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MBD5 Knockout Cell Lines

Gene: MBD5

Official Full Name: methyl-CpG binding domain protein 5provided by HGNC

Gene Summary: This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause an autosomal dominant type of cognitive disability. The encoded protein interacts with the polycomb repressive complex PR-DUB which catalyzes the deubiquitination of a lysine residue of histone 2A. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq, Jul 2017]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO16313 MBD5 Knockout cell line (HeLa) Human MBD5 1:3~1:6 Negative Online Inquiry
KO16314 MBD5 Knockout cell line (HCT 116) Human MBD5 1:2~1:4 Negative Online Inquiry
KO16315 MBD5 Knockout cell line (HEK293) Human MBD5 1:3~1:6 Negative Online Inquiry
KO16316 MBD5 Knockout cell line (A549) Human MBD5 1:3~1:4 Negative Online Inquiry

Background

MBD5 Gene Knockout Cell Lines represent a pivotal advancement in functional genomics, offering researchers the ability to study the biological implications of the MBD5 gene, which is associated with various neurodevelopmental disorders. These engineered cell lines are created through the precise deletion of the MBD5 gene, enabling the investigation of its role in cellular pathways, gene expression, and interactions within neural contexts.

Key functions of the MBD5 Gene Knockout Cell Lines include the ability to elucidate the mechanisms through which MBD5 influences chromatin structure and gene regulation. Researchers utilize these cell lines to assess changes in gene expression, assess neuronal differentiation, and explore the consequences of gene silencing on cellular behavior. By providing an experimental model that mimics the loss-of-function mutations found in patients, these cell lines serve as an invaluable tool for dissecting the pathophysiology underlying specific neurodevelopmental phenotypes.

In addition to their relevance in basic research, MBD5 Gene Knockout Cell Lines possess significant applications in drug discovery and personalized medicine. Understanding the molecular pathways altered by MBD5 loss can guide therapeutic target identification and the development of innovative treatment strategies for disorders such as autism spectrum disorders and intellectual disabilities.

Compared to alternatives such as RNA interference or pharmacological inhibition, knockout cell lines offer a more stable and permanent genetic edit, allowing for long-term studies and robust validations of experimental results. This stability enhances reproducibility and reliability across experiments, ensuring that researchers can confidently draw conclusions from their findings.

The value of MBD5 Gene Knockout Cell Lines extends beyond laboratory research; they empower scientists to generate insights that may translate into clinical applications, ultimately improving patient outcomes. Our company specializes in the development of high-quality biological models tailored for advanced research. By prioritizing precision and reliability, we position ourselves as a trusted partner in the scientific community, assisting researchers in making groundbreaking discoveries.

Please note that all services are for research use only. Not intended for any clinical use.

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