CD Biosynsis offers a comprehensive suite of Metabolic Engineering Method Services, providing researchers and industry partners with precise tools for optimizing biological systems. Our services cover the full spectrum of synthetic biology techniques, ranging from rational Pathway Engineering for enhancing biochemical production to complex Genetic Circuit Design for precise cellular control. By leveraging these cutting-edge methodologies—including gene manipulation, regulatory element design, and genomic integration—we enable the development of highly efficient, stable, and controllable microbial and cellular factories for advanced biomanufacturing and fundamental research.
Get a QuoteModern metabolic engineering relies on a diverse toolkit of genetic and synthetic biology methods. These methods are broadly categorized into strategies for direct metabolic pathway modification (Pathway Engineering) and the introduction of novel regulatory control systems (Genetic Circuit Engineering). Effective application of these methods requires advanced DNA synthesis, high-throughput screening, and computational design to ensure the stability, efficiency, and precise control of the engineered biological system.
Rational design and construction of novel or optimized biosynthetic pathways for target compound production.
Eliminate competing native pathways or degradation routes to redirect metabolic flux towards the desired product.
Boost the expression of rate-limiting enzymes to overcome pathway bottlenecks and maximize product formation.
Fine-tuning gene expression levels to achieve optimal pathway balance and minimize cellular burden.
Engineering the host cell environment to improve stress tolerance, enhance precursor supply, and increase overall viability.
Precise, site-specific insertion of entire metabolic pathways into the host genome for stable and reliable expression.
Efficient method for integrating large genetic constructs, often used for screening optimal genomic integration sites.
Design and construction of synthetic genetic circuits (switches, oscillators, logic gates) to control metabolism or cell fate.
Creation of novel regulatory elements to achieve tightly controlled, tunable, or inducible gene expression.
Engineering distal regulatory elements to significantly boost or fine-tune the activity of existing promoters.
Assembly of multiple genetic parts into functional, complex regulatory networks (circuits).
Rigorous characterization using flow cytometry and time-lapse imaging to confirm predicted circuit behavior and function.
Our workflow is structured to facilitate rational design and precise implementation of both metabolic pathways and regulatory circuits.
Computational Design & Modeling
Genetic Part Construction
System Integration & Screening
Functional Testing & Validation
Utilize computational tools (FBA, circuit simulation) to design optimal pathways and predict circuit behavior.
Identify target genes, regulatory elements, and required gRNAs for modification or construction.
Define expression levels needed for pathway balance using promoter libraries.
High-fidelity synthesis of long DNA sequences, promoters, and enhancers.
Assembly of genetic parts into functional operons or complex circuits using automated platforms.
Construction of CRISPR vectors or transposon delivery systems.
Rigorously characterize pathway performance (titer, yield, purity) using advanced analytical methods (LC-MS/MS).
Validate genetic circuit function (e.g., induction ratio, switching behavior) using flow cytometry and microscopy.
Delivery of the final engineered system and a complete data package.
Expertise in CRISPR & Transposon Systems
Utilize the most advanced tools for stable genomic integration of large, multi-gene pathways, ensuring long-term strain stability.
Precision Regulatory Control
Specialized services in Synthetic Promoter and Enhancer Design for finely tuning gene expression and creating inducible systems.
DBTL Cycle for Circuit Validation
We apply the Design-Build-Test-Learn framework to circuit engineering, rigorously testing and validating complex control logic.
Holistic System Optimization
Integrated approach that addresses both the metabolic pathway and the host chassis, ensuring maximum system performance.
"The combination of CRISPR Integration and Promoter Engineering was revolutionary for our project. CD Biosynsis not only introduced a complex pathway but also provided the precise control needed to balance the intermediate flux, eliminating cell toxicity issues."
Dr. Liam O'Connell, Lead Synthetic Biologist
"We used their Genetic Circuit Engineering service to build a molecular switch that only activates product synthesis under specific environmental stress. The validation data perfectly matched the computational design, a testament to their technical accuracy."
Dr. Mei Lin, Research Scientist, BioTech Startup
"Their Chassis Optimization greatly improved our strain's stress tolerance. This foundational work, combined with pathway tuning, allowed us to achieve high-density fermentation that was previously impossible."
Mr. Alex Johnson, Fermentation Process Manager
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look toward further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the primary benefit of using Genetic Circuits in metabolic engineering?
Genetic circuits provide temporal and spatial control over gene expression. This allows engineers to switch pathways on/off at optimal fermentation stages, regulate toxic intermediate levels, or implement feedback loops to stabilize production, significantly boosting yield and robustness.
How do Promoter and Enhancer engineering services differ?
The Promoter is the direct starting point for transcription and controls the basal and maximal expression rate of a gene. The Enhancer is a distal regulatory element that can significantly amplify (or fine-tune) the promoter's activity, often providing an extra layer of control and strength that native promoters lack.
Why is Genomic Integration preferred over plasmids for pathway engineering?
Genomic integration (via CRISPR or Transposon) ensures the permanent and stable inheritance of the engineered pathway across generations, especially under non-selective large-scale fermentation conditions. Plasmids can be lost or their copy numbers can fluctuate, leading to unstable production.
What kind of Validation is done for genetic circuits?
Circuit validation involves quantifying the circuit's response to inputs (e.g., inducers) over time. This is done through tools like Flow Cytometry (measuring fluorescence of reporter proteins across the population) and Time-Lapse Microscopy (observing single-cell behavior) to ensure the circuit behaves as predicted by the model.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.