CD Biosynsis offers expert, end-to-end Protein Expression and Purification Services for the production of high-quality recombinant proteins across various expression systems. Our service is designed to support diverse research and commercial needs, from small-scale analytical quantities to large-scale production runs. We manage the entire workflow, starting with Codon Optimization for enhanced expression, followed by selecting the optimal host system (E. coli, Yeast, Insect, Mammalian) to ensure correct folding and post-translational modifications, and concluding with high-purity purification and rigorous quality control. We specialize in challenging targets, including membrane proteins and complex fusion proteins, guaranteeing the delivery of functionally active protein for your downstream applications.
Get a QuoteHigh-quality, correctly folded, and functionally active recombinant proteins are the foundation of drug discovery, structural biology, and diagnostics. Suboptimal expression and purification can lead to low yield, misfolding (inclusion bodies), or incorrect post-translational modifications (PTMs), compromising downstream results. Our service addresses these challenges using a systematic approach that optimizes the gene sequence, matches the protein to the most appropriate expression host, and employs tailored chromatography techniques to ensure high purity and integrity.
Algorithm-driven design to enhance translation efficiency and yield in the target host organism.
Optimized for various bacterial hosts to maximize soluble protein expression.
Tailored codon usage to prevent rare codon stalling in E. coli expression systems.
Improving gene expression efficiency for Pichia and S. cerevisiae platforms.
Enhancing gene expression in CHO and HEK cells for high-yield therapeutic protein production.
Co-expression strategies for protein complexes or pathways requiring multiple components.
High-speed, cost-effective production in E. coli and Bacillus subtilis for non-glycosylated proteins.
Standard service utilizing E. coli as the industry-leading system, including refolding protocols.
Specialized service for secreting soluble proteins, reducing purification complexity.
Ideal for moderate complexity proteins requiring simple PTMs and large-scale, cost-effective fermentation.
High-density fermentation system known for high yield and secreted protein production.
Excellent for complex, oligomeric, and heavily PTM-modified proteins using the Baculovirus system.
Gold standard for therapeutic proteins and complex PTMs, providing the most native folding.
Development of stable CHO cell lines for GMP-compliant therapeutic protein manufacturing.
Specialized protocols for solubilization and stabilization of GPCRs and ion channels.
Design, expression, and purification of fusion proteins, including therapeutic Fc-fusions.
Targeted expression in systems that ensure correct glycosylation patterns for functional activity.
Our systematic workflow ensures delivery of high-quality, functionally active recombinant proteins.
Gene Synthesis & Optimization
Expression Vector Construction
Protein Expression & Screening
Purification & QC
Customer provides AA sequence or gene sequence.
Codon Optimization: Design the gene sequence for maximal expression and solubility in the selected host.
Final gene synthesis and QC verification.
Subcloning of the optimized gene into the appropriate expression vector (e.g., pET, Baculovirus, pCMV).
Inclusion of suitable tags (His-tag, GST) and cleavage sites.
Host cell transformation or transfection (transient or stable).
Purification: Multi-step chromatography (e.g., Ni-NTA, SEC, Ion Exchange) tailored to the protein.
Quality Control: SDS-PAGE, Western Blot, HPLC, Mass Spectrometry to confirm purity, integrity, and mass.
Final delivery of the protein at the required concentration and purity level.
Integrated Optimization Pipeline
Our service links Codon Optimization directly to the expression system, guaranteeing maximum expression efficiency from the start.
Multi-System Expertise
Proficiency in all major expression platforms (E. coli, Yeast, Baculovirus, Mammalian) to ensure the perfect match for any protein complexity.
Specialized for Challenging Proteins
Proven track record in successfully producing difficult targets like functional Membrane Proteins and Glycoproteins.
Rigorous Quality Control
We guarantee high purity (>95\% or higher) and provide detailed QC data (SDS-PAGE, MS) to confirm protein identity and integrity.
"The Mammalian Codon Optimization service was instrumental in increasing the yield of our therapeutic antibody in CHO cells by 70\%. The resulting protein was of exceptional purity and activity."
Dr. Emily Clarkson, VP of Biologics Development
"We had a challenging GPCR that would not express in E. coli. CD Biosynsis successfully produced the functional Membrane Protein using their Baculovirus system, enabling our structural studies."
Prof. Kenji Tanaka, Director of Structural Biology
"Their Fusion Protein Production service quickly delivered a high-quality Fc-fusion protein. The purification strategy was highly efficient, saving us significant time and cost in our assay development."
Ms. Anya Sharma, Lead Scientist, Diagnostic R\&D
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
Why is Codon Optimization necessary?
Codon optimization ensures that the gene sequence contains codons that are frequently used by the transfer RNAs of the host cell. This prevents ribosome stalling, increases translation speed, and dramatically boosts the overall yield of soluble, correctly folded protein.
Which expression system should I choose for my protein?
The choice depends on the protein complexity: E. coli for simple, non-glycosylated proteins; Yeast (Pichia) for high yield, simple PTMs; Insect Cells for complex folding and high molecular weight proteins; and Mammalian Cells (CHO/HEK) for therapeutic proteins requiring human-like PTMs and complex folding.
How do you handle proteins that express as Inclusion Bodies?
If E. coli expression results in inclusion bodies, we first try expression condition optimization (lower temperature, co-expression of chaperones). If unsuccessful, we use our specialized refolding protocols to solubilize and correctly refold the protein, followed by targeted purification.
What is the typical purity level you guarantee?
We typically guarantee a purity level of >90\% or >95\% for most standard proteins. We can achieve >98\% or even >99\% purity on request for demanding applications like structural studies or therapeutic development, using multi-step FPLC purification.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.