3-Hydroxypropionic Acid 3-HPA Engineering Service

3-Hydroxypropionic Acid (3-HPA) is recognized as a key Bio-monomer (Acrylic Acid Precursor) for the production of commercially important derivatives, notably acrylic acid, biodegradable polymers, and 1,3-propanediol. Its production is challenged by dual issues: Chemical conversion is costly; biological yield is low, and the product is unstable. The instability of 3-HPA leads to degradation during fermentation and recovery, limiting final productivity.

CD Biosynsis focuses on stabilizing the biological route for 3-HPA: Metabolic Engineering: Introduce the Glycerol Dehydratase pathway (Glycerol to 3-HPA) into E. coli or yeast. This route efficiently converts cheap, renewable glycerol. Crucially, we address the key bottlenecks by Cofactor Balance: Optimize coenzyme B12 regeneration and NADPH supply. This ensures the continuous, high-efficiency functioning of the Glycerol Dehydratase enzyme, leading to higher, more stable 3-HPA yields.

Pain Points

The production of 3-HPA for industrial applications faces these critical biological and chemical challenges:

• Product Instability: 3-HPA is highly reactive and prone to dehydration to Acrylic Acid or polymerization at fermentation temperatures, reducing the final recoverable yield.
• Cofactor Dependency: The Glycerol Dehydratase pathway relies on Coenzyme B12, which is expensive and irreversibly inactivated during the reaction, requiring costly regeneration systems.
• Low Titer and Selectivity: Incomplete pathway optimization and competing side reactions e.g. 1, 3-PDO formation limit the final titer and purity in current bio production systems.
• High Chemical Synthesis Cost: Alternative chemical routes e.g. from Ethylene Oxide involve harsh reagents and multiple steps, making them economically uncompetitive against fossil-fuel products.

Overcoming these stability and cofactor issues is key to making bio-based 3-HPA viable.

Solutions

CD Biosynsis utilizes Metabolic Engineering to stabilize the 3-HPA platform:

Glycerol Dehydratase Pathway Introduction

           

We introduce and optimize the Glycerol Dehydratase pathway Glycerol to 3-HPA in E. coli or yeast, using cheap Glycerol as the feedstock.

Coenzyme B12 Regeneration Optimization

We co-express B12 regeneration enzymes or develop non-B12 dependent pathways to eliminate the high cost and instability associated with B12.

Enhanced NADPH Supply

We metabolically engineer the host to overproduce NADPH e.g. via G6PDH overexpression, providing the necessary reducing power for high flux conversion.

Product Stability and Recovery Optimization

We design low-temperature fermentation protocols and in situ removal systems to prevent 3-HPA dehydration or polymerization in the broth.

Our solution ensures a sustainable, B12-independent, and stable supply of 3-HPA for bio-based Acrylic Acid production.

Advantages

Our 3-Hydroxypropionic Acid 3-HPA engineering service offers these core benefits:

Stable, High-Yield Production

Process and strain optimization mitigate product instability and degradation, maximizing recoverable 3-HPA titer.

Reduced Cofactor Cost

B12-independent pathways or efficient regeneration removes the major cost bottleneck of Glycerol Dehydratase systems.

Renewable Glycerol Feedstock

Utilizing crude glycerol a byproduct of biodiesel production ensures a cheap and abundant renewable carbon source.

Sustainable Acrylic Acid Precursor

3-HPA is a direct intermediate to bio-based Acrylic Acid, replacing petrochemical Propylene in superabsorbent polymers.

High Specificity Pathway

Engineered strains exhibit minimal byproduct formation e.g. 1, 3-PDO, leading to higher purity and simplified downstream processing.

We unlock the potential of 3-HPA as a sustainable building block for the chemical industry.

Process

Our 3-Hydroxypropionic Acid 3-HPA engineering service follows a rigorous, multi-stage research workflow:

• Glycerol Pathway Construction: Clone and optimize Glycerol Dehydratase and Aldehyde Dehydrogenase genes for efficient Glycerol to 3-HPA conversion in the chosen host.
• Cofactor Balance Optimization: Metabolically engineer the host for high-efficiency NADPH and B12 regeneration to sustain high Glycerol Dehydratase activity or design a B12-independent route.
• Stability and Product Removal: Develop low-temperature fermentation protocols and coupled in situ extraction systems to remove 3-HPA before it degrades.
• Feedstock Tolerance Optimization: Engineer strains to tolerate crude Glycerol impurities and high product concentrations for industrial scale-up.
• Product Purity Validation: Analyze final 3-HPA purity and stability over time via HPLC and validate minimal Acrylic Acid byproduct formation.

Technical communication is maintained throughout the process, focusing on timely feedback regarding yield and product stability attributes.

Explore the potential for a stable, high-yield 3-Hydroxypropionic Acid 3-HPA supply. CD Biosynsis provides customized strain and process engineering solutions:

• Detailed 3-HPA Titer, Yield, and Stability Reports g/L, percent theoretical, half-life in broth.
• Consultation on B12-independent pathway design to significantly reduce operational cost.
• Experimental reports include complete raw data on enzyme activities, NADPH and B12 levels, and 3-HPA degradation kinetics.