services to introduce the inducible expression vector into the host cell line. Our team of experts can engineer a wide range of cell lines, including HEK293, CHO, and NIH3T3. Our cell line generation process involves:
We understand that the optimization of expression conditions is crucial to achieving high-level expression of the target gene. We can optimize a range of expression conditions, including inducer concentration, time course, and cell culture conditions, to ensure optimal expression of the target gene in the host cell line. Our optimization process involves:
We offer gene expression analysis services to monitor gene expression in the host cell line. Our team of experts can use a range of analytical techniques, including qPCR and western blotting, to quantify gene expression levels in the host cell line. Our gene expression analysis process involves:
At CD Biosynsis, we can provide scale-up and production services for inducible gene expression. Our scale-up and production services are designed to meet the specific needs of our clients and can be tailored to their specific requirements. Our scale-up and production process involves:
At CD Biosynsis, we offer a range of services for inducible gene expression control in strains, including the construction of inducible expression vectors, strain engineering, and optimization of expression conditions. Contact us today to learn more about our services and how we can help you achieve your research goals.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.