Introduction
Synthetic biology is a rapidly evolving field that has revolutionized the way we study and manipulate living systems. One of the key techniques used in synthetic biology is inducible gene expression, which allows researchers to control gene expression in response to external stimuli. Inducible gene expression control is particularly useful in the production of recombinant proteins, where it is often desirable to induce expression of the target gene only when needed, reducing the metabolic burden on the host cell.
Figure 1: Early steps in the transcription cycle. (Weake, V., Workman, J. 2010)
CD Biosynsis offers a range of services for inducible gene expression control in strains. Here we do our best to help you for your inducible gene expression control needs.
Our Services for Inducible Gene Expression Control
At CD Biosynsis, we offer a comprehensive range of services for inducible gene expression control. Our services are designed to provide flexible and customizable solutions to meet the specific needs of our clients. Below are some of the specific services that we offer:
Vector Design and Construction
Our team of experts can design and construct inducible expression vectors for a variety of applications. We use a range of inducible promoters, such as the Tet-On and Tet-Off systems, to control gene expression in response to specific stimuli. Our vector design process involves:
- Consultation with the client to understand their specific needs and requirements.
- Design of the inducible expression vector using state-of-the-art software tools.
- Construction of the vector using standard molecular biology techniques.
Cell Line Generation
We offer cell line generation services to introduce the inducible expression vector into the host cell line. Our team of experts can engineer a wide range of cell lines, including HEK293, CHO, and NIH3T3. Our cell line generation process involves:
- Selection of the appropriate host cell line based on the client's specific needs and requirements.
- Introduction of the inducible expression vector into the host cell line using a range of transfection techniques.
- Verification of the successful integration of the inducible expression vector into the host cell line.
Optimization of Expression Conditions
We understand that the optimization of expression conditions is crucial to achieving high-level expression of the target gene. We can optimize a range of expression conditions, including inducer concentration, time course, and cell culture conditions, to ensure optimal expression of the target gene in the host cell line. Our optimization process involves:
- Determination of the optimal inducer concentration by conducting a series of inducer titration experiments.
- Optimization of the time course for induction to maximize gene expression.
- Monitoring of gene expression using a range of analytical techniques, including qPCR and western blotting.
Gene Expression Analysis
We offer gene expression analysis services to monitor gene expression in the host cell line. Our team of experts can use a range of analytical techniques, including qPCR and western blotting, to quantify gene expression levels in the host cell line. Our gene expression analysis process involves:
- Sample collection and preparation.
- RNA isolation and quantification.
- Reverse transcription and qPCR analysis or protein extraction and western blotting analysis.
- Data analysis and interpretation.
Scale-Up and Production
At CD Biosynsis, we can provide scale-up and production services for inducible gene expression. Our scale-up and production services are designed to meet the specific needs of our clients and can be tailored to their specific requirements. Our scale-up and production process involves:
- Optimization of the production process to maximize gene expression.
- Scale-up of the production process to meet the specific needs of the client.
- Purification of the recombinant protein using standard chromatography techniques.
Our Advantages
- Customized solutions tailored to specific needs.
- Fast turnaround times for efficient research.
- High-quality results with state-of-the-art equipment.
- Competitive pricing without sacrificing quality.
- Exceptional customer support throughout the research process.
At CD Biosynsis, we offer a range of services for inducible gene expression control in strains, including the construction of inducible expression vectors, strain engineering, and optimization of expression conditions. Contact us today to learn more about our services and how we can help you achieve your research goals.
References
- Weake, V., Workman, J. Inducible gene expression: diverse regulatory mechanisms. Nature Reviews Genetics 11, 426–437 (2010).
- Cuartero, S., et al. Control of inducible gene expression links cohesin to hematopoietic progenitor self-renewal and differentiation. Nature Immunology 19, 932–941 (2018).
- Hargreaves, D. C., et al. Control of inducible gene expression by signal-dependent transcriptional elongation. Cell, 138(1), 129–145 (2009).
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