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PRKAR1A Knockout Cell Lines

Gene: PRKAR1A

Official Full Name: protein kinase cAMP-dependent type I regulatory subunit alphaprovided by HGNC

Gene Summary: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed. [provided by RefSeq, Jan 2013]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO05025 PRKAR1A Knockout cell line (HeLa) Human PRKAR1A 1:3~1:6 Negative Online Inquiry
KO05026 PRKAR1A Knockout cell line (HCT 116) Human PRKAR1A 1:2~1:4 Negative Online Inquiry
KO05027 PRKAR1A Knockout cell line (HEK293) Human PRKAR1A 1:3~1:6 Negative Online Inquiry
KO05028 PRKAR1A Knockout cell line (A549) Human PRKAR1A 1:3~1:4 Negative Online Inquiry

Background

PRKAR1A Gene Knockout Cell Lines are genetically engineered cell models designed specifically to study the role of the PRKAR1A gene, a crucial component in the protein kinase A (PKA) signaling pathway. The precise knockout of this gene allows researchers to explore its involvement in various physiological and pathological processes, such as cell metabolism, growth regulation, and cancer development. The absence of PRKAR1A provides a unique opportunity to observe the downstream effects on signaling cascades and other cellular mechanisms, contributing to a deeper understanding of cellular physiology.

The key function of these knockout cell lines is to eliminate the expression of the PRKAR1A protein, facilitating the study of its regulatory role in the PKA pathway. This mechanism is vital to investigators aiming to dissect the molecular underpinnings of diseases associated with aberrant PKA signaling, including certain tumors and endocrine disorders. By utilizing these knockout cells, researchers can perform experiments such as drug screening, gene expression analysis, and signaling pathway characterization with greater accuracy.

The scientific importance of PRKAR1A Gene Knockout Cell Lines in both basic and applied research is underscored by their versatile applications in drug discovery and the development of therapeutic strategies. Their use in clinical settings can potentially lead to breakthroughs in understanding resistance mechanisms in cancer therapies and the identification of novel drug targets.

Compared to traditional cell lines, these knockout models offer specific advantages, such as enhanced reproducibility and more straightforward interpretation of results. They serve as a powerful tool for researchers seeking reliability and fidelity in their experiments, particularly when examining signaling pathways that have significant implications for human health.

Investing in PRKAR1A Gene Knockout Cell Lines means equipping your laboratory with innovative tools to advance your research projects and clinical applications. With our company's strong background in generating high-quality genetic models, we are committed to supporting the scientific community with reliable resources that facilitate groundbreaking discoveries in the field of genetics and cell biology.

Please note that all services are for research use only. Not intended for any clinical use.

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