CD Biosynsis offers comprehensive E. coli Genome Editing & Metabolic Engineering Solutions , providing an integrated platform to design, build, test, and optimize microbial hosts for the production of target compounds, protein therapeutics, and novel chemicals. Our services span from precise genomic modification to advanced pathway construction and high-throughput assay development . We utilize state-of-the-art technologies including CRISPR/Cas9 and computational modeling to rapidly transform E. coli into highly efficient biological factories, accelerating research and commercial bioprocesses.
Get a QuoteAs the premier host organism in biotechnology, E. coli offers unparalleled speed, scalability, and genetic tractability. Our integrated solutions platform is designed to provide end-to-end support for your project: starting with the fundamental genome engineering of the chassis, progressing through the metabolic optimization for high-yield bioproduction, and concluding with protein expression and quantitative assay testing . By combining these core capabilities, we deliver optimized strains and bioprocesses efficiently, reducing time and cost in the development cycle.
Core Capabilities
Utilizing powerful tools like CRISPR/Cas9 and Red/ET Recombineering for accurate genome modifications, including gene knockouts, point mutations, and targeted gene insertions to build optimized host strains.
Key Applications
Creating custom strains for enhanced pathway integration, increasing product tolerance, and removing competitive metabolic pathways to boost target production yield.
Core Capabilities
Applying the Design-Build-Test-Learn (DBTL) cycle to construct complex, novel biosynthetic pathways, optimize enzyme expression, and implement dynamic genetic control systems for pathway regulation.
Key Applications
Accelerating the development of sustainable biomanufacturing processes for high-value compounds such as fuels, APIs, flavors, and specialty chemicals.
Core Capabilities
End-to-end service from gene optimization to multi-step chromatographic purification (Affinity, IEX, SEC), delivering proteins with guaranteed high purity (up to >98%) and low endotoxin levels.
Key Applications
Producing functional proteins for structural biology, biophysics studies, therapeutic drug development, and target-based in vitro assay development.
Core Capabilities
Integration of Flux Balance Analysis (FBA) and Genome-Scale Metabolic Models (GSMMs) with the development of E. coli biosensors for high-throughput pathway screening.
Key Applications
Predicting optimal genetic modifications, identifying metabolic bottlenecks, and enabling rapid, quantitative testing of pathway variants for superior yield.
Core Capabilities
Offering robust assay development (Z'-factor > 0.5) for both purified targets ( in vitro enzyme kinetics, PPIs) and whole-cell assays ( in vivo reporter systems, MIC/EC50 testing).
Key Applications
Validating the efficacy of drug candidates, measuring compound toxicity, and quantifying the performance of engineered genetic circuits or metabolic modules in a cellular context.
A systematic process connecting genome-level changes to product optimization.
Design & Engineering
Build & Optimization
Test & Quantification
Scale-Up & Production
Computational: Apply FBA and GSMMs to rationally design the host genome and target pathway for optimal performance.
Strategy: Define target modifications (knockouts/insertions) and select appropriate gene expression and purification strategies.
Genome Editing: Execute precise modifications using CRISPR/Cas9 or Recombineering to build the optimal E. coli chassis.
Pathway Assembly: Clone and assemble multi-gene synthetic pathways and optimize expression for balanced flux.
Assay Screening: Validate strain and pathway performance using high-throughput in vitro and in vivo assays, quantifying product titer and purity.
Iteration: Use quantitative data to refine the computational model and guide the next round of engineering for yield improvement.
Integrated DBTL Platform
Seamless connection between computational design, genome editing, and quantitative validation for fastest time-to-market.
Cutting-Edge Genome Tools
Expert application of CRISPR/Cas9, Red/ET, and MAGE for precise, high-efficiency, and multiplexed genetic modifications.
Quality & Purity Assurance
Guaranteed protein purity (>98%) and rigorous assay validation (Z'-factor) ensure reliable data and high-quality final products.
Comprehensive Expertise
End-to-end support for metabolic engineering, protein expression, and drug screening, all centralized on the E. coli platform.
"The integrated approach, combining FBA modeling with CRISPR-based genome editing, was key. It led to a 10-fold increase in our target compound's titer within six months, a timeline we previously thought impossible."
Dr. Sarah Chen, Head of Bioprocess Optimization
"CD Biosynsis successfully purified a highly toxic membrane protein from E. coli with over 98% purity and low endotoxin. Their expertise in solubility optimization was crucial for our structural studies."
Dr. Alex Johnson, Principal Investigator, Structural Biology
"Their custom-developed whole-cell assay provided the fast, reliable EC50 data we needed to validate our lead drug candidate's cellular efficacy before moving to mammalian models."
Ms. Nicole Kim, Research Scientist, Pharmaceutical Discovery
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
Still have questions?
Contact UsWhat is the key advantage of an integrated E. coli platform?
The integrated platform ensures seamless transition between the different phases of strain development: from predictive modeling and precise genome modification to high-throughput validation and final protein or chemical production, significantly reducing project lead time and minimizing bottlenecks.
What is the typical starting material required for a metabolic engineering project?
We typically require the full sequence of the target biosynthetic pathway genes. If you don't have this, we can start with gene synthesis and optimization. We will handle the host strain selection and engineering using our proprietary and standard E. coli chassis.
Do you offer customized solutions for toxic or insoluble proteins?
Yes. Our protein expression services specialize in challenging targets. We employ solubility tags, chaperone co-expression, specialized low-temperature induction, and inclusion body refolding techniques to maximize the yield of functional, soluble protein.
Can I combine Genome Editing with Assay & Modeling services?
Absolutely. This combination is highly recommended. Our computational modeling (FBA) predicts the best gene editing targets, and our assays (In Vivo/In Vitro) provides the precise data needed to validate and further refine the engineered strains in an iterative DBTL cycle.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.