Glyceraldehyde Engineering Service

Glyceraldehyde is a versatile three-carbon sugar used extensively as a Chemical Intermediate in the synthesis of complex carbohydrates, pharmaceuticals, and chiral molecules. Its production faces significant challenges: High cost and instability of chemical synthesis; limited availability of food-grade product. This limits its use, particularly in high-purity or food-grade applications.

CD Biosynsis focuses on a highly pure, enzymatic route: Enzymatic Cleavage: Use Aldolase enzyme to cleave Fructose-1,6-bisphosphate (or other sugars) into Glyceraldehyde and Dihydroxyacetone Phosphate. This step offers high specificity and yield. Crucially, we utilize a Cell-free system for high purity. This eliminates cellular byproducts, ensuring the production of high-purity, food-grade Glyceraldehyde that is difficult to achieve with traditional chemical or whole-cell fermentation methods.

Pain Points

Glyceraldehyde production is hindered by several chemical and stability issues:

• Instability and Chiral Purity: Glyceraldehyde is highly unstable tending to dimerize and racemize during chemical synthesis, making high-purity chiral product synthesis difficult.
• High Chemical Cost: The traditional chemical oxidation of glycerol requires expensive reagents e.g. catalysts and harsh reaction conditions, driving up production costs.
• Limited Food-Grade Availability: Chemical methods often introduce toxic byproducts, making the final product unsuitable for food or pharmaceutical applications without extensive purification.
• Low Yield and Selectivity: Side reactions and over-oxidation reduce the final yield and selectivity of Glyceraldehyde in chemical processes.

A Cell-free enzymatic system provides the necessary specificity and purity.

Solutions

CD Biosynsis utilizes an enzyme-based, cell-free biomanufacturing platform for Glyceraldehyde:

Aldolase Enzymatic Cleavage

           

We employ high-activity aldolase enzymes to cleave sugar intermediates e.g. Fructose-1,6-bisphosphate into Glyceraldehyde and DHAP, ensuring high selectivity.

Cell-free System for High Purity

We utilize purified enzymes in a cell-free environment to avoid cellular side reactions and byproducts, delivering high purity Glyceraldehyde.

In Situ Product Stabilization

We design the reaction conditions to stabilize Glyceraldehyde against dimerization and racemization, maintaining chiral integrity e.g. D-Glyceraldehyde.

Cofactor Regeneration and Cost Efficiency

If needed, we integrate highly efficient cofactor regeneration systems e.g. NADPH into the cell-free system to reduce operational costs.

This enzymatic approach guarantees high purity, chiral integrity, and sustainability for Glyceraldehyde production.

Advantages

Our Glyceraldehyde engineering service offers these core benefits:

High Chiral Purity

Enzymatic cleavage is highly specific, enabling direct synthesis of chiral Glyceraldehyde e.g. D-Glyceraldehyde without costly chiral separation.

Food-Grade and Pharma-Grade Ready

The cell-free system produces a cleaner product, meeting strict regulatory standards for food and pharmaceutical use.

Avoids Toxic Byproducts

The mild enzymatic reaction replaces harsh chemical oxidizing agents, eliminating toxic byproducts and waste.

Cost-Efficient Enzyme Use

By using a cell-free system, enzymes can be immobilized or recycled efficiently, leading to lower operational costs.

Mild Reaction Conditions

The process operates at near-neutral pH and ambient temperatures, reducing energy consumption and preventing product degradation.

We deliver a sustainable, high-purity solution for Glyceraldehyde supply.

Process

Our Glyceraldehyde engineering service follows a rigorous enzymatic workflow:

• Enzyme Production: Metabolic engineering of E. coli or yeast to overproduce and purify aldolase enzyme for cell-free use.
• Cell-free Reaction Optimization: Establish optimal pH, temperature, and cofactor concentrations for maximal aldolase activity and stability.
• Product Extraction and Stabilization: Develop rapid extraction and in situ stabilization protocols to prevent Glyceraldehyde racemization and degradation.
• Purity Assay: Utilize HPLC and chiral chromatography to validate final Glyceraldehyde purity and enantiomeric excess ee.
• Food-Grade Compliance: Validate absence of solvents, heavy metals, and microbial contaminants to ensure food and pharma safety.

Technical communication is maintained throughout the process, focusing on timely feedback regarding yield and product stability attributes.

Explore the potential for a stable, high-purity Glyceraldehyde supply. CD Biosynsis provides customized strain and process engineering solutions:

• Detailed Glyceraldehyde Titer, Yield, and Enantiomeric Excess (ee) Reports g/L, percent theoretical, percent ee.
• Consultation on downstream chemical conjugation and chiral synthesis applications using the high-purity product.
• Experimental reports include complete raw data on enzyme kinetics, cofactor consumption, and product stability over time.