PTEN Knockout cell line (TPC-1)

Catalog Number: KOA60318

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Specifications

Product Information
Product Name	PTEN Knockout cell line (TPC-1)
specification	1*10^6
Storage and transportation	Shipped on dry ice; Store in liquid nitrogen
Cell morphology	Epithelial-like, adherent
Passage ratio	1:5-1:8
species	Human
Gene	PTEN
Gene ID	5728
Build method	Electroporation/Lentivirus
Mycoplasma testing	negative
Cultivation system	90%DMEM+10%FBS
Parental Cell Line	TPC-1
Quality Control	Genotype: PTEN Knockout cell line (TPC-1)>95% viability before freezing. All cells were tested and found to be free of bacterial, viruses,mycoplasma and other toxins.

Gene Information
Gene Official Full Name	phosphatase and tensin homologprovided by HGNC
Also known as	BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; PTENgama
Gene Description	This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]
Expression	Ubiquitous expression in fat (RPKM 42.8), spleen (RPKM 28.6) and 25 other tissues See more

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