Phloroglucinol Engineering Service

Phloroglucinol is a valuable aromatic compound used as a Bio-monomers Resins/Dyes precursor for producing high-performance resins, UV absorbers, and pharmaceuticals. Its current supply chain faces significant limitations: Low yield and high cost of natural extraction from sources like algae or plants, and the alternative complex chemical synthesis route is inefficient. This necessitates a more sustainable and high-yield biomanufacturing method.

CD Biosynsis offers an advanced metabolic engineering platform for Phloroglucinol production: Metabolic Engineering: Engineer E. coli or yeast to biosynthesize Phloroglucinol from Malonyl-CoA and Acetyl-CoA via the Polyketide Synthase PKS pathway. This route is highly efficient, starting from cheap sugars. We optimize the biosynthetic cascade by focusing on: Overexpression of key PKS components and Cyclase enzymes. Maximizing the activity of the Polyketide Synthase and the downstream Cyclase is essential for high titer production and purity.

Pain Points

The supply chain for Phloroglucinol faces these key hurdles:

• Unsustainable Extraction: Natural extraction from plant or algae sources is season-dependent, involves complex purification steps, and results in very low yields, making the final product extremely expensive.
• Chemical Complexity: Chemical synthesis is multi-step, often involving harsh reagents and low overall yield, making it economically unattractive for large-scale production.
• PKS Pathway Bottlenecks: The biological route relies on the Polyketide Synthase PKS pathway, which is limited by the availability of precursor molecules like Malonyl-CoA and Acetyl-CoA.
• Enzyme Efficiency: The activity of the key terminal enzyme, the Cyclase, which forms the aromatic ring, often exhibits low efficiency and leads to the accumulation of linear intermediates.

A bio-based approach must ensure high precursor supply and efficient conversion by the PKS enzyme system.

Solutions

CD Biosynsis utilizes advanced metabolic and enzyme engineering to optimize Phloroglucinol production:

PKS Pathway Integration

           

We integrate the full PKS pathway e.g. TKS, PKS III into robust industrial hosts like E. coli or Y. lipolytica to convert simple carbon sources into Phloroglucinol.

PKS and Cyclase Overexpression

We overexpress the key PKS and Cyclase enzymes that form the final aromatic ring structure, utilizing strong, constitutive promoters to maximize their activity and overcome the rate-limiting steps.

Precursor Flux Enhancement

We engineer the central metabolism by enhancing the activity of Acetyl-CoA carboxylase and other upstream enzymes to ensure an ample and balanced supply of Malonyl-CoA and Acetyl-CoA precursors.

Byproduct Pathway Minimization

We use targeted gene deletion to eliminate competing metabolic pathways that divert carbon away from the PKS route, thereby maximizing the overall carbon yield for Phloroglucinol.

This approach establishes a high-yield, pure, and continuous supply chain for Phloroglucinol from simple sugars.

Advantages

Our Phloroglucinol engineering service is dedicated to pursuing the following production goals:

High Efficiency Aromatic Synthesis Icon

The PKS pathway offers a direct, high-yield route to the tri-hydroxylated aromatic structure of Phloroglucinol.

Avoid Complex Chemical Steps Icon

Bioproduction eliminates the need for the costly, low-yield multi-step chemical synthesis or natural extraction.

Maximize Precursor Availability Icon

Metabolic engineering ensures high availability of the Malonyl-CoA building block for the PKS route.

Robust Microbial Platform Icon

Engineered E. coli and yeast are scalable, fast-growing hosts ideal for industrial fermentation.

High Product Purity Icon

The specificity of the PKS enzyme cascade minimizes the formation of undesirable structural analogs.

We deliver a sustainable, high-titer cell factory for the production of Phloroglucinol and its derivatives.

Process

Our Phloroglucinol strain engineering service follows a rigorous, multi-stage research workflow:

• Precursor Supply Engineering: Overexpress the Acetyl-CoA carboxylase complex and related pathways to dramatically increase the intracellular pools of Malonyl-CoA.
• PKS Pathway Integration: Introduce and codon-optimize the genes encoding the key Polyketide Synthase and Cyclase enzymes into the host genome E. coli or yeast.
• Gene Expression and Enzyme Balancing: Tune the expression levels of the PKS and Cyclase to ensure the efficient channeling of the Malonyl-CoA precursor to the cyclization step and minimize intermediate accumulation.
• Fermentation Process Optimization: Develop high-cell-density fed-batch fermentation protocols using cost-effective carbon sources to achieve maximum Phloroglucinol titer and productivity.
• Toxicity and Product Recovery: Engineer the host for improved tolerance to Phloroglucinol and optimize the extraction method for high-purity product isolation.

Technical communication is maintained throughout the process, focusing on timely feedback regarding yield and product quality attributes.

Explore the potential for a sustainable, high-titer Phloroglucinol supply. CD Biosynsis provides customized strain and process engineering solutions:

• Detailed Phloroglucinol Titer and Productivity Reports g/L, g/L/h from optimized fermentation runs.
• Consultation on precursor supply pathway regulation and flux analysis.
• Experimental reports include complete raw data on PKS and Cyclase enzyme activity, precursor pool size, and final product purity.